Manipulation and characterization of thin-film interfacial chemistry: sol-gel deposition and single molecule tracking experiments

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Title Manipulation and characterization of thin-film interfacial chemistry: sol-gel deposition and single molecule tracking experiments
Publication Type dissertation
School or College College of Science
Department Chemistry
Author Barhoum, Moussa
Date 2012-05
Description Single molecule trajectories of 1,1'-dioctadecyl-3,3,3'3'-tetramethylindocarbo -cyanine perchlorate (DiI) fluorophores diffusing on planar supported 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) lipid bilayers imaged through total internal reflection fluorescence (TIRF) microscopy at different temperatures are investigated. The spatial resolution limit for detecting molecular motion is evaluated by characterizing the apparent motion which arises from the limited signal-to-noise ratio (S/N) of imaged and simulated stationary DiI molecules. Statistical criteria for reliably distinguishing molecular motion from stationary molecules using F-test statistics, including the computation of local signal-to-noise ratios are then established and used for reliably detecting subdiffraction motion of DiI molecules on DMPC. The same single molecule tracking concept is used in investigating the temperature dependence of subdiffraction diffusional confinement of single Rhodamine 6G molecules in polymer brushes of poly (N-isopropylacrylamide), pNIPAAm, above and below its lower critical solution temperature (LCST) of 32 °C. Reliably distinguishing subdiffraction molecular motion from stationary events is crucial in validating the application of single molecule tracking experiment in probing nanometer-sized hydrophobic environments of polymer structure. A versatile and rapid sol-gel technique for the fabrication of high quality one-dimensional photonic bandgap materials was developed. Silica/titania multilayer materials are fabricated by a sol-gel chemistry route combined with dip-coating onto planar or curved substrate. A shock-cooling step immediately following the thin film heat-treatment process is introduced. The versatility of this sol-gel method is demonstrated by the fabrication of various Bragg stack-type materials with fine-tuned optical properties. Measured optical properties show good agreement with theoretical simulations confirming the high quality of these sol-gel fabricated optical materials. Finally, magnetic functionalization studies of sol-gel derived Co-ion doped titania thin films using superconducting quantum interference device (SQUID) magnetometry and an attempt to measure their magneto-optical properties using a home-built Faraday rotation setup are discussed. The experimental limitations in reliably measuring magnetization responses of these thin films are introduced and discussed in detail. The summary and outlook chapters summarize the scientific significance of each research project and briefly introduce ongoing research based on the work and the results presented in this dissertation.
Type Text
Publisher University of Utah
Subject Interfacial processes; Lipid bilayers; Magneto-optical studies; Single molecule fluorescence; Sol-gel; Thin-deposition
Dissertation Institution University of Utah
Dissertation Name Doctor of Philosophy
Language eng
Rights Management Copyright © Moussa Barhoum 2012
Format application/pdf
Format Medium application/pdf
Format Extent 4,747,780 bytes
Identifier us-etd3,82349
Source Original housed in Marriott Library Special Collections, QD3.5 2012 .B37
ARK ark:/87278/s6v12kjk
Setname ir_etd
ID 194692
Reference URL https://collections.lib.utah.edu/ark:/87278/s6v12kjk
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