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CreatorTitleDescriptionSubjectDate
1 Dwight, Zachary LawrenceHeterozygote PCR product melting curve predictionMelting curve prediction of PCR products is limited to perfectly complementary strands. Multiple domains are calculated by recursive nearest-neighbor thermodynamics. However, the melting curve of an amplicon containing a heterozygous single nucleotide variant (SNV) after PCR is the composite of four...2014-01-01
2 Cloud, Joann L.; Meyer, Jay J.; Pounder, June I.; Woods, Gail L.Mycobacterium arupense sp. nov., a novel moderately growing nonchromogenic bacterium isolated from clinical specimensThis author manuscript discusses how several isolates of Mycobacterium species related to the M. terrae complex have been isolated from clinical samples. In the clinical microbiology laboratory, partial 16S rRNA gene sequencing (approximate first 500 base pairs) is often used to identify Mycobacteri...Mycobacterium arupense; Mycobacterium nonchromogenicum; Mycobacterium terrae; Mycobacterium triviale; MCRO 62005-11-22
3 Jaskowski, Troy D.; Litwin, Christine M.; Hill, Harry R.Laboratory diagnosis of Inflammatory Bowel Disease (IBD)Study to compare the results obtained by two separate reference libraries for serological assays utlilized in the diagnosis and differentiation of Crohn's disease and ulcerative colitis, and to assess the clinical utility of the outer-membrane porin C (OmpC) IgA assay in IBD.IBD2005-06-17
4 Slev, Patricia R.; Rawlins, Mindy, L.; Roberts, William L.Performance characteristics of seven automated CA 15-3 methodsPoster describing studies to evaluate seven automated methods for the following parameters: limit of detection, linearity, imprecision, reference intervals and method comparison with the ADVIA Centaur as the comparison method to detect CA 15-3.CA 15-3 Method2005-07-13
5 Konnick, Eric Q.; Erali, Maria; Ashwood, Edward R; Hillyard, David RComparison of the DIGENE HBV HYBRID CAPTURE II AND ROCHE COBAS Amplicor HBV Monitor Assays.Evaluate and compare the performance characteristics of the COBAS Amplicor HBV Monitor test (Roche Diagnostics Corp.) and the HBV Hybrid capture II (Digene Corporation) assay for the quantification of HBV BNA Levels.Quantitative Tests; Linearity Studies2001-08-19
6 Cloud, Joann L.; Carroll, Karen C.; Cohen, Samuel; Anderson, Clint M.; Woods, Gail L.Interpretive criteria for use of AccuProbe for identification ofRapid identification of Mycobacterium avium complex (MAC) is possible by use of AccuProbe (Gen-Probe, San Diego, Calif.). To evaluate the reliability of the MAC AccuProbe for testing 7H9 cultures inoculated with broth from MGIT cultures positive for acid-fast bacilli or growth on a s...Bacteria; Typing; Mycobacterium avium complex; MAC2005-03-03
7 Dwight, Zachary LawrenceuMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web applicationuMeltSM is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR products. The user defines an amplicon sequence and chooses a set of thermodynamic and experimental parameters that include nearest-neighbor stacking energies, loop entropy effects, cation (mono...2011
8 Petti, Cathy A.Role of the laboratory in diagnosis of influenza during seasonal epidemics and potential pandemicsLaboratory diagnosis of influenza is critical to its treatment and surveillance. With the emergence of novel and highly pathogenic avian influenza viruses, the role of the laboratory has been further extended to include isolation and subtyping of the virus to monitor its appearance and facilitate ap...Antibodies, Viral; Antigens, Viral; RNA, Viral2006
9 Konnick, Eric Q.; Erali, Maria; Ashwood, Edward R.; Hillyard, David R.Comparison of the National Genetics Institute (NGI) HCV Superquant and Roche COBAS Amplicor HCV Monitor, Version 2.0 AssaysChronic Hepatitis C infection is currently recognized as an important health care problem. It is estimated that nearly 4 million Americans are infected, 15 to 20 percent of whom will eventually develop Cirrhosis. Current treatments for Hepatitis C virus (HCV) are limited to alfa-interferon alone or ...Cirrhosis; Patients2000-04-22
10 Litwin, Christine M.Anti-tuberculosis IgG antibodies as a marker of active mycobacterium tuberculosis diseaseAnti-Mycobacterium tuberculosis IgG antibodies may aid in the diagnosis of active M. tuberculosis disease. We studied whether anti-M. tuberculosis IgG antibodies are elevated in active M. tuberculosis disease and assessed factors contributing to false positive and negative results. A retrospective s...2012-01-01
11 Hymas, Weston C.; Hillyard, David R.Description and validation of a novel real-time RT-PCR enterovirus assayEnteroviruses are a leading cause of aseptic meningitis in adult and pediatric populations. We describe the development of a real-time RT-PCR assay that amplifies a small target in the 5' nontranslated region upstream of the classical Rotbart enterovirus amplicon. The assay includes an RNA internal ...RT-PCR enterovirus assay; Rotbart enterovirus amplicon; Aseptic meningitis2008
12 Hymas, Weston C.Ultrasonic enhancement of antibiotic action on several species of bacteriaThe effect of the antibiotics gentamicin, streptomycin, kanamycin, tetracycline, and ampicillin on planktonic cultures of Enterobacter aerogenes, Serratia marcescens, Salmonella derby, Streptococcus mitis, and Staphylococcus epidermidis with and without an application of 70 kHz ultrasound was studi...Enterobacter aerogenes; Serratia marcescens; Salmonella derby; Streptococcus mitis; Staphylococcus epidermidis; Planktonic cultures; Gentamicin; Streptomycin; Kanamycin; Tetracycline; Ampicillin; Ultrasonic enhancement; Bioacoustic effect1998
13 Owen, William E.; Roberts, William L.Comparison of five automated serum and whole blood folate assaysSerum and whole blood folate measurements are used to establish folate deficiency. Most methods used in clinical laboratories are automated, nonisotopic methods that use folate-binding protein. Linearity, imprecision, and method comparison studies, including serum and whole blood hemolysates, wer...Folate assays; Serum folate; Whole blood folate; Automated assays2003
14 Pounder, June I.; Simmon, Keith E.; Barton, Claudia A.; Hohmann, Sheri L.Identification of nonsporulating molds by sequencing internal transcribed spacer regions with Virodec software and databaseFungal infections are increasing, particularly among immunocompromised hosts, and a rapid, accurate diagnosis is essential for the initiation of targeted antifungal therapy. Identification of fungi from culture requires the presence of reproductive structures, and the absence of spores can increase ...nonsporulating molds; genetic sequencing; Virodec; ITS I; ITS II2006-01-09
15 Jaskowski, Troy D.Polymerase chain reaction detection of Lyme disease: correlation with clinical manifestations and serologic responsesThe Author's have developed a simple, nested polymerase chain reaction (PCR) assay for amplification of an outer surface protein A (OspA) gene fragment of Borrelia burgdorferi using rapid temperature cycling and ethidium bromide detection on agarose gels, and applied it to the diagnosis of Lyme dis...PCR; Rapid cycle amplification1996
16 Eichwald, Ernst; Capecchi, Mario R.; Thomas, Kirk R.Mouse model for the delta F508 allele of cystic fibrosisThe most common cause of cystic fibrosis is a mutation that deletes phenylalanine 508 in cystic fibrosis transmembrane conductance regulator (CFTR). The delta F508 protein is misprocessed and degraded rather than traveling to the apical membrane. We used a novel strategy to introduce the delta F508 ...Digestive System; Disease Models, Animal; Electrolytes; Mice, Inbred C57BL1995-10
17 Stevenson, Jeff; Procter, Melinda; Hillyard, David R.Real time PCR assays for the detection of viral pathogens - are they the gold standard?Over the past decade, PCR testing for infectious agents in the clinical laboratory has begun to replace other methods, such as culture. PCR is often now referred to as the 'gold standard' in this field. Further evolution of this technology has seen the introduction of real time PCR, replacing tradit...Polymorphism; Target DNA2003-11-11
18 Jaskowski, Troy D.Screening for antinuclear antibodies by enzyme immunoassayIndirect fluorescent antibody (IFA) is the most widely used method in clinical laboratories to screen for autoantibodies against a wide variety of nuclear antigens. Recently, a number of antinuclear antibody (ANA) enzyme immunoassay (EIA) screens have become commercially available and claim to be an...Antinuclear antibodies; Enzyme immunoassay1996
19 Hansen, Mark S.; Coffin, Cheryl M.; Capecchi, Mario R.Pax3:Fkhr interferes with embryonic Pax3 and Pax7 function: implications for alveolar rhabdomyosarcoma cell of origin.To investigate the role of the translocation-associated gene Pax3:Fkhr in alveolar rhabdomyosarcomas, we generated a Cre-mediated conditional knock-in of Pax3:Fkhr into the mouse Pax3 locus. Exploring embryonic tumor cell origins, we replaced a Pax3 allele with Pax3:Fkhr throughout its expression do...Neuroprogenitor; Embryogenesis2004-11-01
20 Singleton, J. Robinson; Smith, A. GordonMicrovascular complications of impaired glucose tolerance.Impaired glucose tolerance (IGT) serves as a marker for the state of insulin resistance and predicts both large- and small-vessel vascular complications, independent of a patient's progression to diabetes. Patients with IGT are at significantly increased risk for death and morbidity due to myocardia...Microcirculation; Humans2003-12-01
21 Cannon, Grant W.; Clayton, Frederic; Griffiths, Marie M.Passive transfer of adjuvant-induced arthritis into irradiated DA recipient rats.Adjuvant-induced arthritis (AIA) can be passively transferred in Dark Agouti (DA) rats by spleen and lymph node cells after culture with Concanavalin A (Con A). A model not requiring in vitro Con A expansion and activation would be important in investigations of anti-rheumatic drugs in AIA. A new mo...Cell Transplantation; Rats, Inbred Strains1993
22 Jaskowski, Troy D.Comparison of capillary zone and immunosubtraction with agarose gel and immunofixation electrophoresis for detecting and identifying monoclonal gammopathiesCapillary zone electrophoresis (CZE) and immunosubtraction electrophoresis (ISE) were evaluated for ability to detect and immunotype monoclonal proteins, compared with agarose gel electrophoresis (AGE) and immunofixation electrophoresis (IFE), respectively. Six hundred seventeen serum samples wer...Capillary zone electrophoresis; Immunosubtraction electrophoresis; CZE; ISE; Monoclonal protein electrophoresis; Serum protoin electrophoresis; Agarose gel; Dysproteinemias1999
23 Herrmann, Mark G.; Durtschi, Jacob; Bromley, L. Kathryn; Voelkerding, Karl V.Cross platform comparison of melting curve analysisMelting analysis of PCR product was first performed on the LightCycler 10 years ago. Now, melting analysis is a standard function on all real-time PCR instruments. Recent advances in DNA melting analysis, including high resolution melting and specialized dyes, have increased the capabilities of melt...PCR; SNP; SYBR Green I; LC Green Plus; high-resolution melting; mutation scanning; amplicon genotyping2005-11-04
24 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.; Comanor, Lorraine; Di Canzio, JamesEvaluation of the Versant ? HBV DNA 3.0 Assay (bDNA)+ and comparison with the Cobastm HBV Taqman Analyte Specific Reagent Assay.To evaluate the performance characteristics of the Versant ? HBV DNA 3.0 assay (bDNA)+ (Versant, Bayer Healthcare LLC, Tarrytown, NY). 2.) To compare the Versant assay to the COBAS HBV TaqMan Analyte Specific Reagent assay (TaqMan) (Roche Molecular Systems, Pleasanton, CA) using random and defined p...International Standard; HBV DNA Tests2005-05-07
25 Hillyard, David R.Quantitative real time PCR assay for detecting BK virus in serum, plasma and urine.BK is a non-enveloped virus in the Polyomavirus family, closely related to SV40 and JC virus. Primary infection with BK generally occurs during childhood without specific symptoms, and is widespread in the population, with approximately 80% of adults infected globally. The virus remains latent in th...Quantitative Tests; Linearity Studies; Oligonucleotides2005-05-02
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