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26 Erickson, J. Alan; Ashwood, Edward R.Evaluation of an enzyme-linked binding protein assay for hyaluronic acid and concentrations in hepatitis C infected patientsSerological hyaluronic acid (HA) has been proposed as a noninvasive alternative to liver biopsy for assessing the extent of liver fibrosis. Liver biopsy correctly identifies hepatic disease in about 65 to 75% of cases, being strongly dependent on the length of the biopsy obtained. Furthermore, hepat...Hyaluronic Acid Concentration; Corgenix HA assay2005-07-21
27 Erickson, J. Alan; Aldeen, William E.; Ashwood, Edward R.Evaluation of a fecal pancreatic elastase-1 enzyme-linked immunosorbent assay: assessment versus an established assay and implication in classifying pancreatic functionDisagreement continues regarding the two commercially available fecal pancreatic elastase-1 (PE-1) ELISAs and their respective capabilities to assess pancreatic function. Our objectives were to validate the newer PE-1 ELISA and evaluate the test against the previously established assay, to investiga...PE-1; pancreatic elastase-1; FDA; AMR; analytical measurement range; BioServ Diagnostics PE-1ELISA; ScheBo Biotech PE-1ELISA2005-09-25
28 Erickson, J. Alan; Smith, Jeffrey, J.; Bornhorst, Joshua A.; Ashwood, Edward R.Humananti-mouse antibody protected ELISA for the quantification of squamous cell carcinoma antigenSquamous cell carcinoma antigen (SCC) was first isolated from squamous cell carcinoma tissue of the uterine cervix and initially reported as TA-4. The antigen has been characterized as a glycoprotein with a molecular weight between 45,000 and 55,000 daltons. Studies suggest that SCC may function as ...SCC; Squamous Cell Carcinoma Antigen; BSA; PBS; Phosphate Buffered Saline; HAMA; Human Anti-mouse Antibodies; IIR; ImmunoglobulinInhibiting Reagent2005-12-09
29 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.; Comanor, Lorraine; Di Canzio, JamesEvaluation of the Versant ? HBV DNA 3.0 Assay (bDNA)+ and comparison with the Cobastm HBV Taqman Analyte Specific Reagent Assay.To evaluate the performance characteristics of the Versant ? HBV DNA 3.0 assay (bDNA)+ (Versant, Bayer Healthcare LLC, Tarrytown, NY). 2.) To compare the Versant assay to the COBAS HBV TaqMan Analyte Specific Reagent assay (TaqMan) (Roche Molecular Systems, Pleasanton, CA) using random and defined p...International Standard; HBV DNA Tests2005-05-07
30 Owen, William E.; Roberts, William L.Cross-reactivity of three recombinant insulin analogs with five commercial insulin immunoassaysSeveral new insulin analogs that are prepared with recombinant DNA technology are available for clinical use [for a recent review, see Ref. (1)]. These agents have altered pharmacokinetics compared with regular human insulin. Insulin aspart (Novo-LogTM; Novo Nordisk Pharmaceuticals) is homologous ...Insulin analogs; Insulin immunoassays; Cross-reactivity2004
31 Kriesel, John D.; Baringer, J R; Spruance, Spotswood L.Correlation between detection of herpes simplex virus in oral secretions by PCR and susceptibility to experimental UV radiation-induced herpes labialis.We examined the oral secretions of 25 patients for herpes simplex virus (HSV) at the time of and following experimental UV radiation (UVR). HSV was detected in one or more oral secretion specimens in 5 of 12 (42%) cases by cell culture and in 8 of 12 (67%) cases by PCR. On the day of UVR, HSV was de...Polymerase Chain Reaction; Ultraviolet Rays; Simplexvirus1994
32 Cloud, Joann L.; Carroll, Karen C.; Cohen, Samuel; Anderson, Clint M.; Woods, Gail L.Interpretive criteria for use of AccuProbe for identification ofRapid identification of Mycobacterium avium complex (MAC) is possible by use of AccuProbe (Gen-Probe, San Diego, Calif.). To evaluate the reliability of the MAC AccuProbe for testing 7H9 cultures inoculated with broth from MGIT cultures positive for acid-fast bacilli or growth on a s...Bacteria; Typing; Mycobacterium avium complex; MAC2005-03-03
33 Dwight, Zachary LawrenceuMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web applicationuMeltSM is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR products. The user defines an amplicon sequence and chooses a set of thermodynamic and experimental parameters that include nearest-neighbor stacking energies, loop entropy effects, cation (mono...2011
34 Roberts, William L.Specificity characteristics of 7 commercial creatinine measurement procedures by enzymatic and jaffe method principlesStandardized calibration does not change a creatinine measurement procedure?s susceptibility to potentially interfering substances.2012
35 Owen, William E.; Roberts, William L.Performance characteristics of an HPLC method for Urinary AlbuminMicroalbuminuria is a marker of early diabetic nephropathy and cardiovascular risk. Immunoassays for urinary albumin can underestimate the amount of intact albumin present in urine. The purpose of this study was to evaluate a new urinary albumin assay that employs size exclusion HPLC with UV detecti...MA; Microalbuminuria; HPLC2005-07-06
36 Owen, William E.; Roberts, William L.Effects of hemoglobin (Hb) E and HbD traits on measurements of glycated Hb (HbA1c) by 23 methods.Glycohemoglobin (GHB), reported as hemoglobin (Hb) A1c, is a marker of long-term glycemic control in patients with diabetes and is directly related to risk for diabetic complications. HbE and HbDare the second and fourth most common Hb variants worldwide. We investigated the accuracy of HbA1c measur...Glycohemoglobin; GHB; Glycated Hb; Glycemic control2008
37 Seipp, Michael; Williams, Jamie; Meadows, Cindy; Wittwer, Carl T.Multiplex solution genotyping without probes of the factor V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T and A1298C) mutations in one tube by single color high-resolution melting analysis [abstract]This abstract describes a study which concludes that genotyping by amplicon melting is a rapid, closed-tube method for genotyping without probes that can be multiplexed. The method was successfully applied to the four most common clotting factor mutations in a single tube. The simplicity of the meth...Genotyping; Amplicon Melting2005-06-22
38 Owen, William E.; Rockwood, Alan L.; Roberts, William L.; Meikle, A. WayneDevelopment and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroidsCongenital adrenal hyperplasia is a group of autosomal recessive disorders caused by a deficiency of 1 of 4 enzymes required for the synthesis of glucocorticoids, mineralocorticoids, and sex hormones. Analysis of 11-deoxycortisol (11DC), 17-hydroxyprogesterone (17OHP), 17-hydroxypregnenolone (17O...Congenital adrenal hyperplasia; Adrenal steroids; Tandem mass spectrometry assay2006
39 Hillyard, David R.Quantitative real time PCR assay for detecting BK virus in serum, plasma and urine.BK is a non-enveloped virus in the Polyomavirus family, closely related to SV40 and JC virus. Primary infection with BK generally occurs during childhood without specific symptoms, and is widespread in the population, with approximately 80% of adults infected globally. The virus remains latent in th...Quantitative Tests; Linearity Studies; Oligonucleotides2005-05-02
40 Jaskowski, Troy D.; Litwin, Christine M.; Hill, Harry R.Laboratory diagnosis of Inflammatory Bowel Disease (IBD)Study to compare the results obtained by two separate reference libraries for serological assays utlilized in the diagnosis and differentiation of Crohn's disease and ulcerative colitis, and to assess the clinical utility of the outer-membrane porin C (OmpC) IgA assay in IBD.IBD2005-06-17
41 Cloud, Joann L.; Meyer, Jay J.; Pounder, June I.; Woods, Gail L.Mycobacterium arupense sp. nov., a novel moderately growing nonchromogenic bacterium isolated from clinical specimensThis author manuscript discusses how several isolates of Mycobacterium species related to the M. terrae complex have been isolated from clinical samples. In the clinical microbiology laboratory, partial 16S rRNA gene sequencing (approximate first 500 base pairs) is often used to identify Mycobacteri...Mycobacterium arupense; Mycobacterium nonchromogenicum; Mycobacterium terrae; Mycobacterium triviale; MCRO 62005-11-22
42 Hymas, Weston C.; Hillyard, David R.Real time PCR assay for the detection of CMV using eclipse hybridization probesWe developed a qualitative real time PCR assay to detect CMV in patient samples, using a novel hybridization probe termed the Eclipse probe (Epoch Biosciences). These are single probes with a fluorescent molecule attached to the 3' end and a dark quencher on the 5' end. Also conjugated to the 5' end...Tissues Analysis; Elegans2004-08-11
43 Vaughn, Cecily P.; Crockett, David K.; Lim, Megan S.; Elenitoba-Johnson, Kojo S.J.Analytical characteristics of ICAT-LC-MS/MS for quantitative proteomics studiesRecent studies have employed isotope-coded affinity tags (ICAT) in concert with tandem mass spectrometry (MS/MS) to assess differential expression of proteins on a proteomic scale and perform unbiased quantitative comparisons between samples from different physiological or disease states. We sought ...isotope-coded affinity tags; ICAT; mass spectrometry; MS/MS; proteomic scale; FL2005-06-03
44 Margraf, Rebecca L.; Mao, Rong; Wittwer, Carl T.Masking techniques: masking selected sequence variation by incorporating mismatches into melting analysis probesHybridization probe melting analysis can be complicated by the presence of sequence variation (non-pathogenic polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Materials and Meth...probe hybridization; high-resolution melting analysis; masking; RET2005-10-21
45 Jaskowski, Troy D.Multiplex analysis of heterophil antibodies in patients with indeterminate HIV immunoassay resultsWe hypothesized that heterophil antibodies reactive with animal proteins used in blot preparation caused nonspecific staining (NSS) on HIV Western blot (WB) studies, causing indeterminate results. We analyzed samples showing NSS on HIV WB using a multiplexed immunoassay to simultaneously measur...Heterophil antibodies; Heterophil interference; Multiplex analysis; False-positive HIV enzyme-linked immunosorbent assay; ELISA2001
46 Pounder, June I.; Simmon, Keith E.; Barton, Claudia A.; Hohmann, Sheri L.Identification of nonsporulating molds by sequencing internal transcribed spacer regions with Virodec software and databaseFungal infections are increasing, particularly among immunocompromised hosts, and a rapid, accurate diagnosis is essential for the initiation of targeted antifungal therapy. Identification of fungi from culture requires the presence of reproductive structures, and the absence of spores can increase ...nonsporulating molds; genetic sequencing; Virodec; ITS I; ITS II2006-01-09
47 Jaskowski, Troy D.Polymerase chain reaction detection of Lyme disease: correlation with clinical manifestations and serologic responsesThe Author's have developed a simple, nested polymerase chain reaction (PCR) assay for amplification of an outer surface protein A (OspA) gene fragment of Borrelia burgdorferi using rapid temperature cycling and ethidium bromide detection on agarose gels, and applied it to the diagnosis of Lyme dis...PCR; Rapid cycle amplification1996
48 Eichwald, Ernst; Capecchi, Mario R.; Thomas, Kirk R.Mouse model for the delta F508 allele of cystic fibrosisThe most common cause of cystic fibrosis is a mutation that deletes phenylalanine 508 in cystic fibrosis transmembrane conductance regulator (CFTR). The delta F508 protein is misprocessed and degraded rather than traveling to the apical membrane. We used a novel strategy to introduce the delta F508 ...Digestive System; Disease Models, Animal; Electrolytes; Mice, Inbred C57BL1995-10
49 Stevenson, Jeff; Procter, Melinda; Hillyard, David R.Real time PCR assays for the detection of viral pathogens - are they the gold standard?Over the past decade, PCR testing for infectious agents in the clinical laboratory has begun to replace other methods, such as culture. PCR is often now referred to as the 'gold standard' in this field. Further evolution of this technology has seen the introduction of real time PCR, replacing tradit...Polymorphism; Target DNA2003-11-11
50 Konnick, Eric Q.; Erali, Maria; Ashwood, Edward R; Hillyard, David RComparison of the DIGENE HBV HYBRID CAPTURE II AND ROCHE COBAS Amplicor HBV Monitor Assays.Evaluate and compare the performance characteristics of the COBAS Amplicor HBV Monitor test (Roche Diagnostics Corp.) and the HBV Hybrid capture II (Digene Corporation) assay for the quantification of HBV BNA Levels.Quantitative Tests; Linearity Studies2001-08-19
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