Selective metastasis of a transplantable reticulum cell sarcoma

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Title Selective metastasis of a transplantable reticulum cell sarcoma
Publication Type thesis
School or College School of Medicine
Department Neurobiology & Anatomy
Author Parks, Richard Carter
Date 1971-08
Description A transplantable reticulum cell sarcoma, type B, (RCS) which developed spontaneously in a C3Hf mouse, and consistently metastasizes to the spleen was investigated for the purpose of explaining the mechanics of preferential metastasis. Dose-effect studies of the RCS were performed in C3Hf/Pi and (C3Hf x BALB/c) F1 hybrid mice. It was determined that the median survival time of the mice was inversely proportional to the concentration of circulating tumor cells, and that approximately 170 RCS cells were required to induce tumors which would kill 50% of the animals in 30 days. The tumor appeared to develop initially in the splenic red pulp and spread to other organs as the number of RCS cells in the circulation increased. An estimate of the tumor's growth rate in vivo showed the neoplasm's mean generation time to increase cubically with time. Splenectomized mice had a better rate of survival than the sham-operated controls injected intraperitoneally with the same concentration of RCS cells, suggesting that a suitable environment for the early establishment and protection of the neoplasm was probably provided by the spleen. The fate of the RCS and its dissemination in a syngeneic host was examined with an intracellular radioactive label, 1^5iodo-deoxy-uridine (125lUdR). The label had little effect on the biological behavior of the RCS cell. Approximately 90%> of the label's activity was associated with the DNA fraction of the cell. When the RCS cells died almost all of the125i was excreted, thus providing a means for q_uantitating cell loss. Mice were injected intravenously, intraperitoneally and sub-cutaneously with viable and non-viable 125iUdR labelled RCS cells. Anywhere from 60% to 82% (depending on route of injection) of the RCS cells were destroyed within 2h hours post injection. The cell destruction could not he attributable to a specific-immune response. The organs of mice injected intravenously with labelled RCS cells were assayed for the number of tumor cells arrested. An influx of RCS cells in the spleen was observed, while the other organs examined demonstrated a continuous decrease in tumor cells arrested, indicating that the RCS cells were either being arrested and destroyed by the organs or were not being arrested and subsequently destroyed elsewhere. The results of these experiments allow the following conclusions. The RCS has a high degree of transplantability"" with a preference for splenic tissue, subject to its concentration in the circulation. The spleen provides a protective environment suitable for the growth of the neoplasm, since circulating RCS cells not arrested by the spleen are subject to destruction elsewhere. The phenomenon of the RCS's preferential metastasis may be explained on the basis of the specialized growth characteristics of the tumor cell and the organ it is arrested by.""
Type Text
Publisher University of Utah
Subject Tumors; Mice
Subject MESH Neoplasm Metastasis; Lymphoma, Large-Cell
Dissertation Institution University of Utah
Dissertation Name PhD
Language eng
Relation is Version of Digital reproduction of "Selective metastasis of a transplantable reticulum cell sarcoma". Spencer S. Eccles Health Sciences Library. Print version of "Selective metastasis of a transplantable reticulum cell sarcoma." available at J. Willard Marriott Library Special Collection. RC39.5 1971 .P3.
Rights Management © Richard Carter Parks.
Format application/pdf
Format Medium application/pdf
Format Extent 3,553,804 bytes
Identifier undthes,4186
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Funding/Fellowship Research Grant CA05771 from the National Cancer Institute; U.S.P.H.S. and CM0958
Master File Extent 3,553,846 bytes
ARK ark:/87278/s6fb54sv
Setname ir_etd
ID 191184
Reference URL https://collections.lib.utah.edu/ark:/87278/s6fb54sv
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