| Description |
Plasma membrane tension is a key homeostatic parameter for eukaryotic cells. Therefore, cells need ways to acutely adapt to changes within membrane tensions. Our study suggests the yeast lipid domains called eisosomes can flatten to sense tension during periods of membrane expansion. Additionally, eisosomes house proteins, Nce102 and Slm1, that are sequestered in the domain until flattening occurs, upon which these proteins de-localize. Calcium influx during the acute phase of this response activates downstream calcium signaling pathways that are necessary for Slm1 localization to distinct plasma membrane (PM) sites after exit from the eisosome. Our data also suggest calcium may play an independent, unknown role in regulating PM expansion under alkaline stress. In summary, calcium signaling and eisosomes are interconnected and are central to coordinating both the short- and long-term response to changes in plasma membrane tension. |
