Expression of yeast DNA mismatch repair proteins and DNA binding characteristics of the yeast condensin Smc2/Smc4 heterodimer

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Title Expression of yeast DNA mismatch repair proteins and DNA binding characteristics of the yeast condensin Smc2/Smc4 heterodimer
Publication Type thesis
School or College School of Medicine
Department Biochemistry
Author Jariangprasert, Suchaiya
Date 2000-08
Description This thesis describes initial experiments on two separate projects. The first project involved the expression and purification of the DNA mismatch repair proteins Mlhl and Pmsl. Expression vectors were made to singly and co-express these two proteins in yeast. Unfortunately, the proteins were found to be insoluble and this project was put on hold. The second project involved studying the DNA binding characteristics of the Smc2/4 core subunits of the yeast condensin complex. During these studies, it was found that the relatively low ionic strength buffer used for most published DNA binding assays caused these proteins to precipitate. In a higher salt buffer that maintained solubility, Smc2/4 heterodimer was found to bind preferentially to M13mpl8 single strand DNA. These results are discussed in light of on-going experiments with the condensin complex.
Type Text
Publisher University of Utah
Subject Genetics; DNA Binding Proteins
Subject MESH DNA Repair; Yeasts; DNA; Protein Binding
Dissertation Institution University of Utah
Dissertation Name MS
Language eng
Relation is Version of Digital reproduction of "©Expression of yeast DNA mismatch repair proteins and DNA binding characteristics of the yeast condensin Smc2/Smc4 heterodimer". Spencer S. Eccles Health Sciences Library. Print version of "Expression of yeast DNA mismatch repair proteins and DNA binding characteristics of the yeast condensin Smc2/Smc4 heterodimer". available at J. Willard Marriott Library Special Collection. QH9.7 2000 .J37.
Rights Management © Suchaiya Jariangprasert.
Format application/pdf
Format Medium application/pdf
Format Extent 1,025,756 bytes
Identifier undthes,4591
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Master File Extent 1,025,798 bytes
ARK ark:/87278/s66w9ct2
Setname ir_etd
ID 190581
Reference URL https://collections.lib.utah.edu/ark:/87278/s66w9ct2
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