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51 Hymas, Weston C.; Hillyard, David R.Novel one step real time RT-PCR Assay for the detection of Enterovirusnteroviruses (EV) are the leading cause of aseptic meningitis in pediatric and adult populations and can be associated with severe disease such as myocarditis, encephalitis, and paralytic poliomyelitis. RT-PCR has rapidly become the diagnostic methodology of choice due to its sensitivity and rapid t...Viruses; Isolation; Purification2005-07-11
52 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) Assay and Comparison to the COBAS Amplicor HBV Monitor Assay.erformance characteristics of the COBAS HBV TaqMan ASR (TaqMan) assay were evaluated and compared to the COBAS Amplicor HBV Monitor test (Amplicor) assay for the quantification of HBV DNA in clinical samples.Quantitative Tests; Linearity Studies2004-05-03
53 Aldous, Wade K.; Hymas, Weston C.; Stevenson, Jeffery B.; Taggart, Edward W.;Hillyard, David R.Single tube real-time RT-PCR Assay for the detection of enterovirusWe developed a rapid and sensitive method for the routine detection of enterovirus in CSF and plasma using a one tube, one step, real-time PCR assay. A primer set and an Eclipse probe were designed to target the highly conserved 5' untranslated region of the enterovirus genome. This assay was compar...MGB Eclipse; Viral RNA; Picornaviridae2004-09-11
54 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.Evaluation of the Alliance HCV Quantitative Analyte Specific Reagent (ASR) Assay and Comparison to the COBAS HCV TaqMan ASR Assay.To evaluate the Abbott/Celera Alliance HCV Quantitative ASR (Alliance) assay using the Qiagen BioRobot 9604 and QiaAmp Virus Kit for viral RNA isolation. And compare Alliance HCV TaqMan ASR to the Roche COBAS HCV TaqMan ASR (CTM) assay calibrated using Armored RNA solutions and WHO 2nd international...Quantitative Tests; Linearity Studies2004-05-10
55 Konnick, Eric Q.; Williams, Sheri M.; Ashwood, Edward R.; Hillyard, David R.Performance Characteristics of the COBAS HCV TaqMan ASR Using Armored RNA CalibratorsAs improved therapies have become available for Hepatititis C Virus (HCV) infection, the use of assays to quantitate HCV RNA has increased dramatically (3, 14, 16). The initial use of these assays was to predict the likelihood of therapy based on baseline HCV RNA levels. These reports indicated that...Roche Amplicor Monitor; NGI Superquant; patients2003-04-22
56 Konnick, Eric Q.; Williams, Sheri M.; Ashwood, Edward R; Hillyard, David RPerformance characteristics of the COBAS HCV TaqMan ASR and Comparison to the COBAS Amplicor HCV Monitor, Version 2.0 and Versant HCV bDNA 3.0.Evaluate the COBAS HCV TaqMan ASR assay using both the Qiagen BioRobot 9604 and a 1mL extraction protocol for HCV RNA isolation. And Compare the COBAS TaqMan HCV ASR assay to the COBAS Amplicor HCV Monitor, Version 2.0 and Versant HCV bDNA 3.0 assays.Quantitative Tests; Linearity Studies2002-11-21
57 Hymas, Weston C.; Stevenson, Jeffery B.; Taggart, Bill; Hillyard, David R.Quantitative real time PCR assay for the detection of human herpes 6.Human herpes virus type 6 is a beta virus that occurs as two variants (A & B) and infects nearly 100% of the population before two years of age. Primary infections in children can produce fever and a unique lacy rash that gives the disease its name: roseola. The rare primary infection in adults can ...Probe Technology; Roseola Infantum2005-02-05
58 Parker, Sam; Stevenson, Jeffery B.; Hillyard, David R.Eclipse probe assay for the detection of mycoplasma pneumoniae.Mycoplasma pneumoniae is an important human pathogen that causes community acquired pneumonias and upper respiratory tract infections. Traditional detection techniques, such as culture and serology, cannot rapidly aid in the diagnosis of an acute infection with adequate sensitivity and specificity. ...Eclipse Hybridization Probe; Protocol; Techniques2005-09-11
59 Cloud, Joann L.; Hoggan, Karen; Cohen, Samuel; Brown-Elliott, Barbara A.; Mann, Linda; Wilson, Rebecca; Aldous, Wade K.; Wallace, Jr, Richard J.; Woods, Gail L.Differentiation of mycobacterium chelonae and M. abscessus using SmartCycler PCR and MGB eclipse probesThis poster discusses how the use of partial 16S rDNA sequencing (first one-third of the gene) for the identification of Mycobacterium species from cultured isolates has become recognized as a very accurate method for identification. The distinction between M. chelonae and M. abscessus, however, is ...MGB Eclipse System; SmartCycler PCR; Mycobacterium abscessus2005-09-05
60 Dwight, Zachary LawrenceLaboratory driven, lean-to-adaptive prototyping in Parallel for Web software project identification and application development in health science researchClinical research laboratories, bioinformatics core facilities, and health science organizations often rely on heavy planning based software development models to propose, build, and distribute software as a consumable product. Projects in non-agile software life cycles tend to have rigid ?plan-desi...2012
61 Petti, Cathy A.Role of the laboratory in diagnosis of influenza during seasonal epidemics and potential pandemicsLaboratory diagnosis of influenza is critical to its treatment and surveillance. With the emergence of novel and highly pathogenic avian influenza viruses, the role of the laboratory has been further extended to include isolation and subtyping of the virus to monitor its appearance and facilitate ap...Antibodies, Viral; Antigens, Viral; RNA, Viral2006
62 Liew, Michael; Erali, Maria; Page, Sam; Hillyard, David R.; Wittwer, Carl T.Hepatitis C genotyping by denaturing high-performance liquid chromatographyDetermination of the hepatitis C virus (HCV) genotype for infected patients increasingly has become accepted as the standard of care. Genotype assignment helps in assessing disease prognosis and assists in establishing the appropriate duration of treatment. The great genetic diversity of HCV, with 1...Base Sequence; 5' Untranslated Regions; Genotype; Hepacivirus; Molecular Sequence Data2004-01
63 Kriesel, John D.; Spruance, Spotswood L.; Daynes, Raymond A.Nucleic acid vaccine encoding gD2 protects mice from herpes simplex virus type 2 disease.Nucleic acid vaccinations with plasmids pWW65, containing the sequence for herpes simplex type 2 (HSV-2) gD2, and pRSVnt, lacking the gD sequence, were studied. Groups of mice were immunized with pWW65 alone, pWW65 plus 1,25-dihydroxyvitamin-D3 (D3), or pRSVnt. Clinical disease (vaginitis), serum an...Mice, Inbred BALB C; Plasmids; Viral Vaccines1996
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