Isolation of the secretory component of immunoglobulin A and production of a specific antiserum

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Title Isolation of the secretory component of immunoglobulin A and production of a specific antiserum
Publication Type thesis
School or College College of Nursing
Department Nursing
Author Martin, Sandra Elizabeth Webster
Date 1975-03
Description This study was the initial step in a long range investigation of the local immune system of the urinary tract. The presence of secretory immunoglobulin A in external secretions exercises broad spectrum antimicrobial activity; the high incidence of chronic urinary tract infections, particularly among females, may result from a deficit of this system. As degraded serum immunoglobulin A is excreted in the urine, the fragmented alpha chains would react with the available antiserum to whole secretory immunoglobulin A molecule, and alter the levels of secretory immunoglobulin A assayed. It was therefore necessary to develop a method of determining levels of both secretory immunoglobulin A and secretory component. The secretory component was isolated and partially purified from pooled human colostrum. The major and overwhelming contaminant was lactoferrin. Antisera to these proteins were then developed in rabbits. An attempt was made to separate the rabbit antibodies to the secretory component and the antibodies to the other proteins which were mainly lactoferrin. A double diffusion in gel using the rabbit antisera and tlie antigen to develop antigen-antibody complexes which were then reinjected into rabbits was used. Despite this purification attemp, the final antisera contained a greater quantity of antibodies to the lactoferrin than ti secretory component.
Type Text
Publisher University of Utah
Subject Analysis; Colostrum; Antibodies
Subject MESH Immunoglobulin A, Secretory; Immunization, Passive
Dissertation Institution University of Utah
Dissertation Name MS
Language eng
Relation is Version of Digital reproduction of "Isolation of the secretory component of immunoglobulin A and production of a specific antiserum." Spencer S. Eccles Health Sciences Library. Print version of "Isolation of the secretory component of immunoglobulin A and production of a specific antiserum." available at J. Willard Marriott Library Special Collection. QR6.5 1975 .M3.
Rights Management © Sandra Elizabeth Webster Martin.
Format application/pdf
Format Medium application/pdf
Format Extent 1,162,539 bytes
Identifier undthes,4956
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Master File Extent 1,162,590 bytes
ARK ark:/87278/s6ws8w09
Setname ir_etd
ID 190537
Reference URL https://collections.lib.utah.edu/ark:/87278/s6ws8w09
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