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26 Evaluation of a PCR probe capture assay for the detection of Toxoplasma gondii: incorporation of uracil N-glycosylase for contamination controlToxoplasma gondii is a cyst-forming parasite of clinical relevance in humans primarily because of the neurologic abnormalities it can cause. In some clinical circumstances, it is desirable to detect the pathogen directly. We modified a commercially available Toxoplasma polymerase chain reaction...PCR probe capture assay; Uracil N-glycosylase; UNG2000
27 Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysisDifferentiation of members within Mycobacterium tuberculosis complex (MTC) is essential for patient management and infection control. MTC is comprised of M. tuberculosis (Mtb), M. bovis (Mb), M. bovis BCG (vaccine strain; Mbcg), M. africanum (Ma), and M. microti (Mm). Because MTC have highly similar...MTC; species differentiation; Mycobacterium bovis BCG; Mycobacterium africanum; Mycobacterium microtic; PCR2006-01-09
28 Mass spectrometry for steroids, immunosuppressants, and other clinical applicationsPresentation slides on matching mass spectrometry technology to clinical applications.Immunosuppressants2005-07-26
29 Performance characteristics of eight estradiol immunoassaysMeasurement of estradiol is useful in assisted reproduction, evaluation of infertility, menopause, and male feminization. The analytic performance of 8 estradiol immunoassays was evaluated. The imprecision and accuracy of the Access, ADVIA Centaur, ARCHITECT i2000, AutoDELFIA, Elecsys 2010, IMMULI...Estradiol immunoassays2004
30 Differentiation of mycobacterium chelonae and M. abscessus using SmartCycler PCR and MGB eclipse probesThis poster discusses how the use of partial 16S rDNA sequencing (first one-third of the gene) for the identification of Mycobacterium species from cultured isolates has become recognized as a very accurate method for identification. The distinction between M. chelonae and M. abscessus, however, is ...MGB Eclipse System; SmartCycler PCR; Mycobacterium abscessus2005-09-05
31 Performance characteristics of seven automated CA125 assaysAuthor manuscript. Cancer antigen 125 (CA125) is a high molecular mass glycoprotien, which is used as tumor marker to monitor disease progression, response to therapy and in early detection of recurrence after treatment for ovarian cancer. The Access 2, ADVIA Centaur, ARCHITECT i2000, AxSYM, Elecsys...CA125 assays2005-08-29
32 Evaluation of an enzyme-linked binding protein assay for hyaluronic acid and concentrations in hepatitis C infected patientsSerological hyaluronic acid (HA) has been proposed as a noninvasive alternative to liver biopsy for assessing the extent of liver fibrosis. Liver biopsy correctly identifies hepatic disease in about 65 to 75% of cases, being strongly dependent on the length of the biopsy obtained. Furthermore, hepat...Hyaluronic Acid Concentration; Corgenix HA assay2005-07-21
33 Evaluation of a fecal pancreatic elastase-1 enzyme-linked immunosorbent assay: assessment versus an established assay and implication in classifying pancreatic functionDisagreement continues regarding the two commercially available fecal pancreatic elastase-1 (PE-1) ELISAs and their respective capabilities to assess pancreatic function. Our objectives were to validate the newer PE-1 ELISA and evaluate the test against the previously established assay, to investiga...PE-1; pancreatic elastase-1; FDA; AMR; analytical measurement range; BioServ Diagnostics PE-1ELISA; ScheBo Biotech PE-1ELISA2005-09-25
34 Humananti-mouse antibody protected ELISA for the quantification of squamous cell carcinoma antigenSquamous cell carcinoma antigen (SCC) was first isolated from squamous cell carcinoma tissue of the uterine cervix and initially reported as TA-4. The antigen has been characterized as a glycoprotein with a molecular weight between 45,000 and 55,000 daltons. Studies suggest that SCC may function as ...SCC; Squamous Cell Carcinoma Antigen; BSA; PBS; Phosphate Buffered Saline; HAMA; Human Anti-mouse Antibodies; IIR; ImmunoglobulinInhibiting Reagent2005-12-09
35 Comparison of four enzyme immunoassays with a western blot assay for the determination of type-specific antibodies to herpes simplex virusMost current enzyme immunoassays (EIAs) differentiate inadequately between types 1 and 2 herpes simplex virus (HSV) antibodies since significant crossreactivity exists. We compared 4 IgG type-specific EIAs using a Western blot assay for resolution of discrepant results. The Diamedix had sensitivit...Enzyme immunoassays; Western blot assay; Type-specific antibodies2001
36 Alveolar rhabdomyosarcomas in conditional Pax3:Fkhr mice: cooperativity of Ink4a/ARF and Trp53 loss of function.Alveolar rhabdomyosarcoma is an aggressive childhood muscle cancer for which outcomes are poor when the disease is advanced. Although well-developed mouse models exist for embryonal and pleomorphic rhabdomyosarcomas, neither a spontaneous nor a transgenic mouse model of alveolar rhabdomyosarcoma has...Cell Differentiation; Forkhead Transcription Factors; Myogenic Regulatory Factors2004-11-01
37 Do dentists prescribe narcotics excessively?Dealing with pain is an inevitable sequela to dental treatment. Although several drug regimens primarily involving narcotics have been used in the past, availability of nonsteroidal anti-inflammatory drugs (NSAIDs) has increased recently. A study was conducted to analyze dental prescribing patterns ...Analgesics, Opioid; Anti-Inflammatory Agents; Dentist's Practice Patterns; Pain, Postoperative1996-07-01
38 Laboratory driven, lean-to-adaptive prototyping in Parallel for Web software project identification and application development in health science researchClinical research laboratories, bioinformatics core facilities, and health science organizations often rely on heavy planning based software development models to propose, build, and distribute software as a consumable product. Projects in non-agile software life cycles tend to have rigid ?plan-desi...2012
39 Development and validation of an automated thawing and mixing workcellWorking toward a goal of total laboratory automation, we are automating manual activities in our highest volume laboratory section. Because half of all specimens arriving in this laboratory section are frozen, we began by developing an automated workcell for thawing frozen specimens and mixing the t...Automated workcell; Laboratory automation2007
40 Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) and COBAS HCV TaqMan ASR Assays using the Vidiera NSP sample preparation instrument.The Vidiera Nsp nucleic sample preparation instrument (Vidiera,Beckman-Coulter Inc., Fullerton CA) was evaluated using the COBAS HBV TaqMan Analyte Specific Reagent (ASR. Roche Molecular Diagnostics [RMD], Indianapolis, IN) and COBAS HCV TaqMan ASR (RMD) assays using clinical samples and HCV Armored...Quantitative Tests; Linearity Studies2006-05-05
41 Effects of hemoglobin C and S traits on the results of 14 commercial glycated hemoglobin assaysGlycated hemoglobin is widely used in the management of diabetes mellitus. At least 300,000 Americans with diabetes mellitus have the hemoglobin (Hb) C or S trait. The accuracy of HbA1c methods can be adversely affected by the presence of these traits. We evaluated the effects of HbC and HbS traits ...Glycated hemoglobin assays; Hemoglobin C trait; Hemoglobin S trait2008
42 Assessment of the utility of viral culture of cerebrospinal fluidNucleic acid amplification testing is the preferred method to detect enteroviruses and Herpesviridae in cerebrospinal fluid, but clinicians still request viral culture. Review of 22,394 viral cultures of cerebrospinal fluid samples found that <0.1% recovered nonenterovirus, non-Herpesviridae species...Enterovirus Infections; Herpesviridae Infections; Virus Cultivation2006-12-15
43 Cross-reactivity of three recombinant insulin analogs with five commercial insulin immunoassaysSeveral new insulin analogs that are prepared with recombinant DNA technology are available for clinical use [for a recent review, see Ref. (1)]. These agents have altered pharmacokinetics compared with regular human insulin. Insulin aspart (Novo-LogTM; Novo Nordisk Pharmaceuticals) is homologous ...Insulin analogs; Insulin immunoassays; Cross-reactivity2004
44 Correlation between detection of herpes simplex virus in oral secretions by PCR and susceptibility to experimental UV radiation-induced herpes labialis.We examined the oral secretions of 25 patients for herpes simplex virus (HSV) at the time of and following experimental UV radiation (UVR). HSV was detected in one or more oral secretion specimens in 5 of 12 (42%) cases by cell culture and in 8 of 12 (67%) cases by PCR. On the day of UVR, HSV was de...Polymerase Chain Reaction; Ultraviolet Rays; Simplexvirus1994
45 Real time PCR assay for the detection of CMV using eclipse hybridization probesWe developed a qualitative real time PCR assay to detect CMV in patient samples, using a novel hybridization probe termed the Eclipse probe (Epoch Biosciences). These are single probes with a fluorescent molecule attached to the 3' end and a dark quencher on the 5' end. Also conjugated to the 5' end...Tissues Analysis; Elegans2004-08-11
46 Analytical characteristics of ICAT-LC-MS/MS for quantitative proteomics studiesRecent studies have employed isotope-coded affinity tags (ICAT) in concert with tandem mass spectrometry (MS/MS) to assess differential expression of proteins on a proteomic scale and perform unbiased quantitative comparisons between samples from different physiological or disease states. We sought ...isotope-coded affinity tags; ICAT; mass spectrometry; MS/MS; proteomic scale; FL2005-06-03
47 Nucleic acid vaccine encoding gD2 protects mice from herpes simplex virus type 2 disease.Nucleic acid vaccinations with plasmids pWW65, containing the sequence for herpes simplex type 2 (HSV-2) gD2, and pRSVnt, lacking the gD sequence, were studied. Groups of mice were immunized with pWW65 alone, pWW65 plus 1,25-dihydroxyvitamin-D3 (D3), or pRSVnt. Clinical disease (vaginitis), serum an...Mice, Inbred BALB C; Plasmids; Viral Vaccines1996
48 Multiplex analysis of heterophil antibodies in patients with indeterminate HIV immunoassay resultsWe hypothesized that heterophil antibodies reactive with animal proteins used in blot preparation caused nonspecific staining (NSS) on HIV Western blot (WB) studies, causing indeterminate results. We analyzed samples showing NSS on HIV WB using a multiplexed immunoassay to simultaneously measur...Heterophil antibodies; Heterophil interference; Multiplex analysis; False-positive HIV enzyme-linked immunosorbent assay; ELISA2001
49 Comparison of five automated serum and whole blood folate assaysSerum and whole blood folate measurements are used to establish folate deficiency. Most methods used in clinical laboratories are automated, nonisotopic methods that use folate-binding protein. Linearity, imprecision, and method comparison studies, including serum and whole blood hemolysates, wer...Folate assays; Serum folate; Whole blood folate; Automated assays2003
50 Identification of nonsporulating molds by sequencing internal transcribed spacer regions with Virodec software and databaseFungal infections are increasing, particularly among immunocompromised hosts, and a rapid, accurate diagnosis is essential for the initiation of targeted antifungal therapy. Identification of fungi from culture requires the presence of reproductive structures, and the absence of spores can increase ...nonsporulating molds; genetic sequencing; Virodec; ITS I; ITS II2006-01-09
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