Detection of targeted GFP-Hox gene fuogenesissions during mouse embry.

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.
Other Author Godwin, Alan R.; Stadler, H. Scott; Nakamura, Kotoka
Title Detection of targeted GFP-Hox gene fuogenesissions during mouse embry.
Date 1998-10-27
Description The ability to use a vital cell marker to study mouse embryogenesis will open new avenues of experimental research. Recently, the use of transgenic mice, containing multiple copies of the jellyfish gene encoding the green fluorescent protein (GFP), has begun to realize this potential. Here, we show that the fluorescent signals produced by single-copy, targeted GFP in-frame fusions with two different murine Hox genes, Hoxa1 and Hoxc13, are readily detectable by using confocal microscopy. Since Hoxa1 is expressed early and Hoxc13 is expressed late in mouse embryogenesis, this study shows that single-copy GFP gene fusions can be used through most of mouse embryogenesis. Previously, targeted lacZ gene fusions have been very useful for analyzing mouse mutants. Use of GFP gene fusions extends the benefits of targeted lacZ gene fusions by providing the additional utility of a vital marker. Our analysis of the Hoxc13(GFPneo) embryos reveals GFP expression in each of the sites expected from analysis of Hoxc13(lacZneo) embryos. Similarly, Hoxa1(GFPneo) expression was detected in all of the sites predicted from RNA in situ analysis. GFP expression in the foregut pocket of Hoxa1(GFPneo) embryos suggests a role for Hoxa1 in foregut-mediated differentiation of the cardiogenic mesoderm.
Type Text
Publisher National Academy of Sciences of the United States of America.
Volume 95
Issue 22
First Page 13042
Last Page 13047
Subject Chimera; Crosses, Genetic; Gestational Age; Green Fluorescent Proteins; Mice, Inbred C57BL; Phenotype
Subject MESH Aging; Blastocyst; Gene Expression Regulation, Developmental; Genes, Homeobox; Homeodomain Proteins; Luminescent Proteins
Language eng
Bibliographic Citation Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13042-7: Godwin AR, Stadler HS, Nakamura K. & Capecchi, MR Detection of targeted GFP-Hox gene fusions during mouse embryogenesis.Retrived on September 15, 2006 from http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9789037
Rights Management National Academy of Sciences of the United States of America. 2000 All Rights Reserved.
Format Medium application/pdf
Identifier ir-main,404
ARK ark:/87278/s69w106b
Setname ir_uspace
ID 707530
Reference URL https://collections.lib.utah.edu/ark:/87278/s69w106b
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