Improved Mos1-mediated transgenesis in C. elegans

The ability to add or delete genes to the genome of genetic model organisms is essential. Previously, we developed methods based on the Mos1 transposon1 to make targeted transgene insertions (Mos1-mediated Single Copy transgene Insertions, MosSCI2) and targeted deletions (Mos1-mediated deletions, MosDEL3) in Caenorhabditis elegans, the latter reported in your pages. Here, we present new reagents that improve the efficiency, facilitate the selection for transgenic strains and expand the set of MosSCI insertion sites.