Regulation of eye development by frizzled signaling in Xenopus

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Title Regulation of eye development by frizzled signaling in Xenopus
Publication Type thesis
School or College School of Medicine
Department Neurobiology & Anatomy
Author Rasmussen, Jennifer Terry
Date 2001-08
Description Eye development in both invertebrates and vertebrates is regulated by a network of highly conserved transcription factors. However, it is not known what controls the expression of these factors to regulate early eye formation, and whether transmembrance signaling events are involved. Her we establish a role for signaling via a member of the frizzled family of receptors in regulating early eye development. We show that overexpression of Xenopus frizzled 3 (Xfz3), a receptor expressed during normal eye development, functions cell autonomously to promote ectopic eye formation, and can also perturb endogenous eye development. Ectopic eye obtained with Xfz3 overexperession have a laminar organization similar to endogenous eyes and contain differentiated retinal cell types. Ectopic eye formation is preceded by ectopic expression of transcription factors involved in early eye development, including Pax6, Rx and Otx2. Conversely, targeted overexpression of a dominant-negative form of Xfz3 (Nxfz3), consisting of the soluble extracellular domain of the receptor, results in suppression of endogenous Pax6, Otx2 and Rx expression and suppression of endogenous eye development. This effect can be rescued by co-expression of Xfz3. Finally, overexpression of Kermit, a protein that interacts with the C-terminal intracellular domain of Xfz3, also blocks endogenous eye development, suggesting that signaling through Xfz3 or a related receptor is required for normal eye development. In summary we show that frizzled signaling is both necessary and sufficient to regulate eye development in Xenopus.
Type Text
Publisher University of Utah
Subject Xenopus - Development; Eye - Growth - Regulation
Subject MESH Xenopus; Eye; Growth and Development
Dissertation Institution University of Utah
Dissertation Name MS
Language eng
Relation is Version of Digital reproduction of "Regulation of eye development by frizzled signaling in Xenopus". Spencer S. Eccles Health Sciences Library.
Rights Management © Jennifer Terry Rasmussen.
Format application/pdf
Format Medium application/pdf
Format Extent 1,195,827 bytes
Identifier undthes,3795
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available)
Master File Extent 1,195,864 bytes
ARK ark:/87278/s66w9cvh
Setname ir_etd
ID 190969
Reference URL https://collections.lib.utah.edu/ark:/87278/s66w9cvh
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