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Creator | Title | Description | Subject | Date |
| 51 |
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Normann, Richard A.; Chandler, John P. | Effects of calcium ions on L-type horizontal cells in the isolated turtle retina | A technique by which the retina can be isolated from the turtle eye is described. Scanning electron microscopy revealed morphological variability between preparations and also between regions of the same one. Large areas were often totally free of any pigment epithelial cells, yet contained a high p... | Retina; Calcium; Horizontal Cell; Turtle | 1990 |
| 52 |
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Jaskowski, Troy D. | Evaluation of a PCR probe capture assay for the detection of Toxoplasma gondii: incorporation of uracil N-glycosylase for contamination control | Toxoplasma gondii is a cyst-forming parasite of clinical relevance in humans primarily because of the neurologic abnormalities it can cause. In some clinical circumstances, it is desirable to detect the pathogen directly. We modified a commercially available Toxoplasma polymerase chain reaction... | PCR probe capture assay; Uracil N-glycosylase; UNG | 2000 |
| 53 |
 |
Pounder, June I.; Voelkerding, Karl V.; Storts, D.; Anderson, Clint M. | Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysis | Differentiation of members within Mycobacterium tuberculosis complex (MTC) is essential for patient management and infection control. MTC is comprised of M. tuberculosis (Mtb), M. bovis (Mb), M. bovis BCG (vaccine strain; Mbcg), M. africanum (Ma), and M. microti (Mm). Because MTC have highly similar... | MTC; species differentiation; Mycobacterium bovis BCG; Mycobacterium africanum; Mycobacterium microtic; PCR | 2006-01-09 |
| 54 |
 |
Rockwood, Alan L. | Mass spectrometry for steroids, immunosuppressants, and other clinical applications | Presentation slides on matching mass spectrometry technology to clinical applications. | Immunosuppressants | 2005-07-26 |
| 55 |
 |
Aldous, Jay A.; Engar, Richard C. | Do dentists prescribe narcotics excessively? | Dealing with pain is an inevitable sequela to dental treatment. Although several drug regimens primarily involving narcotics have been used in the past, availability of nonsteroidal anti-inflammatory drugs (NSAIDs) has increased recently. A study was conducted to analyze dental prescribing patterns ... | Analgesics, Opioid; Anti-Inflammatory Agents; Dentist's Practice Patterns; Pain, Postoperative | 1996-07-01 |
| 56 |
 |
Mongia, Shella K.; Rawlins, Mindy, L.; Owen, William E.; Roberts, William L. | Performance characteristics of seven automated CA125 assays | Author manuscript. Cancer antigen 125 (CA125) is a high molecular mass glycoprotien, which is used as tumor marker to monitor disease progression, response to therapy and in early detection of recurrence after treatment for ovarian cancer. The Access 2, ADVIA Centaur, ARCHITECT i2000, AxSYM, Elecsys... | CA125 assays | 2005-08-29 |
| 57 |
 |
Jaskowski, Troy D. | Comparison of four enzyme immunoassays with a western blot assay for the determination of type-specific antibodies to herpes simplex virus | Most current enzyme immunoassays (EIAs) differentiate inadequately between types 1 and 2 herpes simplex virus (HSV) antibodies since significant crossreactivity exists. We compared 4 IgG type-specific EIAs using a Western blot assay for resolution of discrepant results. The Diamedix had sensitivit... | Enzyme immunoassays; Western blot assay; Type-specific antibodies | 2001 |
| 58 |
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Coffin, Cheryl M.; Capecchi, Mario R. | Alveolar rhabdomyosarcomas in conditional Pax3:Fkhr mice: cooperativity of Ink4a/ARF and Trp53 loss of function. | Alveolar rhabdomyosarcoma is an aggressive childhood muscle cancer for which outcomes are poor when the disease is advanced. Although well-developed mouse models exist for embryonal and pleomorphic rhabdomyosarcomas, neither a spontaneous nor a transgenic mouse model of alveolar rhabdomyosarcoma has... | Cell Differentiation; Forkhead Transcription Factors; Myogenic Regulatory Factors | 2004-11-01 |
| 59 |
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Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R. | Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) and COBAS HCV TaqMan ASR Assays using the Vidiera NSP sample preparation instrument. | The Vidiera Nsp nucleic sample preparation instrument (Vidiera,Beckman-Coulter Inc., Fullerton CA) was evaluated using the COBAS HBV TaqMan Analyte Specific Reagent (ASR. Roche Molecular Diagnostics [RMD], Indianapolis, IN) and COBAS HCV TaqMan ASR (RMD) assays using clinical samples and HCV Armored... | Quantitative Tests; Linearity Studies | 2006-05-05 |
| 60 |
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Owen, William E.; Roberts, William L. | Effects of hemoglobin C and S traits on the results of 14 commercial glycated hemoglobin assays | Glycated hemoglobin is widely used in the management of diabetes mellitus. At least 300,000 Americans with diabetes mellitus have the hemoglobin (Hb) C or S trait. The accuracy of HbA1c methods can be adversely affected by the presence of these traits. We evaluated the effects of HbC and HbS traits ... | Glycated hemoglobin assays; Hemoglobin C trait; Hemoglobin S trait | 2008 |
| 61 |
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Petti, Cathy A.; Polage, Christopher R. | Assessment of the utility of viral culture of cerebrospinal fluid | Nucleic acid amplification testing is the preferred method to detect enteroviruses and Herpesviridae in cerebrospinal fluid, but clinicians still request viral culture. Review of 22,394 viral cultures of cerebrospinal fluid samples found that <0.1% recovered nonenterovirus, non-Herpesviridae species... | Enterovirus Infections; Herpesviridae Infections; Virus Cultivation | 2006-12-15 |
| 62 |
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Hymas, Weston C.; Hillyard, David R. | Description and validation of a novel real-time RT-PCR enterovirus assay | Enteroviruses are a leading cause of aseptic meningitis in adult and pediatric populations. We describe the development of a real-time RT-PCR assay that amplifies a small target in the 5' nontranslated region upstream of the classical Rotbart enterovirus amplicon. The assay includes an RNA internal ... | RT-PCR enterovirus assay; Rotbart enterovirus amplicon; Aseptic meningitis | 2008 |
| 63 |
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Digre, Kathleen B.; Gouw, Launce G.; Harris, Catherine P.; Haines, John H.; Ptacek, L.J. | Autosomal dominant cerebellar ataxia with retinal degeneration: clinical, neuropathologic, and genetic analysis of a large kindred. | The autosomal dominant cerebellar ataxias (ADCA) comprise a heterogeneous group of neurologic disorders characterized by degeneration of the cerebellum, spinal cord, and brainstem. Genetic analysis has revealed two loci, SCA1 on chromosome 6p, and SCA2 on chromosome 12q, responsible for some ADCA. W... | Retinal Degeneration; Cerebellar Ataxia; Genetic Analysis | 1994-08 |
| 64 |
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Owen, William E.; Martins, Thomas B.; Litwin, Christine M.; Roberts, William L. | Performance characteristics of six IMMULITE 2000 TORCH assays | TORCH is an acronym for Toxoplasma gondii (Toxo), other microorganisms (like syphilis), rubella (RV), cytomegalovirus (CMV), and herpesviruses (HSV) that are associated with congenital abnormalities during maternal infection. Prenatal testing for antibodies against these agents is one tool for preve... | TORCH; Toxoplasma gondii; syphilis virus; rubella; cytomegalovirus; herpesvirus; IMMULITE 2000; congenital abnormalities; laboratory testing | 2006-01-04 |
| 65 |
 |
Owen, William E.; Roberts, William L. | Performance characteristics of eight estradiol immunoassays | Measurement of estradiol is useful in assisted reproduction, evaluation of infertility, menopause, and male feminization. The analytic performance of 8 estradiol immunoassays was evaluated. The imprecision and accuracy of the Access, ADVIA Centaur, ARCHITECT i2000, AutoDELFIA, Elecsys 2010, IMMULI... | Estradiol immunoassays | 2004 |
| 66 |
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Owen, William E.; Roberts, William L. | Performance characteristics of four automated natriuretic peptide assays | Measurement of circulating B-type natriuretic peptide (BNP) and N-terminal proBNP (NT-proBNP) can identify patients with heart failure and guide therapy. The limit of detection, linearity, imprecision, method comparison, analytic concordance, and reference intervals of the Access 2 BNP (Biosite, San... | Natriuretic peptide assays; BNP | 2005 |
| 67 |
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Singleton, J. Robinson; Smith, A. Gordon | Microvascular complications of impaired glucose tolerance. | Impaired glucose tolerance (IGT) serves as a marker for the state of insulin resistance and predicts both large- and small-vessel vascular complications, independent of a patient's progression to diabetes. Patients with IGT are at significantly increased risk for death and morbidity due to myocardia... | Microcirculation; Humans | 2003-12-01 |
| 68 |
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Hymas, Weston C.; Hillyard, David R. | Real time RT-PCR assay for norovirus detection with eclipse probes and a non-competitive internal control. | Noroviruses are the major causative agents of nonbacterial gastroenteritis worldwide and are estimated to cause approximately 23 million cases in the United States annually. Real time RT-PCR is rapidly becoming the principle diagnostic means for norovirus detection due to its enhanced sensitivity an... | Gastroenteritis; Eclipse Hybridization Probes; Norovirus | 2005-05-03 |
| 69 |
 |
Hymas, Weston C.; Hillyard, David R. | Novel one step real time RT-PCR Assay for the detection of Enterovirus | nteroviruses (EV) are the leading cause of aseptic meningitis in pediatric and adult populations and can be associated with severe disease such as myocarditis, encephalitis, and paralytic poliomyelitis. RT-PCR has rapidly become the diagnostic methodology of choice due to its sensitivity and rapid t... | Viruses; Isolation; Purification | 2005-07-11 |
| 70 |
 |
Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R. | Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) Assay and Comparison to the COBAS Amplicor HBV Monitor Assay. | erformance characteristics of the COBAS HBV TaqMan ASR (TaqMan) assay were evaluated and compared to the COBAS Amplicor HBV Monitor test (Amplicor) assay for the quantification of HBV DNA in clinical samples. | Quantitative Tests; Linearity Studies | 2004-05-03 |
| 71 |
 |
Aldous, Wade K.; Hymas, Weston C.; Stevenson, Jeffery B.; Taggart, Edward W.;Hillyard, David R. | Single tube real-time RT-PCR Assay for the detection of enterovirus | We developed a rapid and sensitive method for the routine detection of enterovirus in CSF and plasma using a one tube, one step, real-time PCR assay. A primer set and an Eclipse probe were designed to target the highly conserved 5' untranslated region of the enterovirus genome. This assay was compar... | MGB Eclipse; Viral RNA; Picornaviridae | 2004-09-11 |
| 72 |
 |
Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R. | Evaluation of the Alliance HCV Quantitative Analyte Specific Reagent (ASR) Assay and Comparison to the COBAS HCV TaqMan ASR Assay. | To evaluate the Abbott/Celera Alliance HCV Quantitative ASR (Alliance) assay using the Qiagen BioRobot 9604 and QiaAmp Virus Kit for viral RNA isolation. And compare Alliance HCV TaqMan ASR to the Roche COBAS HCV TaqMan ASR (CTM) assay calibrated using Armored RNA solutions and WHO 2nd international... | Quantitative Tests; Linearity Studies | 2004-05-10 |
| 73 |
 |
Konnick, Eric Q.; Williams, Sheri M.; Ashwood, Edward R.; Hillyard, David R. | Performance Characteristics of the COBAS HCV TaqMan ASR Using Armored RNA Calibrators | As improved therapies have become available for Hepatititis C Virus (HCV) infection, the use of assays to quantitate HCV RNA has increased dramatically (3, 14, 16). The initial use of these assays was to predict the likelihood of therapy based on baseline HCV RNA levels. These reports indicated that... | Roche Amplicor Monitor; NGI Superquant; patients | 2003-04-22 |
| 74 |
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Konnick, Eric Q.; Williams, Sheri M.; Ashwood, Edward R; Hillyard, David R | Performance characteristics of the COBAS HCV TaqMan ASR and Comparison to the COBAS Amplicor HCV Monitor, Version 2.0 and Versant HCV bDNA 3.0. | Evaluate the COBAS HCV TaqMan ASR assay using both the Qiagen BioRobot 9604 and a 1mL extraction protocol for HCV RNA isolation. And Compare the COBAS TaqMan HCV ASR assay to the COBAS Amplicor HCV Monitor, Version 2.0 and Versant HCV bDNA 3.0 assays. | Quantitative Tests; Linearity Studies | 2002-11-21 |
| 75 |
 |
Hymas, Weston C.; Stevenson, Jeffery B.; Taggart, Bill; Hillyard, David R. | Quantitative real time PCR assay for the detection of human herpes 6. | Human herpes virus type 6 is a beta virus that occurs as two variants (A & B) and infects nearly 100% of the population before two years of age. Primary infections in children can produce fever and a unique lacy rash that gives the disease its name: roseola. The rare primary infection in adults can ... | Probe Technology; Roseola Infantum | 2005-02-05 |
