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Creator | Title | Description | Subject | Date |
1 |
 | Gesteland, Raymond F.; Atkins, John F.; Ingram, Jennifer A.; Kelly, Paul J.; Grentzmann, Guido | Dual-luciferase reporter system for studying recoding signals | A new reporter system has been developed for measuring translation coupling efficiency of recoding mechanisms such as frameshifting or readthrough. A recoding test sequence is cloned in between the renilla and firefly luciferase reporter genes and the two luciferase activities are subsequently measu... | Amino Acid Sequence; Genes, Reporter; HIV; Antizyme; Translation | 1998 |
2 |
 | Gesteland, Raymond F.; Alam, Steve L.; Nelson, Chad; Felden, Brice; Atkins, John F. | Effects of 3'-terminal phosphates in RNA produced by ribozyme cleavage | During the course of a structural investigation of a selenocysteine insertion element from rat Type 1 iodothyronine 59-deiodinase (D1 SECIS), we wished to prepare milligram quantities of an RNA that comprised the upper stem/loop of this RNA structure (41 nt; Fig+ 1A)+ This stem/loop is a stimul... | In vitro runoff transcription; RNA; Trans-acting ribozymes | 1998 |
3 |
 | Hughes, Kelly T. | Flagellar anti-σ factor FlgM actively dissociates Salmonella typhimurium σ28 RNA polymerase holoenzyme | The anti-σ factor FlgM of Salmonella typhimurium inhibits transcription of class 3 flagellar genes through a direct interaction with the flagellar-specific σ factor, σ28. FlgM is believed to prevent RNA polymerase (RNAP) holoenzyme formation by sequestering free σ28. We have analyzed FlgM-media... | sigma-factors; transcription; FlgM; Flagellum | 1998 |
4 |
 | Gesteland, Raymond F.; Ivanov, Ivaylo P.; Atkins, John F. | Programmed frameshifting in the synthesis of mammalian antizyme is +1 in mammals, predominantly +1 in fission yeast but "2 in budding yeast | The coding sequence for mammalian ornithine decarboxylase antizyme is in two different partially overlapping reading frames with no independent ribosome entry to the second ORF. Immediately before the stop codon of the first ORF, a proportion of ribosomes undergo a quadruplet translocation event t... | Antizyme; Frameshifting; Ornithine decarboxylase antizyme; Recoding; S. pombe | 1998 |