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Filters: Publication Type:"journal article" Collection:"ir_uspace" Type:"Text" Publisher:"American Society for Clinical Pathology" Department:"Pathology"
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TitleCreatorDescriptionSubjectDate
1Diagnostic performance of phospholipid-specific assays for the evaluation of antiphospholipid syndromeDiagnostic performance of phospholipid-specific assays for the evaluation of antiphospholipid syndromeJaskowski, Troy D.The diagnostic performance of commercially available nonstandard antiphospholipid (aPL) assays for the evaluation of antiphospholipid syndrome (APS) is unknown. In 62 patients with APS, 88 with recurrent pregnancy loss, 50 healthy blood donors, and 24 women with one or more successful pregnancies, w...APS; Phospholipid-specific assays; Diagnostic performance2008-06-01
2Screening for antinuclear antibodies by enzyme immunoassayScreening for antinuclear antibodies by enzyme immunoassayJaskowski, Troy D.Indirect fluorescent antibody (IFA) is the most widely used method in clinical laboratories to screen for autoantibodies against a wide variety of nuclear antigens. Recently, a number of antinuclear antibody (ANA) enzyme immunoassay (EIA) screens have become commercially available and claim to be an...Antinuclear antibodies; Enzyme immunoassay1996
3Comparison of four enzyme immunoassays with a western blot assay for the determination of type-specific antibodies to herpes simplex virusComparison of four enzyme immunoassays with a western blot assay for the determination of type-specific antibodies to herpes simplex virusJaskowski, Troy D.Most current enzyme immunoassays (EIAs) differentiate inadequately between types 1 and 2 herpes simplex virus (HSV) antibodies since significant crossreactivity exists. We compared 4 IgG type-specific EIAs using a Western blot assay for resolution of discrepant results. The Diamedix had sensitivit...Enzyme immunoassays; Western blot assay; Type-specific antibodies2001
4Multiplex analysis of heterophil antibodies in patients with indeterminate HIV immunoassay resultsMultiplex analysis of heterophil antibodies in patients with indeterminate HIV immunoassay resultsJaskowski, Troy D.We hypothesized that heterophil antibodies reactive with animal proteins used in blot preparation caused nonspecific staining (NSS) on HIV Western blot (WB) studies, causing indeterminate results. We analyzed samples showing NSS on HIV WB using a multiplexed immunoassay to simultaneously measur...Heterophil antibodies; Heterophil interference; Multiplex analysis; False-positive HIV enzyme-linked immunosorbent assay; ELISA2001
5Effects of hemoglobin C and S traits on the results of 14 commercial glycated hemoglobin assaysEffects of hemoglobin C and S traits on the results of 14 commercial glycated hemoglobin assaysOwen, William E.; Roberts, William L.Glycated hemoglobin is widely used in the management of diabetes mellitus. At least 300,000 Americans with diabetes mellitus have the hemoglobin (Hb) C or S trait. The accuracy of HbA1c methods can be adversely affected by the presence of these traits. We evaluated the effects of HbC and HbS traits ...Glycated hemoglobin assays; Hemoglobin C trait; Hemoglobin S trait2008
6Evaluation of a PCR probe capture assay for the detection of Toxoplasma gondii: incorporation of uracil N-glycosylase for contamination controlEvaluation of a PCR probe capture assay for the detection of Toxoplasma gondii: incorporation of uracil N-glycosylase for contamination controlJaskowski, Troy D.Toxoplasma gondii is a cyst-forming parasite of clinical relevance in humans primarily because of the neurologic abnormalities it can cause. In some clinical circumstances, it is desirable to detect the pathogen directly. We modified a commercially available Toxoplasma polymerase chain reaction...PCR probe capture assay; Uracil N-glycosylase; UNG2000
7Performance characteristics of eight estradiol immunoassaysPerformance characteristics of eight estradiol immunoassaysOwen, William E.; Roberts, William L.Measurement of estradiol is useful in assisted reproduction, evaluation of infertility, menopause, and male feminization. The analytic performance of 8 estradiol immunoassays was evaluated. The imprecision and accuracy of the Access, ADVIA Centaur, ARCHITECT i2000, AutoDELFIA, Elecsys 2010, IMMULI...Estradiol immunoassays2004
8Performance characteristics of four automated natriuretic peptide assaysPerformance characteristics of four automated natriuretic peptide assaysOwen, William E.; Roberts, William L.Measurement of circulating B-type natriuretic peptide (BNP) and N-terminal proBNP (NT-proBNP) can identify patients with heart failure and guide therapy. The limit of detection, linearity, imprecision, method comparison, analytic concordance, and reference intervals of the Access 2 BNP (Biosite, San...Natriuretic peptide assays; BNP2005
9Comparison of capillary zone and immunosubtraction with agarose gel and immunofixation electrophoresis for detecting and identifying monoclonal gammopathiesComparison of capillary zone and immunosubtraction with agarose gel and immunofixation electrophoresis for detecting and identifying monoclonal gammopathiesJaskowski, Troy D.Capillary zone electrophoresis (CZE) and immunosubtraction electrophoresis (ISE) were evaluated for ability to detect and immunotype monoclonal proteins, compared with agarose gel electrophoresis (AGE) and immunofixation electrophoresis (IFE), respectively. Six hundred seventeen serum samples wer...Capillary zone electrophoresis; Immunosubtraction electrophoresis; CZE; ISE; Monoclonal protein electrophoresis; Serum protoin electrophoresis; Agarose gel; Dysproteinemias1999
10Comparison of five automated serum and whole blood folate assaysComparison of five automated serum and whole blood folate assaysOwen, William E.; Roberts, William L.Serum and whole blood folate measurements are used to establish folate deficiency. Most methods used in clinical laboratories are automated, nonisotopic methods that use folate-binding protein. Linearity, imprecision, and method comparison studies, including serum and whole blood hemolysates, wer...Folate assays; Serum folate; Whole blood folate; Automated assays2003
11Performance characteristics of four immunonephelometric assays for the quantitative determination of IgA and IgM in cerebrospinal fluidPerformance characteristics of four immunonephelometric assays for the quantitative determination of IgA and IgM in cerebrospinal fluidOwen, William E.; Roberts, William L.Measurement of IgA and IgM in cerebrospinal fluid (CSF) can be useful in the diagnosis of multiple sclerosis and other central nervous system disorders. The Dade Behring (Deerfield, IL) N Latex IgA and N Latex IgM tests on the BN II System and Beckman Coulter (Brea, CA) low-concentration IgA an...IgA; IgM; Immunonephelometric assays; Imprecision; Method comparison; Reference interval2003
12Determination of cytokine responses using a multiplexed fluorescent microsphere immunoassayDetermination of cytokine responses using a multiplexed fluorescent microsphere immunoassayJaskowski, Troy D.We used a multiplexed fluorescent microsphere immunoassay to develop a sandwich capture assay to assess simultaneously the production of thymus helper (TH) 1- and TH2-type cytokines in tissue culture supernatant obtained from stimulated peripheral blood mononuclear cells. The assay then was use...Multiplexed fluorescent microsphere immunoassay2002
13Polymerase chain reaction detection of Lyme disease: correlation with clinical manifestations and serologic responsesPolymerase chain reaction detection of Lyme disease: correlation with clinical manifestations and serologic responsesJaskowski, Troy D.The Author's have developed a simple, nested polymerase chain reaction (PCR) assay for amplification of an outer surface protein A (OspA) gene fragment of Borrelia burgdorferi using rapid temperature cycling and ethidium bromide detection on agarose gels, and applied it to the diagnosis of Lyme dis...PCR; Rapid cycle amplification1996
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