Extraocular ectoderm triggers dorsal retinal fate during optic vesicle evagination in zebrafish

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Publication Type pre-print
School or College School of Medicine
Department Ophthalmology
Creator Fuhrmann, Sabine
Other Author Kruse-Bend, Renee; Rosenthal, Jude; Quist, Tyler S.; Veien, Eric S.; Dorsky, Richard I.; Chien, Chi-Bin
Title Extraocular ectoderm triggers dorsal retinal fate during optic vesicle evagination in zebrafish
Date 2012-01-01
Description Dorsal retinal fate is established early in eye development, via expression of spatially restricted dorsal‐specific transcription factors in the optic vesicle; yet the events leading to initiation of dorsal fate are not clear. We hypothesized that induction of dorsal fate would require an extraocular signal arising from a neighboring tissue to pattern the prospective dorsal retina, however no such signal has been identified. We used the zebrafish embryo to determine the source, timing, and identity of the dorsal retina‐inducing signal. Extensive cell movements occur during zebrafish optic vesicle morphogenesis, however the location of prospective dorsal cells within the early optic vesicle and their spatial relationship to early dorsal markers is currently unknown. Our mRNA expression and fate mapping analyses demonstrate that the dorsolateral optic vesicle is the earliest region to express dorsal specific markers, and cells from this domain contribute to the dorsal retinal pole at 24 hpf. We show that three bmp genes marking dorsal retina at 25 hpf are also expressed extraocularly before retinal patterning begins. We identified gdf6a as a dorsal initiation signal acting from the extraocular non‐neural ectoderm during optic vesicle evagination. We find that bmp2b is involved in dorsal retina initiation, acting upstream of gdf6a. Together, this work has identified the nature and source of extraocular signals required to pattern the dorsal retina.
Type Text
Publisher Elsevier
Dissertation Institution University of Utah
Language eng
Bibliographic Citation Kruse-Bend, R., Rosenthal, J., Quist, T. S., Veien, E. S., Fuhrmann, S., Dorsky, R. I., & Chien, C.-B. (2012). Extraocular ectoderm triggers dorsal retinal fate during optic vesicle evagination in zebrafish. Developmental Biology.
Rights Management (c) Elsevier ; Reprinted from Kruse-Bend, R., Rosenthal, J., Quist, T. S., Veien, E. S., Fuhrmann, S., Dorsky, R. I., & Chien, C.-B. (2012). Extraocular ectoderm triggers dorsal retinal fate during optic vesicle evagination in zebrafish. Developmental Biology. http://dx.doi.org/DOI: 10.1016/j.ydbio.2012.08.004.
Format Medium application/pdf
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Identifier uspace,17746
ARK ark:/87278/s6377th5
Setname ir_uspace
ID 708281
Reference URL https://collections.lib.utah.edu/ark:/87278/s6377th5
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