Separation of mycobacterial soluble antigens

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Title Separation of mycobacterial soluble antigens
Publication Type thesis
School or College School of Medicine
Department Pathology
Author Moulton, Richard Glen
Date 1970-08
Description Ion exchange chromatography and gel filtration have been reported to yield partial separation of mycobacterial antigens. These procedures were used in combination with isoelectric focusing in an attempt to purify antigens of Mycobacterium tuberculosis strain H37Ra. The fractionating action of isoelectric focusing is dependent upon differences in the isoelectric points of the proteins to be separated. Culture filtrate of _M. tubercu1os i s H37Ra was chromatographed on Sephadex G-200. This resulted in 2 widely separated peaks. The first peak, presumably containing high molecular weight substances, was then fractionated on a DEAE Sephadex anion exchange column. Three peaks were collected and each was subjected to isoelectric focusing. Each peak was further separated into 2 or more fractions. The serological reactivity of each fraction was determined by immunodiffusion and immunoe1ectrophoresis. Sensitized guinea pigs were also skin tested with the fractions. Two of the fractions contained only a single precipitinogen. One fraction contained 2 precipitinogens. A fourth fraction contained 3 precipitinogens, and was also the only fraction to display sensitin activity. Four of the fractions were inactive either as precipitinogens or sensitins. The results suggest that the methods described are useful for the separation of mycobacterial antigens.
Type Text
Publisher University of Utah
Subject Bacterial; Polysaccarides
Subject MESH Antigens; Mycobacterium tuberculosis; Proteins
Dissertation Institution University of Utah
Dissertation Name MS
Language eng
Relation is Version of Digital reproduction of "Separation of mycobacterial soluble antigens." Spencer S. Eccles Health Sciences Library. Print version of "Separation of mycobacterial soluble antigens." available at J. Willard Marriott Library Special Collection. QR6.5 1970 .M6.
Rights Management © Richard Glen Moulton.
Format application/pdf
Format Medium application/pdf
Format Extent 1,196,235 bytes
Identifier undthes,4775
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Master File Extent 1,196,282 bytes
ARK ark:/87278/s6319xr7
Setname ir_etd
ID 191775
Reference URL https://collections.lib.utah.edu/ark:/87278/s6319xr7
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