Gut endoderm is involved in the transfer of left-right asymmetry from the node to the lateral plate mesoderm in the mouse embryo

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Title Gut endoderm is involved in the transfer of left-right asymmetry from the node to the lateral plate mesoderm in the mouse embryo
Publication Type dissertation
School or College School of Medicine
Department Neurobiology & Anatomy
Author Saund, Ranajeet Singh
Date 2013-05
Description Left-right (LR) determination during embryogenesis is critical for the correct positioning of the various visceral organs, such as the lungs and the heart. In establishing LR asymmetry in the mouse, the initial LR signal that is determined in the ciliated embryonic node is transferred to the lateral plate mesoderm (LPM). The cellular and molecular mechanisms for this signal transfer have not been well characterized. Here, we studied the role of endoderm cells in this process by analyzing mouse Sox17 null mutant embryos as a model that develops endoderm-specific defects. Sox17 mutant embryos showed no expression or significantly reduced expression of LR asymmetric genes in the left LPM. A series of experiments revealed the importance of intercellular communication through gap junctions in endoderm cells for LR signal transfer from the node. We also found that SOX17 function is essential not only to form complete epithelial structures that express CX43 and localize connexin proteins on the cell membrane but also to turn off Nodal gene expression in differentiating endoderm cells through a Nodal endoderm-specific enhancer.
Type Text
Publisher University of Utah
Subject MESH Mice; Embryonic Development; Congenital Abnormalities; Gastrulation; Mesoderm; Endoderm; Nodal Signaling Ligands; SOXF Transcription Factors; Cell Differentiation; Right-Left Asymmetry
Dissertation Institution University of Utah
Dissertation Name Doctor of Philosophy
Language eng
Relation is Version of Digital reproduction of Gut Endoderm is Involved in the Transfer of Left-Right Asymmetry from the Node to the Lateral Plate Mesoderm in the Mouse Embryo. Spencer S. Eccles Health Sciences Library. Print version available at J. Willard Marriot Library Special Collections.
Rights Management Copyright © Ranajeet Singh Saund 2013
Format application/pdf
Format Medium application/pdf
Format Extent 4,433,097 bytes
Source Original in Marriott Library Special Collections, QL3.5 2013.S28
ARK ark:/87278/s6s219pd
Setname ir_etd
ID 196400
Reference URL https://collections.lib.utah.edu/ark:/87278/s6s219pd
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