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1 Tertiary villi (human placenta, late midpregnancy): (A) Left: stain: Hematoxylin - azophloxine. (B) Right: electron microscopy (low magnification). At the left (A) multinucleated syncytiotrophoblast cells (STCs, 1) and vaguely cytotrophoblast cells (CTCs, 2) cover the loose fetal stroma (4). A large and a small arteriole (3) are embedded with stro...placenta; tertiary villi; placental barrier; Hofbauer cell; vasculosyncytial membrane; syncytiotrophoblastPoja Histology Collection - Placenta
2 Survey of implanted blastocyst (human, ca. 3 weeks-old embryo) and detail of advanced stage of decidua cells (human, mid pregnancy)Stain: Hematoxylin-eosin (A, left) and Azan (B, right). (A) Left: section through a slightly damaged implantation site shows at (1) the uterine lumen and (2) the decidua capsularis while at (3) several uterine glands are visible. At arrow (4) the amniotic cavity, (5) points to embryonic shield and...placenta; uterus; syncytiotrophoblast; implantation; blastocystPoja Histology Collection - Placenta
3 Tertiary villi (human placenta, midpregnancy)Stain: Hematoxylin - azophloxine. (A) Left a variety of tertiary villi and reddish-stained fibrinoid depositions (1). At (2): the embryonic connective tissue starts to fibrinize. Note syncytiotrophoblast knots (3) or sprouts of several villi .These knots might represent aggregations of degenerati...placenta; fibrinoid; cytotrophoblast; syncytiotrophoblastPoja Histology Collection - Placenta
4 Syncytiotrophoblast cells of tertiary villi (human placenta, midpregnancy)(A) Left: stain: Immunoperoxidase staining with diaminobenzidin (DAB) and human anti-chorionic gonadotrophin antibody (hCG-DAKO 231), counterstained with hematoxylin. (B) Right: electron microscopy. (A) At the left the exclusive reactivity (dark brown) in the cytoplasma of the multinucleated syn...Syncytiotrophoblast; placenta ; chorionic villi; HCG; immunohistochemistry; electron microscopyPoja Histology Collection - Placenta
5 Electron microscopy of tertiary villus (human placenta, early pregnancy)At the left (A), part of a tertiary (terminal) villus with the multinucleated syncytiotrophoblast cell (1) (STC) at the top. The apex shows protrusions and extensive microvilli of varying sizes (brushborder). Nuclei (1a) are sectioned at different levels and the cytoplasm contains abundant organelle...placenta; chorionic villi; placental barrier; syncytiotrophoblast; electron microscopyPoja Histology Collection - Placenta
6 Electron microscopy of syncytiotrophoblast cells in tertiary villus (human placenta, midpregnancy)The left photograph (A) shows the apical cytoplasm of a syncytiotrophoblast cell (STC, nucleus). The free cell surface displays small protrusions and a characteristic pattern of differently shaped microvilli; pinocytotic invaginations and a single macropinocytotic vacuole. Note the apical localized ...placenta; tertiary villi; syncytiotrophoblast; electron microscopyPoja Histology Collection - Placenta
7 Survey of the chorionic plate and intervillous space (human placenta, full-term)Stain: Perjodic acid-Schiff reaction (PAS). At the top the chorionic plate (1) with cross-sections of umbilical vessels (2) At (3) the folded amnion covering the chorionic plate. Ramifications of thicker stem villi demonstrate free-floating terminal villi (tertiary). At several places reddish-st...placenta; tertiary villi; decidua; chorionic platePoja Histology Collection - Placenta
8 Stem villus (human placenta, midpregnancy)Stain: Trichrome (Goldner). At the top the fetal site with part of the chorionic plate (1) with a thick stem villus (2) ramifying in two smaller villi with large blood vessels (3). The chorionic plate consists of a thick fibrous connective layer (green). Towards the intervillous space it is covere...placenta; chorionic villi; stem villus; chorion platePoja Histology Collection - Placenta
9 Spiral artery in endometrium (human, midpregnancy)Stain: Hematoxylin-eosin. (A) Emerging into partly damaged intervillous space (1), filled with hemorrhage and fibrin (2). (3) uterine vein and (4) smooth muscle cell bundles at the endometrial-myometrial junction. (B) Lumen with hypertrophic endothelium at higher magnification (5). Note as an unu...placenta; trophoblast; spiral artery; endometriumPoja Histology Collection - Placenta
10 Choriocarcinoma (human)Stain: Hematoxylin-eosin. (A) Inset: Survey tumor. Within the uterus (1) a choriocarcinoma forms solitary or multiple nodules composed of hemorrhagic necrotic areas (2) surrounded by neoplastic cells. It resembles an early implanted blastocyst with aggregations of mononuclear lighter-stained cyt...choriocarcinoma; placenta; uterus; cytotrophoblast; GTD (gestational trophoblastic disease)Poja Histology Collection - Placenta
11 Electron microscopy of tertiary villus (human placenta, midpregnancy)In the left photograph (A) is shown part of a tertiary villus with the organelle-rich cytoplasm of a syncytiotrophoblast cell (STC, 1). Below a single electron-light linked cytotrophoblast cell (CTC or Langhans cell, 2) covered by the STC. A higher magnification of another CTC (right photograph) sh...placenta; tertiary villi; syncytiotrophoblast; electron microscopy; placental barrierPoja Histology Collection - Placenta
12 Normal term placenta with umbilical cord attached to the fetus (human)The macroscopy shows the expelled placenta as a discoidal mass with a circular outline about 15-20 cm in the ruptured amnionitic and chorionic sacs. Amnion and smooth chorion (chorion leave) are fused and continuous with the margins of the placenta. The fetal surface (inner surface) is covered by...placenta; amnion; chorion; umbilical cord; fetusPoja Histology Collection - Placenta
13 Survey of the intervillous space with villi (human placenta, full-term)Stain: Perjodic Acid Schiff reaction (PAS). At the right and left side the chorionic plate (1) with few cross-sectioned umbilical vessels (2), at (3) a detached amnion. Cross-sections of thick stem villi (4) at the fetal side ramify into terminal villi (tertiary) that are found stuffed (due to fix...placenta; stem villus; syncytiotrophoblast; amnionPoja Histology Collection - Placenta
14 Chorioamnionitis (human)Histologically chorioamnionitis describes the progression of the inflammatory process. Bacteria firstly colonized the chorioamniotic surface. In first two days polymorphonuclear granulocytes (PMN) migrate to the chorion (chorionitis) marginate and adhere to the bottom of the chorionic plate (stage 1...chorioamnionitis; placenta; trophoblastPoja Histology Collection - Placenta
15 Partial hydatidiform mole and invasive mole (human)(A) Macroscopy: The partial mole (1) occupies a large part of the placenta and is distinct from the normal chorionic plate where the umbilical cord (2) inserts eccentrically with branches of the umbilical vessels. The inset shows a circumscript area with swollen transparent grape-like vesicles (chor...partial hydatidiform mole ; chorioadenomaPoja Histology Collection - Placenta
16 Normal term placenta (human)(A) Left (fetal surface): 30-90 cm long umbilical cord (1), remnant of ruptured transparent amnion (2→) , near the eccentric attachment of the umbilical cord to the chorion plate ramification of the umbilical vessels (3) (arteries are smaller than the veins). (B) Right (maternal surface). The su...placenta; amnion; chorion plate; cotyledonPoja Histology Collection - Placenta
17 Survey and detail of the chorionic plate and intervillous space (human placenta, full-term)Stain: (A) Perjodic acid-Schiff reaction (PAS); (B) Hematoxylin-azophloxine. (A) At the top the chorionic plate (1) with cross-sections of umbilical vessels (2). At (3) the folded amnion covering the chorionic plate. Ramifications of thicker stem villi (6) demonstrate free-floating terminal villi ...placenta; chorionic plate; amnion; terminal villiPoja Histology Collection - Placenta
18 Fetal cotyledons (normal term human placenta)After dissection of one placental lobe several cotyledons (2) are visible in (A). Each fetal cotyledon (2) consists of a main stem villus (1) and all its branches. (B) After trypsinization of a cotyledon the tree of arborisation of the stem villus and its branches becomes visible. (By courtesy...placenta; cotyledon; villus; trypsinizationPoja Histology Collection - Placenta
19 Macroscopy of fetus (human)The fetus (1)is completely wrapped in a shiny transparent amnion (2) and closely associated with the brown-coloured placenta (3). (By courtesy of the Museum of Anatomy and Pathology, University Medical Center, St. Radboud University, Nijmegen, The Netherlands)fetus; placenta; amnionPoja Histology Collection - Placenta
20 Surveys/detail of the decidua basalis and intervillous space of the placenta (human placenta, late midpregnancy)Stain: Hematoxylin ?azophloxine (A, C), Trichrome (Goldner) (B). Left pictures (A, B): The maternal side at the bottom consists of a broad zone of the stripped basal decidua (1) intermingled with reddish matrix-type fibrinoid accumulations and streaks (Rohr) (2), close to the anchoring villi (3) an...placenta; chorionic villi; fibrinoid; trophoblast shellPoja Histology Collection - Placenta
21 Chorioangioma (human)Stain: (A) Hematoxylin-eosin (survey) and (B, C) van Gieson. (A) chorioangioma (1), chorionic plate (2), stem villus (3) and villi (5). (B) shows in (1) a large cellular mass with accumulated capillaries. At (2) part of the chorionic plate. Subchorionic fibrinoid stains yellow (4). (C) reveals t...placenta; chorioangioma; villus; chorionic platePoja Histology Collection - Placenta
22 Immunohistochemistry of tertiary villi (human placenta, midpregnancy )Stain: Immunoperoxidase staining with diaminobenzidin (DAB) and hematoxylin counterstaining for keratin 7 (OVTL 12-30 antibody) at the left (A) and for human anti-chorionic gonadotrophin (hCG-DAKO 231antibody) at the right (B). Tertiary villi show a keratin-positive reaction (brown-stained) in all ...placenta; chorionic villi; HCG; syncytiotrophoblast; keratin 7; immunohistochemistryPoja Histology Collection - Placenta
23 Electron microscopy of cytotrophoblast cell (human placenta, early pregnancy)Below a single electron-light cytotrophoblast cell (CTC or Langhans cell, 1) covered by a syncytiotrophoblast cell (STC, 2) with two nuclei. A continuous basal lamina (3) separates the CTC and STC from the embryonic connective tissue. The CTC appears as a blast-like cell with a large electron-l...placenta; tertiary villi; syncytiotrophoblast; placental barrierPoja Histology Collection - Placenta
24 Tertiary villi (human placenta, early pregnancy)Stain: (A, left) Azan. (B, right) Immunoperoxidase staining with diaminobenzidin (DAB) and hematoxylin counterstaining for human anti-chorionic gonadotrophin (hCG-DAKO 231 antibody). (A) shows blue grey-stained postmitotic multinucleated syncytiotrophoblast cells (STC, 1b), partially cut tangential...placenta; chorionic villi; placental barrier; HCGPoja Histology Collection - Placenta
25 Electron microscopy of syncytiotrophoblast knot in tertiary villus (human placenta, almost full-term)Electron microscopy. The mature villus, surrounded by intervillous space (1), contains capillaries (2) with erythrocytes and pericytes (3) embedded in fetal connective tissue elements (4). Two capillaries are localised close to the covering multinucleated syncytiotrophoblast cell (STC, 5) (vasculosy...placenta; chorionic villi; syncytial knot; electron microscopy; vasculosyncytial membranePoja Histology Collection - Placenta
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