Synthesis of fluorinated analogs of oxidative DNA lesions and their use to probe features of recognition and repair by base excision repair glycosylases

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Title Synthesis of fluorinated analogs of oxidative DNA lesions and their use to probe features of recognition and repair by base excision repair glycosylases
Publication Type dissertation
School or College College of Science
Department Chemistry
Author Cao, Sheng
Date 2010
Description Damage to DNA occurs through various sources, both exogenous and endogenous. Base lesions resulting from the oxidation of guanine include 8-oxo-7,8-dihydroguanine (OG), guanidinohydantoin (Gh), and spiroiminodihydantoin (Sp). These modified bases can form mismatches and lead to a G - C and G - T transversion mutations in DNA. Fortunately, these lesions are recognized and removed by DNA repair glycosylases found in all organisms. This work explores the features of recognition and repair of these oxidized guanine lesions by a diverse set of base excision glycosylases (MutY, Fpg, hOGG1, Nei, hNEIL1) using synthesized 2' fluorinated nucleosides such as 2'-fluoro-adenosine (FA), 2'-fluoro-8-oxo-guanosine (FOG), 2'-fluoro-guanidinohydan-toin (FGh), and 2'-fluoro-spiroiminodihydantoin (FSp).
Type Text
Publisher University of Utah
Subject DNA lesions; Base excision repair; Glycosylases
Subject LCSH DNA repair; DNA ligases; Molecular recognition
Dissertation Institution University of Utah
Dissertation Name PhD
Language eng
Rights Management ©Sheng Cao
Format application/pdf
Format Extent 4,827,331 bytes
Source Original in Marriott Library Special Collection, QP6.5 2010 .C26
ARK ark:/87278/s6qv422w
Setname ir_etd
ID 193185
Reference URL https://collections.lib.utah.edu/ark:/87278/s6qv422w
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