The effect of furosemide on sodium-22 uptake into cerebrospinal fluid and brain

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Title The effect of furosemide on sodium-22 uptake into cerebrospinal fluid and brain
Publication Type thesis
School or College College of Pharmacy
Department Pharmacology & Toxicology
Author Buhrley, Louis Ernest Jr.
Contributor Peart, Helen
Date 1971-06
Description Movement of sodium from plasma into the cerebrospinal fluid (CSF) in against an electrochemical gradient and this process is probably dependent upon active sodium transport. CSF production is inhibited by chemical agents known to interfere with active transport of sodium in the kidney and other systems. Since there are many similarities between renal tubule and choroid plexus epithelial cells, in was of interest to ascertain the effect of a potent saluretic agent on the uptake of radioactive sodium in the CSF and brain. The effect of furosemide on the uptake of sodium-22 into the CSF and cerebral cortex of nephrectomized rats was studies. Furosemide is a potent saluretic agent which abolishes the renal medullary sodium gradient and decreases the short-circuit current in the frog skin and toad bladder. Furosemide, 1 mg/kg, was injected into the left lateral ventricle of the brain, sodium-22 was injected intraperitoneally and the uptake of the isotope into CSF, cerebral cortex, skeletal muscle, and brain was measured at intervals of 0.25 hour after the sodium-22 was administered. During the initial 4 hour period after injection of the sodium-22, the uptake of the isotope was decreased in the furosemide-treated animals. The maximum reduction in uptake occurred at the .0.25-hour time period. The CSSF radioactivity in the treated animals, measured as sodium relative specific activity (RSA), was 37% less than that of the control animals. At 24 hours, there was no difference to sodium-22 uptake in the CSF between the control and treated animals. The uptake of sodium-22 into cerebral cortex was decreased in the treated animals. The largest reduction in activity, 18%, occurred 2 hours after the injection of the isotope. Since the brain extracellular space and the CSF appear to be in relatively free communication, this reduction could be the indirect result of the decreased uptake of sodium-22 into the CSF. Furosemide has slight carbonic anhydrase inhibiting activity but it is probably inadequate to explain fully the reduced uptake of radioactive sodium into the CSF. It is suggested that the decrease in sodium-22 uptake into CSF and brain in the furosemide-treated animals may be caused by inhibition of active sodium transport in the choroid plexus.
Type Text
Publisher University of Utah
Subject Physiological Effect; Diseases
Subject MESH Cerebrospinal Fluid; Furosemide; Sodium
Dissertation Institution University of Utah
Dissertation Name MS
Language eng
Relation is Version of Digital reproduction of "The diurnal variation of norepinephrine in the rat parotid gland." Spencer S. Eccles Health Sciences Library. Print version of "The diurnal variation of norepinephrine in the rat parotid gland." available at J. Willard Marriott Library Special Collection. QP6.5 1972 .S6.
Rights Management © Louis Ernest Buhrley, Jr.
Format application/pdf
Format Medium application/pdf
Format Extent 315,514 bytes
Identifier undthes,5495
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Funding/Fellowship U.S.P.H.S. Pharmacology Training Grant number GM 00153.
Master File Extent 315,554 bytes
ARK ark:/87278/s61v5gr0
Setname ir_etd
ID 190862
Reference URL https://collections.lib.utah.edu/ark:/87278/s61v5gr0
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