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1 Coffin, Cheryl M.; Capecchi, Mario R.Alveolar rhabdomyosarcomas in conditional Pax3:Fkhr mice: cooperativity of Ink4a/ARF and Trp53 loss of function.Alveolar rhabdomyosarcoma is an aggressive childhood muscle cancer for which outcomes are poor when the disease is advanced. Although well-developed mouse models exist for embryonal and pleomorphic rhabdomyosarcomas, neither a spontaneous nor a transgenic mouse model of alveolar rhabdomyosarcoma has...Cell Differentiation; Forkhead Transcription Factors; Myogenic Regulatory Factors2004-11-01
2 Vaughn, Cecily P.; Crockett, David K.; Lim, Megan S.; Elenitoba-Johnson, Kojo S.J.Analytical characteristics of ICAT-LC-MS/MS for quantitative proteomics studiesRecent studies have employed isotope-coded affinity tags (ICAT) in concert with tandem mass spectrometry (MS/MS) to assess differential expression of proteins on a proteomic scale and perform unbiased quantitative comparisons between samples from different physiological or disease states. We sought ...isotope-coded affinity tags; ICAT; mass spectrometry; MS/MS; proteomic scale; FL2005-06-03
3 Litwin, Christine M.Anti-tuberculosis IgG antibodies as a marker of active mycobacterium tuberculosis diseaseAnti-Mycobacterium tuberculosis IgG antibodies may aid in the diagnosis of active M. tuberculosis disease. We studied whether anti-M. tuberculosis IgG antibodies are elevated in active M. tuberculosis disease and assessed factors contributing to false positive and negative results. A retrospective s...2012-01-01
4 Petti, Cathy A.; Polage, Christopher R.Assessment of the utility of viral culture of cerebrospinal fluidNucleic acid amplification testing is the preferred method to detect enteroviruses and Herpesviridae in cerebrospinal fluid, but clinicians still request viral culture. Review of 22,394 viral cultures of cerebrospinal fluid samples found that <0.1% recovered nonenterovirus, non-Herpesviridae species...Enterovirus Infections; Herpesviridae Infections; Virus Cultivation2006-12-15
5 Digre, Kathleen B.; Gouw, Launce G.; Harris, Catherine P.; Haines, John H.; Ptacek, L.J.Autosomal dominant cerebellar ataxia with retinal degeneration: clinical, neuropathologic, and genetic analysis of a large kindred.The autosomal dominant cerebellar ataxias (ADCA) comprise a heterogeneous group of neurologic disorders characterized by degeneration of the cerebellum, spinal cord, and brainstem. Genetic analysis has revealed two loci, SCA1 on chromosome 6p, and SCA2 on chromosome 12q, responsible for some ADCA. W...Retinal Degeneration; Cerebellar Ataxia; Genetic Analysis1994-08
6 Digre, Kathleen B.; Blumenthal, Deborah T.; Cessna, Melissa H.; Gopez, Evelyn V.; Shen, YueCarcinomatous meningitis as the presenting manifestation of gallbladder carcinoma: case report and review of the literatureThe primary tumors that typically cause carcinomatous meningitis include lung cancer, breast cancer, leukemia, lymphoma and melanoma. A variety of neurological signs and symptoms can be seen depending on the extent and location of the meningeal metastasis. Once the diagnosis of carcinomatous meningi...Carcinomatous Meningitis; Gallbladder Carcinoma; Leptomeningeal Metastasis; Meningeal Carcinomatosis; Cranial Neuropathy2004-10
7 Konnick, Eric Q.; Erali, Maria; Ashwood, Edward R; Hillyard, David RComparison of the DIGENE HBV HYBRID CAPTURE II AND ROCHE COBAS Amplicor HBV Monitor Assays.Evaluate and compare the performance characteristics of the COBAS Amplicor HBV Monitor test (Roche Diagnostics Corp.) and the HBV Hybrid capture II (Digene Corporation) assay for the quantification of HBV BNA Levels.Quantitative Tests; Linearity Studies2001-08-19
8 Konnick, Eric Q.; Erali, Maria; Ashwood, Edward R.; Hillyard, David R.Comparison of the National Genetics Institute (NGI) HCV Superquant and Roche COBAS Amplicor HCV Monitor, Version 2.0 AssaysChronic Hepatitis C infection is currently recognized as an important health care problem. It is estimated that nearly 4 million Americans are infected, 15 to 20 percent of whom will eventually develop Cirrhosis. Current treatments for Hepatitis C virus (HCV) are limited to alfa-interferon alone or ...Cirrhosis; Patients2000-04-22
9 Kriesel, John D.; Baringer, J R; Spruance, Spotswood L.Correlation between detection of herpes simplex virus in oral secretions by PCR and susceptibility to experimental UV radiation-induced herpes labialis.We examined the oral secretions of 25 patients for herpes simplex virus (HSV) at the time of and following experimental UV radiation (UVR). HSV was detected in one or more oral secretion specimens in 5 of 12 (42%) cases by cell culture and in 8 of 12 (67%) cases by PCR. On the day of UVR, HSV was de...Polymerase Chain Reaction; Ultraviolet Rays; Simplexvirus1994
10 Herrmann, Mark G.; Durtschi, Jacob; Bromley, L. Kathryn; Voelkerding, Karl V.Cross platform comparison of melting curve analysisMelting analysis of PCR product was first performed on the LightCycler 10 years ago. Now, melting analysis is a standard function on all real-time PCR instruments. Recent advances in DNA melting analysis, including high resolution melting and specialized dyes, have increased the capabilities of melt...PCR; SNP; SYBR Green I; LC Green Plus; high-resolution melting; mutation scanning; amplicon genotyping2005-11-04
11 Cloud, Joann L.; Hoggan, Karen; Cohen, Samuel; Brown-Elliott, Barbara A.; Mann, Linda; Wilson, Rebecca; Aldous, Wade K.; Wallace, Jr, Richard J.; Woods, Gail L.Differentiation of mycobacterium chelonae and M. abscessus using SmartCycler PCR and MGB eclipse probesThis poster discusses how the use of partial 16S rDNA sequencing (first one-third of the gene) for the identification of Mycobacterium species from cultured isolates has become recognized as a very accurate method for identification. The distinction between M. chelonae and M. abscessus, however, is ...MGB Eclipse System; SmartCycler PCR; Mycobacterium abscessus2005-09-05
12 Pounder, June I.; Voelkerding, Karl V.; Storts, D.; Anderson, Clint M.Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysisDifferentiation of members within Mycobacterium tuberculosis complex (MTC) is essential for patient management and infection control. MTC is comprised of M. tuberculosis (Mtb), M. bovis (Mb), M. bovis BCG (vaccine strain; Mbcg), M. africanum (Ma), and M. microti (Mm). Because MTC have highly similar...MTC; species differentiation; Mycobacterium bovis BCG; Mycobacterium africanum; Mycobacterium microtic; PCR2006-01-09
13 Aldous, Jay A.; Engar, Richard C.Do dentists prescribe narcotics excessively?Dealing with pain is an inevitable sequela to dental treatment. Although several drug regimens primarily involving narcotics have been used in the past, availability of nonsteroidal anti-inflammatory drugs (NSAIDs) has increased recently. A study was conducted to analyze dental prescribing patterns ...Analgesics, Opioid; Anti-Inflammatory Agents; Dentist's Practice Patterns; Pain, Postoperative1996-07-01
14 Parker, Sam; Stevenson, Jeffery B.; Hillyard, David R.Eclipse probe assay for the detection of mycoplasma pneumoniae.Mycoplasma pneumoniae is an important human pathogen that causes community acquired pneumonias and upper respiratory tract infections. Traditional detection techniques, such as culture and serology, cannot rapidly aid in the diagnosis of an acute infection with adequate sensitivity and specificity. ...Eclipse Hybridization Probe; Protocol; Techniques2005-09-11
15 Normann, Richard A.; Chandler, John P.Effects of calcium ions on L-type horizontal cells in the isolated turtle retinaA technique by which the retina can be isolated from the turtle eye is described. Scanning electron microscopy revealed morphological variability between preparations and also between regions of the same one. Large areas were often totally free of any pigment epithelial cells, yet contained a high p...Retina; Calcium; Horizontal Cell; Turtle1990
16 Erickson, J. Alan; Aldeen, William E.; Ashwood, Edward R.Evaluation of a fecal pancreatic elastase-1 enzyme-linked immunosorbent assay: assessment versus an established assay and implication in classifying pancreatic functionDisagreement continues regarding the two commercially available fecal pancreatic elastase-1 (PE-1) ELISAs and their respective capabilities to assess pancreatic function. Our objectives were to validate the newer PE-1 ELISA and evaluate the test against the previously established assay, to investiga...PE-1; pancreatic elastase-1; FDA; AMR; analytical measurement range; BioServ Diagnostics PE-1ELISA; ScheBo Biotech PE-1ELISA2005-09-25
17 Erickson, J. Alan; Ashwood, Edward R.Evaluation of an enzyme-linked binding protein assay for hyaluronic acid and concentrations in hepatitis C infected patientsSerological hyaluronic acid (HA) has been proposed as a noninvasive alternative to liver biopsy for assessing the extent of liver fibrosis. Liver biopsy correctly identifies hepatic disease in about 65 to 75% of cases, being strongly dependent on the length of the biopsy obtained. Furthermore, hepat...Hyaluronic Acid Concentration; Corgenix HA assay2005-07-21
18 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.Evaluation of the Alliance HCV Quantitative Analyte Specific Reagent (ASR) Assay and Comparison to the COBAS HCV TaqMan ASR Assay.To evaluate the Abbott/Celera Alliance HCV Quantitative ASR (Alliance) assay using the Qiagen BioRobot 9604 and QiaAmp Virus Kit for viral RNA isolation. And compare Alliance HCV TaqMan ASR to the Roche COBAS HCV TaqMan ASR (CTM) assay calibrated using Armored RNA solutions and WHO 2nd international...Quantitative Tests; Linearity Studies2004-05-10
19 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) and COBAS HCV TaqMan ASR Assays using the Vidiera NSP sample preparation instrument.The Vidiera Nsp nucleic sample preparation instrument (Vidiera,Beckman-Coulter Inc., Fullerton CA) was evaluated using the COBAS HBV TaqMan Analyte Specific Reagent (ASR. Roche Molecular Diagnostics [RMD], Indianapolis, IN) and COBAS HCV TaqMan ASR (RMD) assays using clinical samples and HCV Armored...Quantitative Tests; Linearity Studies2006-05-05
20 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) Assay and Comparison to the COBAS Amplicor HBV Monitor Assay.erformance characteristics of the COBAS HBV TaqMan ASR (TaqMan) assay were evaluated and compared to the COBAS Amplicor HBV Monitor test (Amplicor) assay for the quantification of HBV DNA in clinical samples.Quantitative Tests; Linearity Studies2004-05-03
21 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.; Comanor, Lorraine; Di Canzio, JamesEvaluation of the Versant ? HBV DNA 3.0 Assay (bDNA)+ and comparison with the Cobastm HBV Taqman Analyte Specific Reagent Assay.To evaluate the performance characteristics of the Versant ? HBV DNA 3.0 assay (bDNA)+ (Versant, Bayer Healthcare LLC, Tarrytown, NY). 2.) To compare the Versant assay to the COBAS HBV TaqMan Analyte Specific Reagent assay (TaqMan) (Roche Molecular Systems, Pleasanton, CA) using random and defined p...International Standard; HBV DNA Tests2005-05-07
22 Wittwer, Carl T.Genotyping accuracy of high-resolution DNA melting instrumentsHigh-resolution DNA melting is a closed-tube method for genotyping and variant scanning that depends on the thermal stability of PCR-generated products. Instruments vary in thermal precision, sample format, melting rates, acquisition, and software. Instrument genotyping accuracy has not been assesse...2014-01-01
23 Liew, Michael; Erali, Maria; Page, Sam; Hillyard, David R.; Wittwer, Carl T.Hepatitis C genotyping by denaturing high-performance liquid chromatographyDetermination of the hepatitis C virus (HCV) genotype for infected patients increasingly has become accepted as the standard of care. Genotype assignment helps in assessing disease prognosis and assists in establishing the appropriate duration of treatment. The great genetic diversity of HCV, with 1...Base Sequence; 5' Untranslated Regions; Genotype; Hepacivirus; Molecular Sequence Data2004-01
24 Dwight, Zachary LawrenceHeterozygote PCR product melting curve predictionMelting curve prediction of PCR products is limited to perfectly complementary strands. Multiple domains are calculated by recursive nearest-neighbor thermodynamics. However, the melting curve of an amplicon containing a heterozygous single nucleotide variant (SNV) after PCR is the composite of four...2014-01-01
25 Erickson, J. Alan; Smith, Jeffrey, J.; Bornhorst, Joshua A.; Ashwood, Edward R.Humananti-mouse antibody protected ELISA for the quantification of squamous cell carcinoma antigenSquamous cell carcinoma antigen (SCC) was first isolated from squamous cell carcinoma tissue of the uterine cervix and initially reported as TA-4. The antigen has been characterized as a glycoprotein with a molecular weight between 45,000 and 55,000 daltons. Studies suggest that SCC may function as ...SCC; Squamous Cell Carcinoma Antigen; BSA; PBS; Phosphate Buffered Saline; HAMA; Human Anti-mouse Antibodies; IIR; ImmunoglobulinInhibiting Reagent2005-12-09
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