Lymphopoiesis in the adult: identification of early progenitors in both B cell and thymocyte development

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Title Lymphopoiesis in the adult: identification of early progenitors in both B cell and thymocyte development
Publication Type dissertation
School or College School of Medicine
Department Pathology
Author Perry, S. Scott
Contributor Spangrude, Jerry; Pierce, L. Jeanne; Searles, A. Elena; Marx, Melissa; White, Chris; Slayton, William B.; Mojica, Meriluz P.
Date 2002-08
Description The progression of commitment from hematopoietic stem cell to progenitors with specific lineage potentials remains largely undefined, in spite of decades of investigation. This dissertation reports the discovery of a novel hematopoietic progenitor population from adult mouse bone marrow defined as Lin\Neg Sca1\Pos Thy1.1\Neg] This population generates both lymphoid and myeloid cell types, but produces lymphoid forms more frequently in vitro and in transplant assays. It also lacks the ability to rescue lethally irradiated mice that is characteristic of stem cells, but as such, it represents a broad intermediary population in lineage commitment. Immunofluorescent staining and multiparameter flow cytometry supported by functional assays for lineage commitment were employed to separate and enrich the progenitors within this compartment responsible for the differing lineage potentials. cKit, the AA4.1 antigen, and L-selectin were found to distinguish functional subsets within this population, and several progenitors were successfully identified. These included a cKit[\Low AA4.1\ progenitor committed to the B cell lineage, but at a CD45R<super>Neg
Type Text
Publisher University of Utah
Subject Etiology; Myeloid; Lymphoid
Subject MESH Hematopoietic Cell Growth Factors; Stem Cells
Dissertation Institution University of Utah
Dissertation Name PhD
Language eng
Relation is Version of Digital reproduction of "Lymphopoiesis in the adult: identification of early progenitors in both B cell and thymocyte development." Spencer S. Eccles Health Sciences Library. Print version of "Lymphopoiesis in the adult: identification of early progenitors in both B cell and thymocyte development." available at J. Willard Marriott Library Special Collection. RC39.5 2002 .P47.
Rights Management © S. Scott Perry.
Format application/pdf
Format Medium application/pdf
Format Extent 3,137,706 bytes
Identifier undthes,5453
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Funding/Fellowship The George Weber Immunology Research Foundation, the Hematology Training Grant and Lymphoma Foundation provided a graduate student stipened.
Master File Extent 3,137,752 bytes
ARK ark:/87278/s6m32xkp
Setname ir_etd
ID 191562
Reference URL https://collections.lib.utah.edu/ark:/87278/s6m32xkp
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