Frameshifting in gene 10 of bacteriophage T7

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Publication Type Journal Article
School or College School of Medicine; College of Science; College of Engineering
Department Human Genetics; Howard Hughes Medical Institute; Bioengineering; Biology
Creator Gesteland, Raymond F.; Atkins, John F.
Other Author Condron, Barry G.
Title Frameshifting in gene 10 of bacteriophage T7
Date 1991
Description Gene 10 of bacteriophage T7, which encodes the most abundant capsid protein, has two products: a major product, 10A (36 kDa), and a minor product, 10B (41 kDa). 10B is produced by frameshifting into the -1 frame near the end of the 10A coding frame and is incorporated into the capsid. The frameshift occurs at a frequency of about 10% and is conserved in bacteriophage T3. This study shows that sequences important to frameshifting include the originally proposed frameshift site, consisting of overlapping phenylalanine codons and the 3' noncoding region that includes the transcriptional terminator over 200 bases downstream of the frameshift site. The frameshift occurs at the overlapping phenylalanine codons as determined from peptide sequencing data. Complementation studies show that there is only a very weak phenotype associated with phage infections in which there is no 10A frameshifting. Capsids from such infections are devoid of 10B and are as stable as wild-type capsids.
Type Text
Publisher American Society for Microbiology
Volume 173
Issue 21
First Page 6998
Last Page 7003
Subject Frameshifting; Bacteriophage T7; RNA, Viral; Gene Expression
Subject MESH Amino Acid Sequence; Capsid Proteins; Molecular Sequence Data; T-Phages
Language eng
Bibliographic Citation Condron, B. G., Atkins, J. F., & Gesteland, R. F. (1991). Frameshifting in gene 10 of bacteriophage T7. Journal of Bacteriology 173(21), 6998-7003
Rights Management ©American Society for Microbiology
Format Medium application/pdf
Identifier ir-main,12771
ARK ark:/87278/s6n87vf0
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-12-06
ID 707512
Reference URL https://collections.lib.utah.edu/ark:/87278/s6n87vf0
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