The biological role of calcium in adult and neonatal neutrophil chemotaxis.

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Title The biological role of calcium in adult and neonatal neutrophil chemotaxis.
Publication Type thesis
School or College School of Medicine
Department Biomedical Informatics
Author Coello, Mary Nell Miller.
Date 1984-06
Description Comparative chemotaxis studies of adult and newborn polymerphonuclear leukocytes (PMNs) were performed by a technique which adapted the micro-pore filter method to a multiwall chamber. Cord blood neutrophils responded to the chemoattractant FMLP with 60% of adult PMN activity. In order to investigate the defect of neonatal PMN chemotaxis as a calcium-associated phenomenon, PMNs wre suspended in a calcium-deprived buffer and in the presence of the calcium channel blocker, verapamil, before being assayed for migration. Adult neutrophils responded to both calcium manipulations with reduced chemotaxis as expected-19% inhibition in calcium-deprived media (p<0.05), and 32% reduction (p<0.001) with 10[-4] M verapamil, Neonatal PMNs, however, increased chemotactic activity in the absence of extracellular calcium-26% (p<0.05) and to a greater extent in the presence of 10[-4] M verapamil (p<0.001). This suggests that the flux of calcium which occurs upon stimulation of the neutrophil by chemotactic factors may, in fact, be responsible for the neonatal defect and the inhibitory concentrations of intracellular calcium may be present in the neonatal cell.
Type Text
Publisher University of Utah
Subject Immunology; Leukocyte
Subject MESH Calcium; Immunity, Cellular; Chemotaxis
Dissertation Institution University of Utah
Dissertation Name MS
Language eng
Relation is Version of Digital reproduction of "The biological role of calcium in adult and neonatal neutrophil chemotaxis." Spencer S. Eccles Health Sciences Library. Print version of "The biological role of calcium in adult and neonatal neutrophil chemotaxis." available at J. Willard Marriott Library Special Collection. RJ 25.5 1984 C63.
Rights Management © Mary Nell Miller Coello.
Format application/pdf
Format Medium application/pdf
Identifier us-etd2,222
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
ARK ark:/87278/s62j6sh5
Setname ir_etd
ID 194087
Reference URL https://collections.lib.utah.edu/ark:/87278/s62j6sh5
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