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Creator | Title | Description | Subject | Date |
| 76 |
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Dwight, Zachary Lawrence | Laboratory driven, lean-to-adaptive prototyping in Parallel for Web software project identification and application development in health science research | Clinical research laboratories, bioinformatics core facilities, and health science organizations often rely on heavy planning based software development models to propose, build, and distribute software as a consumable product. Projects in non-agile software life cycles tend to have rigid ?plan-desi... | | 2012 |
| 77 |
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Dwight, Zachary Lawrence | uAnalyze: web-based high-resolution DNA melting analysis with comparison to thermodynamic predictions | Abstract-uAnalyzeSM is a web-based tool for analyzing high-resolution melting data of PCR products. PCR product sequence is input by the user and recursive nearest neighbor thermodynamic calculations used to predict a melting curve similar to uMELT (http://www.dna.utah.edu/umelt/umelt.html). Unproce... | | 2012-01-01 |
| 78 |
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Litwin, Christine M. | Anti-tuberculosis IgG antibodies as a marker of active mycobacterium tuberculosis disease | Anti-Mycobacterium tuberculosis IgG antibodies may aid in the diagnosis of active M. tuberculosis disease. We studied whether anti-M. tuberculosis IgG antibodies are elevated in active M. tuberculosis disease and assessed factors contributing to false positive and negative results. A retrospective s... | | 2012-01-01 |
| 79 |
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Wittwer, Carl T. | Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instruments | BACKGROUND: Radioactive DNA polymerase activity methods are cumbersome and do not provide initial extension rates. A simple extension rate assay would enable study of basic assumptions about PCR and define the limits of rapid PCR. METHODS: A continuous assay that monitors DNA polymerase extension us... | | 2014-01-01 |
| 80 |
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Dwight, Zachary Lawrence | Heterozygote PCR product melting curve prediction | Melting curve prediction of PCR products is limited to perfectly complementary strands. Multiple domains are calculated by recursive nearest-neighbor thermodynamics. However, the melting curve of an amplicon containing a heterozygous single nucleotide variant (SNV) after PCR is the composite of four... | | 2014-01-01 |
| 81 |
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Planelles, Vicente | Kinase control of latent HIV-1 infection: PIM-1 kinase as a major contributor to HIV-1 reactivation | Despite the clinical relevance of latent HIV-1 infection as a block to HIV-1 eradication, the molecular biology of HIV-1 latency remains incompletely understood. We recently demonstrated the presence of a gatekeeper kinase function that controls latent HIV-1 infection. Using kinase array analysis w... | | 2014-01-01 |
| 82 |
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Wittwer, Carl T. | Genotyping accuracy of high-resolution DNA melting instruments | High-resolution DNA melting is a closed-tube method for genotyping and variant scanning that depends on the thermal stability of PCR-generated products. Instruments vary in thermal precision, sample format, melting rates, acquisition, and software. Instrument genotyping accuracy has not been assesse... | | 2014-01-01 |
| 83 |
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Rockwood, Alan L. | Performance enhancement in the measurement of 5 endogenous steroids by LC-MS/MS combined with differential ion mobility spectrometry | Background: Challenges for steroid analysis by LC-MS/MS include low ionization efficiency, endogenous isobars with similar fragmentation patterns and chromatographic retention. Differential ion mobility spectrometry (DMS) provides an additional degree of separation prior to MS/MS detection, and show... | | 2014-01-01 |
| 84 |
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Brooking, Haisley; Bernard, Philip; Skliar, Mikhail; Zhang, Huanan | Characterization of exosomes | Exosomes are nano-vesicles secreted by cells. They are found in extracellular space and all body fluids. This poster describes the methods for the isolation and characterization of the exosomes and my plans for future research. | Cancer--Research | 2017 |
