Controlled placement of enzymes on carbon nanotubes using comb-branched DNA

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Publication Type pre-print
School or College College of Science
Department Chemistry
Creator Minteer, Shelley D.
Other Author Hausmann, Nina Z.; Baum, Dana A.
Title Controlled placement of enzymes on carbon nanotubes using comb-branched DNA
Date 2014-01-01
Description Immobilization is not only useful for preserving enzyme activity, but also to adhere an enzyme to a surface, such as an electrode, so that the enzyme does not leach into solution during testing. Current immobilization approaches do not readily allow for adjustments to the distance between the enzyme and the electrode or other enzymes. The ability to control the distance of enzymes relative to each other on an electrode can allow for optimal placement and improved current responses. In this report, we investigate the use of comb-branched DNA for enzyme immobilization. A DNA foundation strand was covalently attached to multiwalled carbon nanotubes on a glassy carbon electrode. Comb-branched DNA was then successfully formed using a previously-identified deoxyribozyme to attach DNA strands at specific locations on this foundation strand. By changing the foundation strands, the placement of the DNA strands can be adjusted, allowing for distance changes between the enzyme and the electrode surface. Using standard bioconjugation methods, alcohol dehydrogenase and glucose dehydrogenase were attached to these comb-branched DNA structures, resulting in enzyme immobilization on electrode surfaces. Amperometric analysis revealed both distance and DNA foundation strand length dependence for current response of these enzymes in the presence of their appropriate substrates.
Type Text
Publisher Electrochemical Society
Volume 161
Issue 13
First Page H3001
Last Page H3004
Language eng
Bibliographic Citation Hausmann, N. Z., Minteer, S. D., & Baum, D. A. (2014). Controlled placement of enzymes on carbon nanotubes using comb-branched DNA. Journal of the Electrochemical Society, 161(13), H3001-H3004.
Rights Management © The Electrochemical Society, Inc. 2014. All rights reserved. Except as provided under U.S. copyright law, this work may not be reproduced, resold, distributed, or modified without the express permission of The Electrochemical Society (ECS). The archival version of this work was published in [insert bibliographic information here].
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Reference URL https://collections.lib.utah.edu/ark:/87278/s6n04gn7
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