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Creator | Title | Description | Subject | Date |
| 26 |
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Owen, William E.; Roberts, William L. | Cross-reactivity of three recombinant insulin analogs with five commercial insulin immunoassays | Several new insulin analogs that are prepared with recombinant DNA technology are available for clinical use [for a recent review, see Ref. (1)]. These agents have altered pharmacokinetics compared with regular human insulin. Insulin aspart (Novo-LogTM; Novo Nordisk Pharmaceuticals) is homologous ... | Insulin analogs; Insulin immunoassays; Cross-reactivity | 2004 |
| 27 |
 |
Kriesel, John D.; Baringer, J R; Spruance, Spotswood L. | Correlation between detection of herpes simplex virus in oral secretions by PCR and susceptibility to experimental UV radiation-induced herpes labialis. | We examined the oral secretions of 25 patients for herpes simplex virus (HSV) at the time of and following experimental UV radiation (UVR). HSV was detected in one or more oral secretion specimens in 5 of 12 (42%) cases by cell culture and in 8 of 12 (67%) cases by PCR. On the day of UVR, HSV was de... | Polymerase Chain Reaction; Ultraviolet Rays; Simplexvirus | 1994 |
| 28 |
 |
Cloud, Joann L.; Carroll, Karen C.; Cohen, Samuel; Anderson, Clint M.; Woods, Gail L. | Interpretive criteria for use of AccuProbe for identification of | Rapid identification of Mycobacterium avium complex (MAC) is possible by use of AccuProbe (Gen-Probe, San Diego, Calif.). To evaluate the reliability of the MAC AccuProbe for testing 7H9 cultures inoculated with broth from MGIT cultures positive for acid-fast bacilli or growth on a s... | Bacteria; Typing; Mycobacterium avium complex; MAC | 2005-03-03 |
| 29 |
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Dwight, Zachary Lawrence | uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application | uMeltSM is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR products. The user defines an amplicon sequence and chooses a set of thermodynamic and experimental parameters that include nearest-neighbor stacking energies, loop entropy effects, cation (mono... | | 2011 |
| 30 |
 |
Roberts, William L. | Specificity characteristics of 7 commercial creatinine measurement procedures by enzymatic and jaffe method principles | Standardized calibration does not change a creatinine measurement procedure?s susceptibility to potentially interfering substances. | | 2012 |
| 31 |
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Owen, William E.; Roberts, William L. | Performance characteristics of an HPLC method for Urinary Albumin | Microalbuminuria is a marker of early diabetic nephropathy and cardiovascular risk. Immunoassays for urinary albumin can underestimate the amount of intact albumin present in urine. The purpose of this study was to evaluate a new urinary albumin assay that employs size exclusion HPLC with UV detecti... | MA; Microalbuminuria; HPLC | 2005-07-06 |
| 32 |
 |
Owen, William E.; Roberts, William L. | Effects of hemoglobin (Hb) E and HbD traits on measurements of glycated Hb (HbA1c) by 23 methods. | Glycohemoglobin (GHB), reported as hemoglobin (Hb) A1c, is a marker of long-term glycemic control in patients with diabetes and is directly related to risk for diabetic complications. HbE and HbDare the second and fourth most common Hb variants worldwide. We investigated the accuracy of HbA1c measur... | Glycohemoglobin; GHB; Glycated Hb; Glycemic control | 2008 |
| 33 |
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Seipp, Michael; Williams, Jamie; Meadows, Cindy; Wittwer, Carl T. | Multiplex solution genotyping without probes of the factor V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T and A1298C) mutations in one tube by single color high-resolution melting analysis [abstract] | This abstract describes a study which concludes that genotyping by amplicon melting is a rapid, closed-tube method for genotyping without probes that can be multiplexed. The method was successfully applied to the four most common clotting factor mutations in a single tube. The simplicity of the meth... | Genotyping; Amplicon Melting | 2005-06-22 |
| 34 |
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Owen, William E.; Rockwood, Alan L.; Roberts, William L.; Meikle, A. Wayne | Development and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroids | Congenital adrenal hyperplasia is a group of autosomal recessive disorders caused by a deficiency of 1 of 4 enzymes required for the synthesis of glucocorticoids, mineralocorticoids, and sex hormones. Analysis of 11-deoxycortisol (11DC), 17-hydroxyprogesterone (17OHP), 17-hydroxypregnenolone (17O... | Congenital adrenal hyperplasia; Adrenal steroids; Tandem mass spectrometry assay | 2006 |
| 35 |
 |
Hillyard, David R. | Quantitative real time PCR assay for detecting BK virus in serum, plasma and urine. | BK is a non-enveloped virus in the Polyomavirus family, closely related to SV40 and JC virus. Primary infection with BK generally occurs during childhood without specific symptoms, and is widespread in the population, with approximately 80% of adults infected globally. The virus remains latent in th... | Quantitative Tests; Linearity Studies; Oligonucleotides | 2005-05-02 |
| 36 |
 |
Jaskowski, Troy D.; Litwin, Christine M.; Hill, Harry R. | Laboratory diagnosis of Inflammatory Bowel Disease (IBD) | Study to compare the results obtained by two separate reference libraries for serological assays utlilized in the diagnosis and differentiation of Crohn's disease and ulcerative colitis, and to assess the clinical utility of the outer-membrane porin C (OmpC) IgA assay in IBD. | IBD | 2005-06-17 |
| 37 |
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Cloud, Joann L.; Meyer, Jay J.; Pounder, June I.; Woods, Gail L. | Mycobacterium arupense sp. nov., a novel moderately growing nonchromogenic bacterium isolated from clinical specimens | This author manuscript discusses how several isolates of Mycobacterium species related to the M. terrae complex have been isolated from clinical samples. In the clinical microbiology laboratory, partial 16S rRNA gene sequencing (approximate first 500 base pairs) is often used to identify Mycobacteri... | Mycobacterium arupense; Mycobacterium nonchromogenicum; Mycobacterium terrae; Mycobacterium triviale; MCRO 6 | 2005-11-22 |
| 38 |
 |
Hymas, Weston C.; Hillyard, David R. | Real time PCR assay for the detection of CMV using eclipse hybridization probes | We developed a qualitative real time PCR assay to detect CMV in patient samples, using a novel hybridization probe termed the Eclipse probe (Epoch Biosciences). These are single probes with a fluorescent molecule attached to the 3' end and a dark quencher on the 5' end. Also conjugated to the 5' end... | Tissues Analysis; Elegans | 2004-08-11 |
| 39 |
 |
Vaughn, Cecily P.; Crockett, David K.; Lim, Megan S.; Elenitoba-Johnson, Kojo S.J. | Analytical characteristics of ICAT-LC-MS/MS for quantitative proteomics studies | Recent studies have employed isotope-coded affinity tags (ICAT) in concert with tandem mass spectrometry (MS/MS) to assess differential expression of proteins on a proteomic scale and perform unbiased quantitative comparisons between samples from different physiological or disease states. We sought ... | isotope-coded affinity tags; ICAT; mass spectrometry; MS/MS; proteomic scale; FL | 2005-06-03 |
| 40 |
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Wittwer, Carl T. | Genotyping accuracy of high-resolution DNA melting instruments | High-resolution DNA melting is a closed-tube method for genotyping and variant scanning that depends on the thermal stability of PCR-generated products. Instruments vary in thermal precision, sample format, melting rates, acquisition, and software. Instrument genotyping accuracy has not been assesse... | | 2014-01-01 |
| 41 |
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Margraf, Rebecca L.; Mao, Rong; Wittwer, Carl T. | Masking techniques: masking selected sequence variation by incorporating mismatches into melting analysis probes | Hybridization probe melting analysis can be complicated by the presence of sequence variation (non-pathogenic polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Materials and Meth... | probe hybridization; high-resolution melting analysis; masking; RET | 2005-10-21 |
| 42 |
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Jaskowski, Troy D. | Multiplex analysis of heterophil antibodies in patients with indeterminate HIV immunoassay results | We hypothesized that heterophil antibodies reactive with animal proteins used in blot preparation caused nonspecific staining (NSS) on HIV Western blot (WB) studies, causing indeterminate results. We analyzed samples showing NSS on HIV WB using a multiplexed immunoassay to simultaneously measur... | Heterophil antibodies; Heterophil interference; Multiplex analysis; False-positive HIV enzyme-linked immunosorbent assay; ELISA | 2001 |
| 43 |
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Pounder, June I.; Simmon, Keith E.; Barton, Claudia A.; Hohmann, Sheri L. | Identification of nonsporulating molds by sequencing internal transcribed spacer regions with Virodec software and database | Fungal infections are increasing, particularly among immunocompromised hosts, and a rapid, accurate diagnosis is essential for the initiation of targeted antifungal therapy. Identification of fungi from culture requires the presence of reproductive structures, and the absence of spores can increase ... | nonsporulating molds; genetic sequencing; Virodec; ITS I; ITS II | 2006-01-09 |
| 44 |
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Jaskowski, Troy D. | Polymerase chain reaction detection of Lyme disease: correlation with clinical manifestations and serologic responses | The Author's have developed a simple, nested polymerase chain reaction (PCR) assay for amplification of an outer surface protein A (OspA) gene fragment of Borrelia burgdorferi using rapid temperature cycling and ethidium bromide detection on agarose gels, and applied it to the diagnosis of Lyme dis... | PCR; Rapid cycle amplification | 1996 |
| 45 |
 |
Eichwald, Ernst; Capecchi, Mario R.; Thomas, Kirk R. | Mouse model for the delta F508 allele of cystic fibrosis | The most common cause of cystic fibrosis is a mutation that deletes phenylalanine 508 in cystic fibrosis transmembrane conductance regulator (CFTR). The delta F508 protein is misprocessed and degraded rather than traveling to the apical membrane. We used a novel strategy to introduce the delta F508 ... | Digestive System; Disease Models, Animal; Electrolytes; Mice, Inbred C57BL | 1995-10 |
| 46 |
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Stevenson, Jeff; Procter, Melinda; Hillyard, David R. | Real time PCR assays for the detection of viral pathogens - are they the gold standard? | Over the past decade, PCR testing for infectious agents in the clinical laboratory has begun to replace other methods, such as culture. PCR is often now referred to as the 'gold standard' in this field. Further evolution of this technology has seen the introduction of real time PCR, replacing tradit... | Polymorphism; Target DNA | 2003-11-11 |
| 47 |
 |
Konnick, Eric Q.; Erali, Maria; Ashwood, Edward R; Hillyard, David R | Comparison of the DIGENE HBV HYBRID CAPTURE II AND ROCHE COBAS Amplicor HBV Monitor Assays. | Evaluate and compare the performance characteristics of the COBAS Amplicor HBV Monitor test (Roche Diagnostics Corp.) and the HBV Hybrid capture II (Digene Corporation) assay for the quantification of HBV BNA Levels. | Quantitative Tests; Linearity Studies | 2001-08-19 |
| 48 |
 |
Normann, Richard A.; Chandler, John P. | Effects of calcium ions on L-type horizontal cells in the isolated turtle retina | A technique by which the retina can be isolated from the turtle eye is described. Scanning electron microscopy revealed morphological variability between preparations and also between regions of the same one. Large areas were often totally free of any pigment epithelial cells, yet contained a high p... | Retina; Calcium; Horizontal Cell; Turtle | 1990 |
| 49 |
 |
Jaskowski, Troy D. | Evaluation of a PCR probe capture assay for the detection of Toxoplasma gondii: incorporation of uracil N-glycosylase for contamination control | Toxoplasma gondii is a cyst-forming parasite of clinical relevance in humans primarily because of the neurologic abnormalities it can cause. In some clinical circumstances, it is desirable to detect the pathogen directly. We modified a commercially available Toxoplasma polymerase chain reaction... | PCR probe capture assay; Uracil N-glycosylase; UNG | 2000 |
| 50 |
 |
Pounder, June I.; Voelkerding, Karl V.; Storts, D.; Anderson, Clint M. | Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysis | Differentiation of members within Mycobacterium tuberculosis complex (MTC) is essential for patient management and infection control. MTC is comprised of M. tuberculosis (Mtb), M. bovis (Mb), M. bovis BCG (vaccine strain; Mbcg), M. africanum (Ma), and M. microti (Mm). Because MTC have highly similar... | MTC; species differentiation; Mycobacterium bovis BCG; Mycobacterium africanum; Mycobacterium microtic; PCR | 2006-01-09 |
