Filters: Department:"Human Genetics" Publisher:"Cold Spring Harbor Lab Press" Collection:"ir_uspace"
| Creator | Title | Description | Subject | Date | ||
|---|---|---|---|---|---|---|
| 1 |  | Gesteland, Raymond F.; Ivanov, Ivaylo P.; Atkins, John F. | Programmed frameshifting in the synthesis of mammalian antizyme is +1 in mammals, predominantly +1 in fission yeast but "2 in budding yeast | The coding sequence for mammalian ornithine decarboxylase antizyme is in two different partially overlapping reading frames with no independent ribosome entry to the second ORF. Immediately before the stop codon of the first ORF, a proportion of ribosomes undergo a quadruplet translocation event t... | Antizyme; Frameshifting; Ornithine decarboxylase antizyme; Recoding; S. pombe | 1998 |
| 2 |  | Gesteland, Raymond F.; Atkins, John F. | Origin and destiny of adenovirus proteins | Lytic infection of human cells by adenovirus proceeds by a temporal expression of genes. Classically two phases have been defined: an early phase, which includes events occurring before the onset of DNA synthesis (8 hours), and a late phase, including events whose existence depends on the onset of... | Protein Biosynthesis; DNA, Viral; Adenoviridae; RNA, Viral; Time Factors | 1975 |
| 3 |  | Gesteland, Raymond F.; Atkins, John F. | Evolutionary specialization of recoding: frameshifting in the expression of S. cerevisiae antizyme mRNA is via an atypical antizyme shift site but is still +1 | An autoregulatory translational shift to the +1 frame is required for the expression of ornithine decarboxylase antizyme from fungi to mammals. In most eukaryotes, including all vertebrates and a majority of the studied fungi/yeast, the site on antizyme mRNA where the shift occurs is UCC-UGA. The me... | Antizyme; ODC; Polyamines; Frameshifting | 2006 |
| 4 |  | Hansen, Mark S.; Coffin, Cheryl M.; Capecchi, Mario R. | Pax3:Fkhr interferes with embryonic Pax3 and Pax7 function: implications for alveolar rhabdomyosarcoma cell of origin. | To investigate the role of the translocation-associated gene Pax3:Fkhr in alveolar rhabdomyosarcomas, we generated a Cre-mediated conditional knock-in of Pax3:Fkhr into the mouse Pax3 locus. Exploring embryonic tumor cell origins, we replaced a Pax3 allele with Pax3:Fkhr throughout its expression do... | Neuroprogenitor; Embryogenesis | 2004-11-01 |
| 5 |  | Capecchi, Mario R.; Bernstein, Kenneth E.; Thomas, Kirk R. | Targeting genes for self-excision in the germ line | A procedure is described that directs the self-induced deletion of DNA sequences as they pass through the male germ line of mice. The testes-specific promoter from the angiotensin-converting enzyme gene was used to drive expression of the Cre-recombinase gene. Cre was linked to the selectable marker... | Cre-recombinase; Hoxa3; Chimeric mice | 1999-06-15 |
| 6 |  | Coffin, Cheryl M.; Capecchi, Mario R. | Alveolar rhabdomyosarcomas in conditional Pax3:Fkhr mice: cooperativity of Ink4a/ARF and Trp53 loss of function. | Alveolar rhabdomyosarcoma is an aggressive childhood muscle cancer for which outcomes are poor when the disease is advanced. Although well-developed mouse models exist for embryonal and pleomorphic rhabdomyosarcomas, neither a spontaneous nor a transgenic mouse model of alveolar rhabdomyosarcoma has... | Cell Differentiation; Forkhead Transcription Factors; Myogenic Regulatory Factors | 2004-11-01 |
| 7 |  | Gesteland, Raymond F.; Atkins, John F.; Felden, Brice; McCutcheon, John P. | Probing the structure of the Escherichia coli 10Sa RNA (tmRNA) | The conformation of the Escherichia coli 10Sa RNA (tmRNA) in solution was investigated using chemical and enzymatic probes. Single- and double-stranded domains were identified by hydrolysis of tmRNA in imidazole buffer and by lead(II)-induced cleavages. Ribonucleases T1 and S1 were used to map unpai... | Covariation; Pseudoknot; Structural Probing; 10Sa RNA; tmRNA | 1997 |
| 8 |  | Gesteland, Raymond F.; Atkins, John F.; Baranov, Pavel V. | P-site tRNA is a crucial initiator of ribosomal frameshifting | The expression of some genes requires a high proportion of ribosomes to shift at a specific site into one of the two alternative frames. This utilized frameshifting provides a unique tool for studying reading frame control. Peptidyl-tRNA slippage has been invoked to explain many cases of programmed ... | Translation; Recoding; Kinetic Model; Frameshifting; Ribosome | 2004 |
| 9 |  | Gesteland, Raymond F.; Krapcho, Karen J.; Talbot, Phil; Thulin, Craig | Crystallization of the MS2 translational repressor alone and complexed to bromouridine | The coat protein from the MS2 bacteriophage plays a dual role by encapsidating viral RNA and also by binding RNA as a translational repressor. In order to study the isolated dimer in a conformation not influenced by capsid interactions, a mutant molecule was crystallized that is defective in capsid ... | Crystallization; RNA Bacteriophage; RNA Hairpin; Translational Repressor | 1995 |
| 10 |  | Gesteland, Raymond F. | Characterization of lysozyme messenger and lysozyme synthesized in vitro | In vitro systems for protein systhesis have been in wide use for about 10 years. In most of the early work protein synthesis was measured by following the incorporation of radioactive amino acids into acid precipitable material. This test cannot distinguish between the synthesis of complete, activ... | Lysozyme Messenger; Lysozyme Synthesized; Protein Synthesis | 1969 |
| 11 |  | Gesteland, Raymond F.; Atkins, John F.; Weiss, Robert B. | Slippery runs, shifty stops, backward steps and forward hops: -2, -1, +1, +2, +5 and +6 Ribosomal frameshifting | Frameshift mutations frequently express residual levels of gene activity; that is, they are often leaky. This leakiness can be used as a tool to define the functional components that affect the reading frame during gene expression. Recent technological advances in the capability to efficiently bu... | Molecular Sequence Data; Mutation; RNA, Transfer; Frameshifting | 1987 |
| 12 |  | Gesteland, Raymond F.; Atkins, John F.; Ingram, Jennifer A.; Kelly, Paul J.; Grentzmann, Guido | Dual-luciferase reporter system for studying recoding signals | A new reporter system has been developed for measuring translation coupling efficiency of recoding mechanisms such as frameshifting or readthrough. A recoding test sequence is cloned in between the renilla and firefly luciferase reporter genes and the two luciferase activities are subsequently measu... | Amino Acid Sequence; Genes, Reporter; HIV; Antizyme; Translation | 1998 |
| 13 |  | Gesteland, Raymond F. | Translation of Rl7 RNA fragments | Examination of the events during infection of cells by RNA phages reveals phemonomena that are surprisingly complex for a virus that has only enough information to code for three to four proteins. The coat protein is synthesized at a rapid rate through most of the infectious cycle making it the pre... | Electrophoresis; RNA, Messenger; Peptide Biosynthesis; Kinetics | 1969 |
| 14 |  | Gussin, Gary N.; Capecchi, Mario R. | Protein synthesis directed by DNA phage messengers. | Even through the amino acids corresponding to most of the 64 nucleotide triplets are now known, several important aspects of the genetic code are not yet fully understood. In particular we need more knowledge about the "punctuation marks" of the code-for example, the signals necessary for the initia... | Carbon Isotopes; Escherichia coli; Genetic Code; Methionine | 1967-09-01 |
| 15 |  | Gesteland, Raymond F.; Alam, Steve L.; Nelson, Chad; Felden, Brice; Atkins, John F. | Effects of 3'-terminal phosphates in RNA produced by ribozyme cleavage | During the course of a structural investigation of a selenocysteine insertion element from rat Type 1 iodothyronine 59-deiodinase (D1 SECIS), we wished to prepare milligram quantities of an RNA that comprised the upper stem/loop of this RNA structure (41 nt; Fig+ 1A)+ This stem/loop is a stimul... | In vitro runoff transcription; RNA; Trans-acting ribozymes | 1998 |
| 16 |  | Gesteland, Raymond F.; Atkins, John F.; Howard, Michael T. | Efficient stimulation of site-specific ribosome frameshifting by antisense oligonucleotides | Evidence is presented that morpholino, 2_x0001_-O-methyl, phosphorothioate, and RNA antisense oligonucleotides can direct sitespecific âˆ'1 translational frameshifting when annealed to mRNA downstream from sequences where the P- and A-site tRNAs are both capable of re-pairing with âˆ'1 frame c... | Recoding; Frameshifting; Antisense; Morpholino; Phosphorothioate; 2_x0001_-O-methyl | 2004 |
| 17 |  | Capecchi, Mario R. | Targeted disruption of the even-skipped gene, evx1, causes early postimplantation lethality of the mouse conceptus. | Implantation within the mammalian uterus elicits dramatic changes in the growth, differentiation, and morphogenesis of the conceptus. This process is interrupted in mice carrying a targeted disruption of the murine evx1 gene, a homolog of the Drosophila even-skipped (eve) gene. Upon implantation, pr... | Extraembryonic; Homozygotes; Drosophila | 1994-08-15 |
| 18 |  | Capecchi, Mario R. | Genetic interaction between hoxb-5 and hoxb-6 is revealed by nonallelic noncomplementation. | hoxb-5 and hoxb-6 are adjacent genes in the mouse HoxB locus and are members of the homeotic transcription factor complex that governs establishment of the mammalian body plan. To determine the roles of these genes during development, we generated mice with a targeted disruption in each gene. Three ... | Alleles; CCAAT-Enhancer-Binding Proteins; Cervical Vertebrae; DNA-Binding Proteins; Forelimb; Genetic Complementation Test | 1995-01-01 |
| 19 |  | Capecchi, Mario R. | Housekeeping gene xanthine oxidoreductase is necessary for milk fat droplet enveloping and secretion: gene sharing in the lactating mammary gland. | Xanthine oxidoreductase (XOR) is the rate-limiting enzyme in purine catabolism occurring in most cell types. However, this housekeeping gene is expressed at very high levels in a number of mammalian tissues including the lactating mammary epithelium, suggesting additional roles for XOR in these tiss... | Body Weight; Cell Differentiation; Cell Membrane; Epithelium | 2002-12-15 |
| 20 |  | Capecchi, Mario R.; Tvrdik, Petr | Hoxb1 functions in both motoneurons and in tissues of the periphery to establish and maintain the proper neuronal circuitry. | Formation of neuronal circuits in the head requires the coordinated development of neurons within the central nervous system (CNS) and neural crest-derived peripheral target tissues. Hoxb1, which is expressed throughout rhombomere 4 (r4), has been shown to be required for the specification of facial... | Rhombomere 4; Branchiomotor; Cranial Nerve | 2004-07-04 |
| 21 |  | Capecchi, Mario R. | Hox11 paralogous genes are essential for metanephric kidney induction | The mammalian Hox complex is divided into four linkage groups containing 13 sets of paralogous genes. These paralogous genes have retained functional redundancy during evolution. For this reason, loss of only one or two Hox genes within a paralogous group often results in incompletely penetrant phen... | Metanephric; Six2; Wt1 | 2002-06-01 |
| 22 |  | Capecchi, Mario R. | Characterization of three proteins involved in polypeptide chain termination. | At each stage of elongation, the growing polypeptide chain is bound to the ribosome-messenger RNA complex through the transfer RNA of the most recently incorporated amino acid residue. When the chain is complete, the last polypeptide-transfer RNA (tuna) ester linkage is cleaved, releasing the chain ... | Anti-Bacterial Agents; Phenylalanine; Stimulation, Chemical | 1969 |
| 23 |  | Capecchi, Mario R. | Hoxc13 mutant mice lack external hair | Hox genes are usually expressed temporally and spatially in a colinear manner with respect to their positions in the Hox complex. Consistent with the expected pattern for a paralogous group 13 member, early embryonic Hoxc13 expression is found in the nails and tail. Hoxc13 is also expressed in vibri... | Filiform papillae; Homozygotes; Paralogous | 1998-01-01 |
| 24 |  | Capecchi, Mario R. | Hox genes and mammalian development | We have examined the interactions of Hox genes in forming a cervical vertebrae, hindbrain, and limbs. In each case, it is apparent that individual Hox genes are performing individual functions but that more profound roles are apparent when they act in combination with others Hox genes. The observed ... | Drosophila; Gene Expression Regulation, Developmental; Homozygote | 1997 |
| 25 |  | Gesteland, Raymond F.; Atkins, John F. | Influence of the stacking potential of the base 3' of tandem shift codons on -1 ribosomal frameshifting used for gene expression | Translating ribosomes can shift reading frame at specific sites with high efficiency for gene expression purposes. The most common type of shift to the 1 frame involves a tandem realignment of two anticodons from pairing with mRNA sequence of the form X XXY YYZ to XXX YYY Z where the spaces indicate... | 1 frameshifting; 39 context effect; Codon anticodon interaction; Escherichia coli ; Recoding; tRNALys; XXXY YYZ frameshift motifs | 2002 |