Tetartohedral twinning in IDI-2 from Thermus thermophilus: crystallization under anaerobic conditions

Update Item Information
Publication Type pre-print
School or College College of Science
Department Chemistry
Creator Poulter, Charles Dale
Other Author De Ruyck, Jerome; Schubert, Heidi L.; Janczak, Matthew W.
Title Tetartohedral twinning in IDI-2 from Thermus thermophilus: crystallization under anaerobic conditions
Date 2014-01-01
Description Type-2 isopentenyl diphosphate isomerase (IDI-2) is a key flavoprotein involved in the biosynthesis of isoprenoids. Since fully reduced flavin mononucleotide (FMNH2) is needed for activity, it was decided to crystallize the enzyme under anaerobic conditions in order to understand how this reduced cofactor binds within the active site and interacts with the substrate isopentenyl diphosphate (IPP). In this study, the protein was expressed and purified under aerobic conditions and then reduced and crystallized under anaerobic conditions. Crystals grown by the sitting-drop vapour-diffusion method and then soaked with IPP diffracted to 2.1 Å resolution and belonged to the hexagonal space group P6322, with unit-cell parameters a = b = 133.3, c = 172.9 Å.
Type Text
Publisher International Union of Crystallography
Volume 70
Issue 3
First Page 347
Last Page 349
Language eng
Bibliographic Citation De Ruyck, J., Schubert, H. L., Janczak, M. W., & Poulter, C. D. (2014). Tetartohedral twinning in IDI-2 from Thermus thermophilus: crystallization under anaerobic conditions. Acta Crystallographica Section F:Structural Biology Communications, 70(3), 347-9.
Rights Management (c) International Union of Crystallography
Format Medium application/pdf
Format Extent 376,410 bytes
Identifier uspace,18902
ARK ark:/87278/s6934394
Setname ir_uspace
ID 712678
Reference URL https://collections.lib.utah.edu/ark:/87278/s6934394
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