|
|
Creator | Title | Description | Subject | Date |
| 51 |
 |
Cloud, Joann L.; Dunn, Jim; Hoggan, Karen; Wilson, Deborah; Iwen, Peter; Lasala, Paul; Hall, Geri; Woods, Gail L. | Sequence-based identification of gram negative nonfermenters using commercial 16S rDNA databases | This poster presents a study assessing the validity of the databases from two commercial systems were compared for the identification of NF. Background: Identification of gram negative nonfermenters (NF) from cultured human specimens is often difficult using traditional phenotypic methods. Partial 1... | MicroSeq500; Virodec | 2005-08-29 |
| 52 |
 |
Mongia, Shella K.; Rawlins, Mindy, L.; Owen, William E.; Roberts, William L. | Performance characteristics of seven automated CA125 assays | Author manuscript. Cancer antigen 125 (CA125) is a high molecular mass glycoprotien, which is used as tumor marker to monitor disease progression, response to therapy and in early detection of recurrence after treatment for ovarian cancer. The Access 2, ADVIA Centaur, ARCHITECT i2000, AxSYM, Elecsys... | CA125 assays | 2005-08-29 |
| 53 |
 |
Cloud, Joann L.; Hoggan, Karen; Cohen, Samuel; Brown-Elliott, Barbara A.; Mann, Linda; Wilson, Rebecca; Aldous, Wade K.; Wallace, Jr, Richard J.; Woods, Gail L. | Differentiation of mycobacterium chelonae and M. abscessus using SmartCycler PCR and MGB eclipse probes | This poster discusses how the use of partial 16S rDNA sequencing (first one-third of the gene) for the identification of Mycobacterium species from cultured isolates has become recognized as a very accurate method for identification. The distinction between M. chelonae and M. abscessus, however, is ... | MGB Eclipse System; SmartCycler PCR; Mycobacterium abscessus | 2005-09-05 |
| 54 |
 |
Parker, Sam; Stevenson, Jeffery B.; Hillyard, David R. | Eclipse probe assay for the detection of mycoplasma pneumoniae. | Mycoplasma pneumoniae is an important human pathogen that causes community acquired pneumonias and upper respiratory tract infections. Traditional detection techniques, such as culture and serology, cannot rapidly aid in the diagnosis of an acute infection with adequate sensitivity and specificity. ... | Eclipse Hybridization Probe; Protocol; Techniques | 2005-09-11 |
| 55 |
 |
Erickson, J. Alan; Aldeen, William E.; Ashwood, Edward R. | Evaluation of a fecal pancreatic elastase-1 enzyme-linked immunosorbent assay: assessment versus an established assay and implication in classifying pancreatic function | Disagreement continues regarding the two commercially available fecal pancreatic elastase-1 (PE-1) ELISAs and their respective capabilities to assess pancreatic function. Our objectives were to validate the newer PE-1 ELISA and evaluate the test against the previously established assay, to investiga... | PE-1; pancreatic elastase-1; FDA; AMR; analytical measurement range; BioServ Diagnostics PE-1ELISA; ScheBo Biotech PE-1ELISA | 2005-09-25 |
| 56 |
 |
Margraf, Rebecca L.; Mao, Rong; Wittwer, Carl T. | Masking techniques: masking selected sequence variation by incorporating mismatches into melting analysis probes | Hybridization probe melting analysis can be complicated by the presence of sequence variation (non-pathogenic polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Materials and Meth... | probe hybridization; high-resolution melting analysis; masking; RET | 2005-10-21 |
| 57 |
 |
Herrmann, Mark G.; Durtschi, Jacob; Bromley, L. Kathryn; Voelkerding, Karl V. | Cross platform comparison of melting curve analysis | Melting analysis of PCR product was first performed on the LightCycler 10 years ago. Now, melting analysis is a standard function on all real-time PCR instruments. Recent advances in DNA melting analysis, including high resolution melting and specialized dyes, have increased the capabilities of melt... | PCR; SNP; SYBR Green I; LC Green Plus; high-resolution melting; mutation scanning; amplicon genotyping | 2005-11-04 |
| 58 |
 |
Hymas, Weston C.; Hillyard, David R. | Novel one step real time RT-PCR assay for the detection of Enterovirus, A | This poster describes the development of a novel real time assay that utilizes primers and an Eclipse probe in an overlapping format upstream of the Rotbart amplicon. | RT-PCR Assay | 2005-11-08 |
| 59 |
 |
Cloud, Joann L.; Meyer, Jay J.; Pounder, June I.; Woods, Gail L. | Mycobacterium arupense sp. nov., a novel moderately growing nonchromogenic bacterium isolated from clinical specimens | This author manuscript discusses how several isolates of Mycobacterium species related to the M. terrae complex have been isolated from clinical samples. In the clinical microbiology laboratory, partial 16S rRNA gene sequencing (approximate first 500 base pairs) is often used to identify Mycobacteri... | Mycobacterium arupense; Mycobacterium nonchromogenicum; Mycobacterium terrae; Mycobacterium triviale; MCRO 6 | 2005-11-22 |
| 60 |
 |
Erickson, J. Alan; Smith, Jeffrey, J.; Bornhorst, Joshua A.; Ashwood, Edward R. | Humananti-mouse antibody protected ELISA for the quantification of squamous cell carcinoma antigen | Squamous cell carcinoma antigen (SCC) was first isolated from squamous cell carcinoma tissue of the uterine cervix and initially reported as TA-4. The antigen has been characterized as a glycoprotein with a molecular weight between 45,000 and 55,000 daltons. Studies suggest that SCC may function as ... | SCC; Squamous Cell Carcinoma Antigen; BSA; PBS; Phosphate Buffered Saline; HAMA; Human Anti-mouse Antibodies; IIR; ImmunoglobulinInhibiting Reagent | 2005-12-09 |
| 61 |
 |
Owen, William E.; Rockwood, Alan L.; Roberts, William L.; Meikle, A. Wayne | Development and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroids | Congenital adrenal hyperplasia is a group of autosomal recessive disorders caused by a deficiency of 1 of 4 enzymes required for the synthesis of glucocorticoids, mineralocorticoids, and sex hormones. Analysis of 11-deoxycortisol (11DC), 17-hydroxyprogesterone (17OHP), 17-hydroxypregnenolone (17O... | Congenital adrenal hyperplasia; Adrenal steroids; Tandem mass spectrometry assay | 2006 |
| 62 |
 |
Petti, Cathy A. | Role of the laboratory in diagnosis of influenza during seasonal epidemics and potential pandemics | Laboratory diagnosis of influenza is critical to its treatment and surveillance. With the emergence of novel and highly pathogenic avian influenza viruses, the role of the laboratory has been further extended to include isolation and subtyping of the virus to monitor its appearance and facilitate ap... | Antibodies, Viral; Antigens, Viral; RNA, Viral | 2006 |
| 63 |
 |
Owen, William E.; Martins, Thomas B.; Litwin, Christine M.; Roberts, William L. | Performance characteristics of six IMMULITE 2000 TORCH assays | TORCH is an acronym for Toxoplasma gondii (Toxo), other microorganisms (like syphilis), rubella (RV), cytomegalovirus (CMV), and herpesviruses (HSV) that are associated with congenital abnormalities during maternal infection. Prenatal testing for antibodies against these agents is one tool for preve... | TORCH; Toxoplasma gondii; syphilis virus; rubella; cytomegalovirus; herpesvirus; IMMULITE 2000; congenital abnormalities; laboratory testing | 2006-01-04 |
| 64 |
 |
Pounder, June I.; Simmon, Keith E.; Barton, Claudia A.; Hohmann, Sheri L. | Identification of nonsporulating molds by sequencing internal transcribed spacer regions with Virodec software and database | Fungal infections are increasing, particularly among immunocompromised hosts, and a rapid, accurate diagnosis is essential for the initiation of targeted antifungal therapy. Identification of fungi from culture requires the presence of reproductive structures, and the absence of spores can increase ... | nonsporulating molds; genetic sequencing; Virodec; ITS I; ITS II | 2006-01-09 |
| 65 |
 |
Pounder, June I.; Voelkerding, Karl V.; Storts, D.; Anderson, Clint M. | Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysis | Differentiation of members within Mycobacterium tuberculosis complex (MTC) is essential for patient management and infection control. MTC is comprised of M. tuberculosis (Mtb), M. bovis (Mb), M. bovis BCG (vaccine strain; Mbcg), M. africanum (Ma), and M. microti (Mm). Because MTC have highly similar... | MTC; species differentiation; Mycobacterium bovis BCG; Mycobacterium africanum; Mycobacterium microtic; PCR | 2006-01-09 |
| 66 |
 |
Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R. | Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) and COBAS HCV TaqMan ASR Assays using the Vidiera NSP sample preparation instrument. | The Vidiera Nsp nucleic sample preparation instrument (Vidiera,Beckman-Coulter Inc., Fullerton CA) was evaluated using the COBAS HBV TaqMan Analyte Specific Reagent (ASR. Roche Molecular Diagnostics [RMD], Indianapolis, IN) and COBAS HCV TaqMan ASR (RMD) assays using clinical samples and HCV Armored... | Quantitative Tests; Linearity Studies | 2006-05-05 |
| 67 |
 |
Smith, A. Gordon; Singleton, J. Robinson | Lifestyle intervention for pre-diabetic neuropathy. | OBJECTIVE: The purpose of this study was to evaluate intraepidermal nerve fiber density (IENFD) as a sensitive measure of neuropathy change in patients with neuropathy associated with impaired glucose tolerance (IGT) receiving lifestyle intervention based on that used in the Diabetes Prevention Prog... | Older people; Biopsy; Blood Pressure; Body Mass Index; Cholesterol; Diabetic Diet | 2006-06-29 |
| 68 |
 |
Petti, Cathy A.; Polage, Christopher R. | Assessment of the utility of viral culture of cerebrospinal fluid | Nucleic acid amplification testing is the preferred method to detect enteroviruses and Herpesviridae in cerebrospinal fluid, but clinicians still request viral culture. Review of 22,394 viral cultures of cerebrospinal fluid samples found that <0.1% recovered nonenterovirus, non-Herpesviridae species... | Enterovirus Infections; Herpesviridae Infections; Virus Cultivation | 2006-12-15 |
| 69 |
 |
Owen, William E.; Roberts, William L.; Ring, Terry Arthur | Development and validation of an automated thawing and mixing workcell | Working toward a goal of total laboratory automation, we are automating manual activities in our highest volume laboratory section. Because half of all specimens arriving in this laboratory section are frozen, we began by developing an automated workcell for thawing frozen specimens and mixing the t... | Automated workcell; Laboratory automation | 2007 |
| 70 |
 |
Owen, William E.; Roberts, William L. | Effects of hemoglobin (Hb) E and HbD traits on measurements of glycated Hb (HbA1c) by 23 methods. | Glycohemoglobin (GHB), reported as hemoglobin (Hb) A1c, is a marker of long-term glycemic control in patients with diabetes and is directly related to risk for diabetic complications. HbE and HbDare the second and fourth most common Hb variants worldwide. We investigated the accuracy of HbA1c measur... | Glycohemoglobin; GHB; Glycated Hb; Glycemic control | 2008 |
| 71 |
 |
Owen, William E.; Roberts, William L. | Effects of hemoglobin C and S traits on the results of 14 commercial glycated hemoglobin assays | Glycated hemoglobin is widely used in the management of diabetes mellitus. At least 300,000 Americans with diabetes mellitus have the hemoglobin (Hb) C or S trait. The accuracy of HbA1c methods can be adversely affected by the presence of these traits. We evaluated the effects of HbC and HbS traits ... | Glycated hemoglobin assays; Hemoglobin C trait; Hemoglobin S trait | 2008 |
| 72 |
 |
Hymas, Weston C.; Hillyard, David R. | Description and validation of a novel real-time RT-PCR enterovirus assay | Enteroviruses are a leading cause of aseptic meningitis in adult and pediatric populations. We describe the development of a real-time RT-PCR assay that amplifies a small target in the 5' nontranslated region upstream of the classical Rotbart enterovirus amplicon. The assay includes an RNA internal ... | RT-PCR enterovirus assay; Rotbart enterovirus amplicon; Aseptic meningitis | 2008 |
| 73 |
 |
Jaskowski, Troy D. | Diagnostic performance of phospholipid-specific assays for the evaluation of antiphospholipid syndrome | The diagnostic performance of commercially available nonstandard antiphospholipid (aPL) assays for the evaluation of antiphospholipid syndrome (APS) is unknown. In 62 patients with APS, 88 with recurrent pregnancy loss, 50 healthy blood donors, and 24 women with one or more successful pregnancies, w... | APS; Phospholipid-specific assays; Diagnostic performance | 2008-06-01 |
| 74 |
 |
Dwight, Zachary Lawrence | uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application | uMeltSM is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR products. The user defines an amplicon sequence and chooses a set of thermodynamic and experimental parameters that include nearest-neighbor stacking energies, loop entropy effects, cation (mono... | | 2011 |
| 75 |
 |
Roberts, William L. | Specificity characteristics of 7 commercial creatinine measurement procedures by enzymatic and jaffe method principles | Standardized calibration does not change a creatinine measurement procedure?s susceptibility to potentially interfering substances. | | 2012 |
