Filters: Department: "Oncological Sciences" School Or College: "School of Medicine" Collection: "ir_uspace"
| Creator | Title | Description | Subject | Date | ||
|---|---|---|---|---|---|---|
| 26 |
 |
Capecchi, Mario R.; Mansour, Suzanne L.; Thomas, Kirk R. | Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. | Gene targeting--homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences--in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive nd negative selection procedure that enriches 2,00... | Animals; Clone Cells; Restriction Mapping; Stem Cells | 1988-11-24 |
| 27 |
 |
Capecchi, Mario R. | Gene targeting: tapping the cellular telephone. | Gene targeting means that we now have the potential to generate mice of virtually any desired genotype. In the first instance, standards recombinant DNA technology is used to alter a cloned DNA sequence of a chosen locus; the modified potent stem cell derived from a mouse embryo, and homologous reco... | Animals; Blotting, Southern; Bone and Bones; Gene Targeting; Genotype; Immunochemistry; In Situ Hybridization; Mice | 1990-03-08 |
| 28 |
 |
Thomas, Kirk R.; Capecchi, Mario R. | Targeted disruption of the murine int-1 proto-oncogene resulting in severe abnormalities in midbrain and cerebellar development. | The int-1 proto-oncogene was first identified as a gene activated in virally induced mouse mammary tumours. Expression studies, however, suggest that the normal function of this gene may be in spermatogenesis and in the development of the central nervous system. Genes sharing sequence similarity wit... | Animals; Blastocyst; Cell Line; Chimera; DNA; Female; Genotype; Heterozygote; Homozygote; Male; Mice, Inbred C57BL; Motor Activity | 1990-08-30 |
| 29 |
 |
Mansour, Suzanne L.; Thomas, Kirk R.; Capecchi, Mario R. | Introduction of a lacZ reporter gene into the mouse int-2 locus by homologous recombination. | We demonstrate that the frequency of gene targeting is unaffected by the length of nonhomologous DNA transferred to a target chromosomal sequence. A result of this finding is that a much wider spectrum of designed genomic alterations is now feasible. As a first application, we inserted a 5.4-kilobas... | Blotting, Southern; Cell Differentiation; Fluorescent Antibody Technique; Restriction Mapping | 1990-10 |
| 30 |
 |
Capecchi, Mario R. | Gene targeting. How efficient can you get? | With targeting in Leishmania and Trypanosoma, there are now greater incentives to develop the technology in organisms of intermediate complexity such as Caenorhabditis elegans and Drosophila. The ratio of homologous to nonhomologus recombination in these organisms might prove to be greater than in m... | Animals; DNA Mutational Analysis; Recombination, Genetic | 1990-11-08 |
| 31 |
 |
Capecchi, Mario R. | Regionally restricted developmental defects resulting from targeted disruption of the mouse homeobox gene hox-1.5. | Gene targeting in mouse embryo-derived stem cells has been used to disrupt the homeobox gene hox-1.5. Mice heterozygous at the hox-1.5 locus appear normal, whereas hox-1.5-/hox-1.5- mice die at or shortly after birth. These homozygotes are athymic, aparathyroid, have reduced thyroid and submaxillary... | Abnormalities, Multiple; Animals, Newborn; Arteries; Bone and Bones; Cartilage; Esophagus; Gene Expression; Heart Defects, Congenital; Organ Specificity; Parathyroid Glands; Pharynx; Thymus Gland; Thyroid Gland; Trachea | 1991-04-11 |
| 32 |
 |
Capecchi, Mario R. | Lessons from angiotensin-converting enzyme-deficient mice. | Mice which lack ACE have low systolic blood pressure, reduced male fertility and a renal abnormality characterized by medullary hypoplasia and the inability to concentrate urine. The diverse phenotypes caused by inactivation of a single gene emphasize the many functional roles of ACE and the renin-q... | Blood Pressure; Cell Line;Fertility; Kidney; Testis | 1991-11-01 |
| 33 |
 |
Capecchi, Mario R. | Creating mice with targeted disruptions in protooncogenes and homeobox genes, NIH Director's Lecture given May 21, 1992 | This is an audio transcript of a 1 hour, 2 minutes and 12 seconds lecture given at the National Institutes of Health by Mario Capecchi on 1992/05/21. Beginning with a short summary of the mechanics of gene transfer and the ability to create germline chimera with mutations that become hereditary wit... | Transgenic mice; Gene targeting; Genetic engineering; Molecular genetics; Mutagenesis; Homeobox genes; Histology - Pathological; Gene expression; Gene regulation; Genotype; Phenotype | 1992 |
| 34 |
|
KSL News clip of Mario Capecchi receiving the National Medal of Science for 1991 | This 1 minute, 44 seconds news clip from Salt Lake City's KSLTV, Channel 5 shows Mario Capecchi receiving the National Medal of Science for 1991 from President George W. Bush at the June 13, 2002 awards ceremony. The medal was presented for Capecchi's work in genetics and his development of the pro... | Transgenic mice; Gene targeting; Capecchi, Mario R.; Science - Awards - United States; Television broadcasting of news | 1992-06 | |
| 35 |
 |
Thomas, Kirk R.; Capecchi, Mario R. | High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors. | Mutations were targeted to the Hprt locus in murine embryonic stem cells by using sequence replacement vectors. When the vector was designed such that the mutated sequences were flanked on both sides by several kilobases of DNA homologous to the target locus, replacement of chromosomal sequences wit... | Drug Resistance; Gene Rearrangement; Gentamicins | 1992-07 |
| 36 |
 |
Capecchi, Mario R. | Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus. | Mutations were targeted to the Hprt locus of mouse embryo-derived stem cells by using 22 different sequence replacement and sequence insertion vectors. The targeting frequency was examined at two sites within the Hprt locus as a function of the extent of homology between the targeting vector and the... | Cell Line; DNA Transposable Elements; Embryo; Exons; Mice | 1992-08-12 |
| 37 |
 |
Thomas, Kirk R.; Capecchi, Mario R. | Location of crossovers during gene targeting with insertion and replacement vectors. | Gene targeting was used to introduce nonselectable genetic changes into chromosomal loci in mouse embryo-derived stem cells. The nonselectable markers were linked to a selectable marker in both insertion- and replacement-type vectors, and the transfer of the two elements to the Hprt locus was assaye... | Genetic Vectors; Molecular Sequence Data; Restriction Mapping | 1993-04 |
| 38 |
|
Local television news clips for June 14, 1994 featuring Mario Capecchi | This is a compilation of news clips from Salt Lake City's four major commercial television stations (Channels 2, 4, 5, and 13) lasting a total of 2 minutes, 59 seconds. The topic is Capecchi's winning of the General Motors Corporation's Alfred P. Sloan Jr. Prize for Outstanding Basic Science Contrib... | Transgenic mice; Gene targeting; Cancer - Research - Awards; Capecchi, Mario R.; Science - Awards - United States; Television broadcasting of news | 1994-06-14 | |
| 39 |
 |
Capecchi, Mario R. | Mice with targeted disruptions in the paralogous genes hoxa-3 and hoxd-3 reveal synergistic interactions. | The Hox genes encode transcription factors which mediate the formation of the mammalian body plan along the anteroposterior and appendicular axes. Paralogous Hox genes within the separate linkage groups are closely related with respect to DNA sequence and expression, suggesting that they could have ... | Animals; Atlas; Homozygote; Mice; Models, Genetic | 1994-07-28 |
| 40 |
 |
Capecchi, Mario R. | Targeted disruption of the even-skipped gene, evx1, causes early postimplantation lethality of the mouse conceptus. | Implantation within the mammalian uterus elicits dramatic changes in the growth, differentiation, and morphogenesis of the conceptus. This process is interrupted in mice carrying a targeted disruption of the murine evx1 gene, a homolog of the Drosophila even-skipped (eve) gene. Upon implantation, pr... | Extraembryonic; Homozygotes; Drosophila | 1994-08-15 |
| 41 |
 |
Capecchi, Mario R. | Genetic interaction between hoxb-5 and hoxb-6 is revealed by nonallelic noncomplementation. | hoxb-5 and hoxb-6 are adjacent genes in the mouse HoxB locus and are members of the homeotic transcription factor complex that governs establishment of the mammalian body plan. To determine the roles of these genes during development, we generated mice with a targeted disruption in each gene. Three ... | Alleles; CCAAT-Enhancer-Binding Proteins; Cervical Vertebrae; DNA-Binding Proteins; Forelimb; Genetic Complementation Test | 1995-01-01 |
| 42 |
 |
Capecchi, Mario R. | Introduction: the molecular genetic analysis of mouse development | This paper is an introduction of seven different papers presented in "Seminars in developmental biology" on Molecular Genetic Analysis of Mouse Development . The first paper, by Janet Rossant, describes very early mouse development. The second paper, by Frank Conlon and Rosa Beddington provide an i... | Embryo Culture Techniques; Genes | 1995-04 |
| 43 |
 |
Eichwald, Ernst; Capecchi, Mario R.; Thomas, Kirk R. | Mouse model for the delta F508 allele of cystic fibrosis | The most common cause of cystic fibrosis is a mutation that deletes phenylalanine 508 in cystic fibrosis transmembrane conductance regulator (CFTR). The delta F508 protein is misprocessed and degraded rather than traveling to the apical membrane. We used a novel strategy to introduce the delta F508 ... | Digestive System; Disease Models, Animal; Electrolytes; Mice, Inbred C57BL | 1995-10 |
| 44 |
 |
Capecchi, Mario R. | Hox genes and mammalian development | We have examined the interactions of Hox genes in forming a cervical vertebrae, hindbrain, and limbs. In each case, it is apparent that individual Hox genes are performing individual functions but that more profound roles are apparent when they act in combination with others Hox genes. The observed ... | Drosophila; Gene Expression Regulation, Developmental; Homozygote | 1997 |
| 45 |
|
Franklin Institute award dinner and ceremony held May 1, 1997 | This is a 1 hour, 42 minutes, 53 seconds video of the 1997 Franklin Institute Awards Banquet and Ceremony. The awards included a summary of each winner's achievements, the presentation of the award, and an acceptance speech. The award winners included Ralph L. Brinster, the Bower Award and Prize f... | Transgenic mice; Gene targeting; Genetic engineering; Molecular genetics; Mutagenesis; Homeobox genes; Histology - Pathological; Gene expression; Gene regulation; Genotype; Phenotype | 1997 | |
| 46 |
 |
Capecchi, Mario R. | Targeted mutations in hoxa-9 and hoxb-9 reveal synergistic interactions. | Mice were generated with a targeted disruption of the homeobox-containing gene hoxb-9. Mice homozygous for this mutation show defects in the development of the first and second ribs. In most cases the first and second ribs are fused near the point at which the first and second pairs of ribs normally... | Embryonic and Fetal Development; In Situ Hybridization; Mice, Knockout | 1997-01-15 |
| 47 |
 |
Capecchi, Mario R. | Critical role of tissue angiotensin-converting enzyme as revealed by gene targeting in mice | Angiotensin-converting enzyme (ACE) generates the vasoconstrictor angiotensin II, which plays a critical role in maintenance of blood pressure in mammals. Although significant ACE activity is found in plasma, the majority of the enzyme is bound to tissues such as the vascular endothelium. We used ta... | Blood pressure; ACE activity; Enzyme | 1997-03-06 |
| 48 |
 |
Capecchi, Mario R. | Cleft palate in mice with a targeted mutation in the gamma-aminobutyric acid-producing enzyme glutamic acid decarboxylase 67. | The functions of neurotransmitters in fetal development are poorly understood. Genetic observations have suggested a role for the inhibitory amino acid neurotransmitter gamma-aminobutyric acid (GABA) in the normal development of the mouse palate. Mice homozygous for mutations in the beta-3 GABAA rec... | Animals, Newborn; Crosses, Genetic | 1997-10-14 |
| 49 |
|
KUTV news featurette on cloning, molecular genetics, and disease aired November 9, 1997 | This 3 minutes, 1 second video clip from Salt Lake City's KUTV, Channel 2 features a short interview with Mario Capecchi concerning cloning and its use in molecular genetics and the study of human diseases. | Gene targeting; Genetic engineering; Molecular genetics; Cancer Research; Capecchi, Mario R.; Science - Moral and ethical aspects; Human chromosome abnormalities - Diagnosis; Television feature stories | 1997-11-09 | |
| 50 |
 |
Capecchi, Mario R. | Hox group 3 paralogous genes act synergistically in the formation of somitic and neural crest-derived structures. | Hox genes encode transcription factors that are used to regionalize the mammalian embryo. Analysis of mice carrying targeted mutations in individual and multiple Hox genes is beginning to reveal a complex network of interactions among these closely related genes which is responsible for directing th... | Abnormalities, Multiple; Gene Targeting; Glossopharyngeal Nerve; Mice, Knockout; Morphogenesis | 1997-12-15 |