Eclipse probe assay for the detection of mycoplasma pneumoniae.

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Publication Type Poster
School or College School of Medicine
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Parker, Sam; Stevenson, Jeffery B.; Hillyard, David R.
Title Eclipse probe assay for the detection of mycoplasma pneumoniae.
Date 2005-09-11
Description Mycoplasma pneumoniae is an important human pathogen that causes community acquired pneumonias and upper respiratory tract infections. Traditional detection techniques, such as culture and serology, cannot rapidly aid in the diagnosis of an acute infection with adequate sensitivity and specificity. PCR testing has therefore become a popular testing method. However, lack of a standardized assay that is both rapid and costeffective has discouraged routine clinical testing, leading to misdiagnosis and inappropriate antibiotic therapy. Here we detail a real-time PCR assay for M. pneumoniae detection that utilizes an Eclipse hybridization probe (Nanogen) and targets the P1 cytadeherence gene. We enhanced turnaround time and cost-efficiency by developing a high-throughput protocol that extracts all respiratory sample types simultaneously.
Type Text
Publisher University of Utah
Subject Eclipse Hybridization Probe; Protocol; Techniques
Subject MESH Chemistry, Analytical; Clinical Laboratory Techniques; Immunoassay; Mycoplasma
Language eng
Bibliographic Citation Parker S, Stevenson JB, Hillyard DR. An Eclipse Probe Assay for the Detection of Mycoplasma pneumoniae
Format Medium application/pdf
Identifier ir-main,509
ARK ark:/87278/s69p3kbv
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 707561
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