Characterization of OCA-B induction in naive CD4+ T cells

Update Item Information
Title Characterization of OCA-B induction in naive CD4+ T cells
Publication Type thesis
School or College School of Medicine
Department Pathology
Author German, Cody
Date 2015-08
Description Oct1 and its transcriptional cofactor OCA-B are required for memory CD4+ T cell development. This study describes OCA-B induction patterns in naïve CD4+ T cells. Naïve CD4+ T cells have no endogenous expression of OCA-B. After 6 hours of in vitro stimulation, by plate bound CD3ε antibodies and CD28 antibodies in media, T cells begin expression of OCA-B. OCA-B expression increases gradually over time with continued stimulation. Maintenance of OCA-B expression is influenced by Oct1 activity: in the absence of Oct1, OCA-B levels are reduced at long time points following stimulation. Additionally, a novel function of OCA-B positively influencing surface expression of CD44, a surface receptor required for memory T cell survival, has been discovered. A floxed, conditional cre-driven knockout allele of OCA-B has been generated in C57/BL6 mice. This allele will prove to be an invaluable tool for researching further in vivo functions of OCA-B.
Type Text
Publisher University of Utah
Subject MESH Octamer Transcription Factor-1; Trans-Activators; Gene Expression; Signal Transduction; Cell Differentiation; T-Lymphocytes; B-Lymphocytes; Genes, Homeobox; Lymphocyte Activation; CD4-Positive T-Lymphocytes; Histone Demethylases; Alleles; Germinal Center; Embryonic Stem Cells; Oxidative Stress; Mice, Knockout; Receptors, Antigen, T-Cell; Antibodies
Dissertation Institution University of Utah
Dissertation Name Master of Science
Language eng
Relation is Version of Digital version of Characterization of OCA-B Induction in Naive CD4+ T Cells
Rights Management Copyright © Cody German 2015
Format application/pdf
Format Medium application/pdf
Format Extent 2,078,668 bytes
Source Original in Marriott Library Special Collections
ARK ark:/87278/s6pc69pg
Setname ir_etd
ID 197361
Reference URL https://collections.lib.utah.edu/ark:/87278/s6pc69pg
Back to Search Results