Peroxidase activity in neutrophilic granulocyte (peripheral blood, guinea pig)

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Title Peroxidase activity in neutrophilic granulocyte (peripheral blood, guinea pig)
Creator Poels, Lambert G.
Contributor Lambert G. Poels, PhD, UMC St Radboud Nijmegen; Paul H. K. Jap, PhD, UMC St Radboud Nijmegen
Date 2007-12-01
Description Electron microscopy (peroxidase reaction with diaminobenzidin staining). The elongated nucleus (3) of this strongly ameboid phagocytic cell has several projections. These nuclear extensions are interconnected by thin heterochromatin strands. The cytoplasm exhibits moderate amounts of organelles, glycogen accumulations (4) and many granules of varying forms, sizes and densities. The primary granules (1) are large spherical to ellipsoid (lightmicroscopic) azurophilic granules and are very electron-dense due to the peroxidase reaction with diaminobenzidin. They contain myeloperoxidase as an antibacterial substance but also acid hydrolases as acid phosphatase, arylsulfatase, beta glucuronidase. The majority of smaller, peroxidase-negative and electron-grey granules are the specific or secondary granules (2). They contain substances like lysozyme, phagocytin to be secreted extracellularly involved in mobilization of inflammatory mediators and complement activation.
Subtype Image
Format image/jpeg
Collection Poja Histology Collection - Blood & Bone Marrow Subset
ARK ark:/87278/s6254mhj
Setname ehsl_heal
Date Created 2012-12-11
Date Modified 2012-12-11
ID 891006
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