Filters: Publication Type:"thesis" Department:"Pathology" Collection:"ir_etd" School Or College:"School of Medicine"
| Title | Author | Subject | Description | Date | ||
|---|---|---|---|---|---|---|
| 1 |  | DNMT3A and TET2 program CD4+ T cell differentiation and function during chronic viral infection | Nielson, Monyca Thomas | chronic infection; DNMT3a; epigenetic; TET2 | In response to viral infection, CD4+ helper T cells provide critical signals to provide help for B cell and CD8+ cytolytic T cell responses that can help eliminate the infectious pathogen. However, during chronic viral infections, persistent antigen results in T cell exhaustion, which is characteriz... | 2019 |
| 2 |  | Restoration of deficient P53 function in li-fraumeni syndrome fibroblasts through elephant P53 (EP53) expression | Moore, Kristina Marie Hodson | elephant p53; EP53; Li-fraumeni syndrome; TP53 | The tumor suppressor gene TP53 plays a key role in the maintenance of genomic stability of cells. Following cellular insult, the p53 protein engages several mechanisms to allow the cell to repair the damage or to signal the cell for death, protecting the organism on a cellular level from mutation ac... | 2019 |
| 3 |  | Alternative treatment options for cryptococcal meningitis: the search for amphotericin B synergists | Clifford, Jeffrey Jordan | Cryptococcal meningitis; oseltamivir phosphate; immunosuppressed individuals | Cryptococcal meningitis is a fatal disease that is most commonly found in immunosuppressed individuals, particularly HIV patients. Initial treatment is with a combination of Amphotericin B (AmB) and 5-Flucytosine. 5-flucytosine is expensive and unavailable in many of the countries where the burden o... | 2019 |
| 4 |  | Neonatal sepsis and the characterization and isolation of cord blood myeloid derived suppressor cells | Eason, Rachael Michele | immunology | Millions of neonates die each year worldwide with almost half of these deaths caused by severe infections resulting in sepsis. Preterm infants have higher rates of sepsis and may be at increased risk of severe complications, including death. The reports on frequency of early onset sepsis (EOS) and l... | 2018 |
| 5 |  | The role of ABCB10 in hemoglobinization and arginine metabolism | Miljkovic, Marisa | abcb10; arginine; hemoglobin; murine erythroleukemia cells | ATP-binding cassette (ABC) subfamily B member 10 (Abcb10) is a mitochondrial ABC protein that is important in red cell hemoglobinization. Abcb10 is located on the mitochondrial inner membrane and is suggested to export an unknown substrate out of the mitochondria. Loss of Abcb10 is embryonic lethal,... | 2019 |
| 6 |  | The role of mitoferrins in fatty liver disease | Earl, Aubree Michelle | iron metabolism; lipid metabolism; low iron; mitoferrin 2; NAFLD; non alcoholic fatty liver disease | Non-Alcoholic Fatty Liver Disease (NAFLD) is characterized by the accumulation of fat in the liver, which causes hepatic inflammation, fibrosis, and scarring. This scarring results in decreased liver function, such as hepatic encephalopathy and hepatorenal failure. NAFLD is found in approximately 30... | 2019 |
| 7 |  | Consequences of MU opioid receptor agonism on tyrosine Kinase inhibitor activity in PH+ Chronic Myelogenous Leukemia | Satterlee, Trang Thanh | opioid receptor agonism; tyrosine kinase inhibitors; myelogenous leukemia; mu opioid receptor | Opioid receptor agonism has been shown to promote resistance to, or synergism with, conventional chemotherapeutics that are dependent upon malignant cell type. Pharmacodynamic interactions between newer molecularly targeted chemotherapeutic agents, such as tyrosine kinase inhibitors (TKIs), and opio... | 2019 |
| 8 |  | Disrupted CXCR2 signaling on oligodendroglia lineage cells enhances myelin repair in a viral model of multiple sclerosis | Eckman, Emily | Cxcr2; multiple sclerosis; myelin; oligodendroglia; viral model | Cxcr2 is a chemokine receptor expressed on oligodendroglia that has been implicated in the pathogenesis of neuroinflammatory demyelinating diseases as well as enhancing the migration, proliferation, and myelin production by oligodendroglia. Using an inducible proteolipid protein (Plp) promoter-drive... | 2019 |
| 9 |  | Clinical laboratory utility of Mass Cytometry | Charlton, Cheryl Michelle | Mass cytometry; fluorescence cytometry; common variable immunodeficiency | Mass cytometry is a newly emerging technology that may play a pivotal role in the clinical laboratory. By combining time-of-flight mass spectrometry with fluorescence cytometry methodologies, mass cytometry has already surpassed the current capabilities of fluorescence cytometry. Although mass cytom... | 2019 |
| 10 |  | Experimental and Natural Western Equine Encephalitis virus infections in Reptiles | de St. Jeor, Stephen Charles | virus diseases; encephalitis; reptiles | The production of hemagglutination inhibition (HI) and neutralizing antibodies in garter snakes (Thamnophis elegans) following infection with Western equine encephalitis (WEE) virus was investigated. The duration and magnitude of antibody produced and the effect of temperature on antibody formation ... | 1969 |
| 11 |  | An audit of repeat testing at an academic medical center: consistency of order patterns with recommendations and potential cost savings | Hueth, Kyle Dean | Medicine; health sciences; health care management | We aimed to evaluate the prevalence of potentially unnecessary repeat testing (PURT) and the associated economic burden for an inpatient population at a large academic medical facility. For the target tests, we evaluated all inpatient test orders during 2016 for PURT by comparing the inter-test tim... | 2018 |
| 12 |  | Optimization of crispr/CAS9 technology to study HIV latency in primary CD4+ T cells | Shakya, Binita | Medicine | HIV-1 latently infected cells are the major hurdle impeding viral eradication despite the development of ART (Anti-retroviral therapy), which works by inhibiting various viral proteins necessary for HIV-1 replication. Even after years of daily regimens of ART therapy, HIV-1 reemerges once the ART is... | 2018 |
| 13 |  | Validation of beta-glucosidase activity assay in Leukocytes | Brown, Claudia Cisneiros | biological sciences; Gaucher disease; glucocerebrosidase; lysosomal storage disorders | Gaucher disease (GD) is an autosomal recessive disorder caused by deficiency of the lysosomal hydrolase glucocerebrosidase (GBA) (EC 3.2.1.45), required for the degradation of glycosphingolipids. In Gaucher disease, as a result of GBA deficiency, the body is unable to breakdown glucocerebroside, thu... | 2016 |
| 14 |  | A member of the mycobiome modulates a host metabolite to increase intestinal permeability and disease | Chiaro, Tyson Richard | Microbiology; Immunology | The human body is a fascinating arrangement of cellular organization necessary to support life; it is also home to trillions of microorganisms. The largest concentration of these commensal microbes exists within the gastrointestinal tract (GI) where bacteria dominate the landscape. Accordingly, the ... | 2015-05 |
| 15 |  | The effects of commonly used antibiotic medications on the efficacy of tyrosine kinase inhibitors in Philadelphia Chromosome Positive Leukemia | Zhao, Brooke Marie | Pharmacology; Physiology | The main purpose of this thesis project is to develop and validate a high-throughput protocol to test drug-drug interactions. As a model, tyrosine kinase inhibitors were tested with commonly used antibiotics in Philadelphia chromosome positive leukemia patients. Tyrosine kinase inhibitors (TKI's), s... | 2017 |
| 16 |  | Evaluation of a reductive amination derivatization strategy using aldehydes to improve sensitivity in a plasma catecholamines LC-MS/MS assay | Thaitumu, Marlene Nyambura | Chemistry; Organic chemistry; Medicine | The catecholamines: dopamine, norepinephrine, and epinephrine, are naturally occurring amines that function as hormones and neurotransmitters. Excess concentrations of catecholamines have been observed in patients with rare neuroendocrine tumors and are associated with chronic hypertension, stroke, ... | 2017 |
| 17 |  | Improving peanut allergy diagnosis using specific immunoglobulin E peanut component and basophil activation testing | Wilcock, Diane Marie | health and environmental sciences; basophil activation testing; CD63; flow cytometry; peanut allergy; peanut components; specific immunoglobulin | Peanut allergy is one of the most prevalent and deadliest food allergies. As public awareness has increased about the dangers of food allergies, there has been a corresponding increase in the research on peanut allergies. Significant increases in practical applications of our knowledge have occurred... | 2016 |
| 18 |  | Detection of acetylcholine receptor modulating antibodies by Flow Cytometry | Lozier, Bucky Ken | biological sciences; health and environmental sciences; antigenic modulation; flow cytometry; myasthenia gravis | Myasthenia gravis is an autoimmune disease characterized by antibodies against acetylcholine receptors (AChRs) at the neuromuscular junction (NMJ) of skeletal muscle. These antibodies interfere with the transmission of nerve impulses resulting in weakness and paralysis. Due to the variability in sym... | 2015 |
| 19 |  | Comparison of three methods of detection for group a strep pharyngitis: ILLUMIGENE® group a streptococcus DNA amplification assay, OSOM® ultra strep a test, and routine bacteriologic culture | Pierce, Kristina Bayha | health and environmental sciences; clinical laboratory science; group a strep pharyngitis; infectious disease testing; medical laboratory science; rheumatic fever; strep throat | Rheumatic fever and rheumatic heart disease have been on the decline in the last century, but several episodes of resurgence in the United States and Europe, as well as continued high mortality in developing countries, reinforce the need for continued development of improved methods for detection of... | 2014-12 |
| 20 |  | Simultaneous detection and discrimination of echinococcus granulosus and ecchinococcus multilularis using real-time polymerase chain reaction and high-resolution melting analysis | Olson, Kathryn Mary | biological sciences; health and environmental sciences; echinococcus; high-resolution melting analysis; polymerase chain reaction | Echinococcosis is a zoonotic disease caused by the tapeworm Echinococcus. The two most common species are E. granulosus and E. multilocularis. They cause infections in humans called cystic echinococcosis (CE) and alveolar echinococcosis (AE), respectively. Due to current epidemiological trends, ther... | 2016 |
| 21 |  | A comparison of creatinine measurement by the Jaffe and enzymatic methods in an outpatient population | Adams, Austin Howard | Biochemistry; Medicine | Serum creatinine concentrations and estimated glomerular filtration rates (eGFR) are widely used for the evaluation of renal function. The Jaffe and enzymatic methods are the most common methods for creatinine measurement. The Jaffe method is commonly less expensive than enzymatic methods but may be... | 2014-12 |
| 22 |  | Molecular characteristics of port wine stains | Wright, Karol Lynne | biological sciences; custom capture next generation sequencing panel; digital PCR; GNAQ; port wine stain | Port wine stains are capillary malformations that are typically located in the dermis of the head and neck. They affect 0.3% of the population. Current theories suggest that port wine stains are caused by somatic mutations that disrupt vascular development. I hypothesize that port wine stains are mu... | 2015 |
| 23 |  | Design and validation of a clinical molecular assay for tropheryma whipplei | Chumley, Jeffrey Cramel | Molecular biology; Microbiology | Molecular detection of Tropheryma whipplei is an important tool in the diagnosis of Whipple's Disease and other T. whipplei infections. This thesis describes the design and validation of a real-time polymerase chain reaction assay that detects T. whipplei in cerebrospinal fluid, whole blood, tissue,... | 2017 |
| 24 |  | Development of an assay for the detection of acetylcholine receptor blocking antibodies by Flow Cytometry | Cromar, Anndorie | Pathology; Immunology | Myasthenia Gravis (MG) is a neuromuscular autoimmune disease primarily associated with the presence of antibodies directed toward receptors embedded in the motor end-plate at the neuromuscular junction (NMJ). These antibodies cause blockage, dysfunction, and degradation of acetylcholine receptors (A... | 2014-08 |
| 25 |  | Human lukocyte antigen (HLA) genotyping by next-generation sequencing: development of an in-house method and comparison with commercially available methods | Profaizer, Tracie | Accurate genotyping of the human leukocyte antigens (HLA) are crucial for the success of hematopoietic stem cell and solid organ transplantation. Over the past 50 years, numerous methodologies have been used for HLA typing but technological limitations have prevented full interrogation of the HLA ge... | 2017-08 |