Copper, zinc-superoxide dismutase: subcellular location in rat liver lysosomes.

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Title Copper, zinc-superoxide dismutase: subcellular location in rat liver lysosomes.
Publication Type dissertation
School or College School of Medicine
Department Biochemistry
Author Geller, Bruce Lee.
Date 1982-12
Description Rat liver Cu,Zn-superoxide dismutase, previously reported to be localized in the mitochondrial intermembrane space in addition to the cytosol, is now found to be lysosomal. The enzyme has been shown to segregate coincidently with lysosomal enzymes and not mitochondrial enzymes from a subcellular granule fraction by three independent methods: (1) digitonin treatment and (2) hypotonic treatment, which selectively releases lysosomal enzymes into the supernate prior to the release of mitochondrial enzymes, and (3) separation of mitochondria and lysosomes by isopycnic density centrifugation after in vivo loading of lysosmes with Triton WR-1339. The Cu,Zn-superoxide dismutase in lysosomes appears to be derived from cytosolic Cu,Zn-superoxide dismutase, since microinjection of the enzyme into HeLa cells results in a partial redistribution from cytosol to lysosomes. In the rat, the amount of superoxide dismutase in the lysosomes varies with the nutritional state of the animal. Fasted animals have about 8% and fed animals about 2% of the total cellular superoxide dismutase in the lysosomes. This increase in lysosomal Cu,Zn-superoxide dismutase upon fasting is consistent with what is known to occur with other soluble cytosolic proteins during autophagy. Cu,Zn-superoxide dismutase is probably not located in mitochondria, as the enzyme found associated with this organelle on isopycnic density gradients may be attributed to lysosomal contamination. However, the possibility that a small amount (less than 0.1% of total cellular) is located in the mitochondria cannot be excluded.
Type Text
Publisher University of Utah
Subject Biology; Lysomes
Subject MESH Superoxide Dismutase; Mitochondria
Dissertation Institution University of Utah
Dissertation Name PhD
Language eng
Relation is Version of Digital reproduction of "Copper, zinc-superoxide dismutase : subcellular location in rat liver lysosomes." Spencer S. Eccles Health Sciences Library. Print version of "Copper, zinc-superoxide dismutase : subcellular location in rat liver lysosomes." available at J. Willard Marriott Library Special Collection. QP 6.5 1982 G44.
Rights Management © Bruce Lee Geller.
Format application/pdf
Format Medium application/pdf
Identifier us-etd2,18591
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Funding/Fellowship The molecular biology student predoctoral training grant form the U.S. Public Health Service, National Institutes of Health. No. GM 07531.
ARK ark:/87278/s6mk6tc3
Setname ir_etd
ID 192498
Reference URL https://collections.lib.utah.edu/ark:/87278/s6mk6tc3
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