Identification of histoplasma -specific peptides in human urine

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Publication Type pre-print
School or College School of Medicine
Department Ophthalmology
Creator Crockett, David K.
Other Author Kushnir, Mark M.; Cloud, Joann L.; Ashwood, Edward R.; Rockwood, Alan L.
Title Identification of histoplasma -specific peptides in human urine
Date 2012-01-01
Description Histoplasmosis is a severe dimorphic fungus infection, which is often difficult to diagnose due to similarity in symptoms to other diseases and lack of specific diagnostic tests. Urine samples from histoplasma-antigen-positive patients and appropriate controls were prepared using various sample preparation strategies including immunoenrichment, ultrafiltration, high-abundant protein depletion, deglycosylation, reverse-phase fractions, and digest using various enzymes. Samples were then analyzed by nanospray tandem mass spectrometry. Accurate mass TOF scans underwent molecular feature extraction and statistical analysis for unique disease makers, and acquired MS/MS data were searched against known human and histoplasma proteins. In human urine, some 52 peptides from 37 Histoplasma proteins were identified with high confidence. This is the first report of identification of a large number of Histoplasma-specific peptides from immunoassay-positive patient samples using tandem mass spectrometry and bioinformatics techniques. These findings may lead to novel diagnostic markers for histoplasmosis in human urine.
Type Text
Publisher Hindawi Publishing Corporation
Volume 2012
First Page 1
Last Page 4
Language eng
Bibliographic Citation Crockett, D. K., Kushnir, M. M., Cloud, J. L., Ashwood, E. R., & Rockwood, A. L. (2012). Identification of histoplasma -specific peptides in human urine. International Journal of Peptides, 2012, 621329, 1-4.
Rights Management (c) Author
Format Medium application/pdf
Format Extent 485,616 bytes
Identifier uspace,18304
ARK ark:/87278/s6v98swk
Setname ir_uspace
Date Created 2013-07-12
Date Modified 2021-05-06
ID 708849
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