| Description |
In eukaryotes, lysosomes are dynamic organelles that change their size and shape depending on environmental and biological conditions. An emerging paradigm in lysosome biology is that size and copy number are regulated by an equilibrium between continuous cycles of fusion and fission. We discovered a novel gene, SPAC30D11.06c, that encodes a lysosomal membrane protein. The gene was appropriately named HFL1 (Has Fused Lysosomes) because in the null mutant, perinuclear lysosomes fuse together into long tubules. In this work, we established biochemical conditions to solubilize and purify HFL1 from crude cell lysate and performed a co-immuniprecipitation of HFL1 to identify possible protein partners that might inform on HFL1 function. The results of this study demonstrate that HFL1 can be isolated and studied in vitro providing important tools for future studies exploring HFL1 function. |
