The role of humoral factors in cellular resistance.

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Title The role of humoral factors in cellular resistance.
Publication Type thesis
School or College School of Medicine
Department Pathology
Author Wu, William Gay.
Date 1962-08
Description In vitro experiments were undertaken to determine the role of compliment, properdin and antibody in the phagocytosis and cytopepsis of Histoplasma capsulatum by peritoneal macrophages from normal and immunized mice. Two methods of approach were employed. The first involved the use of freely suspended infected macrophages while the second made use of macrophages attached to glass before infection with the organism. Phagocytic rates were measured by microscopic examination of stained smears in the former method, while supernatant sampling techniques for P-32 activity was employed in the second method. Both procedures involved sampling the medium during incubation to follow cytopepsis. The hypothesis involved was that the P-32 activity appearing in the medium with time was a function of cytopeptic activity of the macrophages on ingested P-32 labeled organisms. Under the experimental condition it was found that rate of phagocytosis were not altered by the presence of various humoral factors studied nor were any differences noted in the rates of ingestion by normal and "immune" populations of cells. The rates of cytopepsis, however, were definitely enhanced by the presence of heat labile factors (i.e., complement and the properdin system, which consists of complement, properdin and Mg++) and less so by antibody, although complement and specific antibody produced increased cytopeptic rates. Studies using complement reagents lacking one or more components failed to reveal which fractions of the heat labile factor might be responsible for enhanced cytopepsis. The "immune" population of cells was found to have increased cytopeptic activity over normal cells in a freely suspended phagocyte system, but not by a fixed macrophage system. This finding points to the importance of the technology involved in biological investigative models.
Type Text
Publisher University of Utah
Subject Antibodies; Cellular Resistance
Subject MESH Immunity; Phagocytes
Dissertation Institution University of Utah
Dissertation Name PhD
Language eng
Relation is Version of Digital reproduction of "The role of humoral factors in cellular resistance." Spencer S. Eccles Health Sciences Library. Print version of "The role of humoral factors in cellular resistance." available at J. Willard Marriott Library Special Collection. QR6.5 1962 .W8.
Rights Management © William Gay Wu.
Format application/pdf
Format Medium application/pdf
Identifier us-etd2,228
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Funding/Fellowship Division of Research Grants and General Medical Scxiences of the National Institutes of Health, United States Public Health Service.
ARK ark:/87278/s6z613q9
Setname ir_etd
ID 193817
Reference URL https://collections.lib.utah.edu/ark:/87278/s6z613q9
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