Molecular characterization of a third member of the guanylyl cyclase-activating protein subfamily

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Publication Type Journal Article
School or College School of Medicine
Department Internal Medicine; Ophthalmology
Creator Baehr, Wolfgang; Rao, Narayanam V.
Other Author Haeseleer, F; Sokal, I; Li, N; Pettenati, M; Bronson, D; Wechter, R; Palczewski, K
Title Molecular characterization of a third member of the guanylyl cyclase-activating protein subfamily
Date 1999
Description The mammalian retina contains at least two guanylyl cyclases (GC1 and GC2) and two guanylyl cyclase-activating proteins (GCAP1 and GCAP2). Here we present evidence of the presence of a new photoreceptor-specific GCAP, termed GCAP3, which is closely related to GCAP1. The sequence similarity of GCAP3 with GCAP1 and GCAP2 is 57 and 49%, respectively. Recombinant GCAP3 and GCAP2 stimulate GC1 and GC2 in low [Ca2+]free and inhibit GCs when [Ca2+]free is elevated, unlike GCAP1, which only stimulates GC1. GCAP3 is encoded by a distinct gene present in other mammalian species but could not be detected by genomic Southern blotting in rodents, amphibians, and lower vertebrates. The intron/exon arrangement of the GCAP3 gene is identical to that of the other GCAP genes. While the GCAP1 and GCAP2 genes are arranged in a tail-to-tail array on chromosome 6p in human, the GCAP3 gene is located on 3q13.1, suggesting an ancestral gene duplication/translocation event. The identification of multiple Ca2+-binding proteins that interact with GC is suggestive of complex regulatory mechanisms for photoreceptor GC.
Type Text
Publisher American Society for Biochemistry and Molecular Biology (ASBMB)
Volume 274
Issue 10
First Page 6526
Last Page 6535
Subject Amino Acid Sequence; Cloning, Molecular; Gene Expression Regulation
Subject MESH Calcium-Binding Proteins; Guanylate Cyclase; Photoreceptors
Language eng
Bibliographic Citation Haeseleer F, Sokal I, Li N, Pettenati M, Rao N, Bronson D, Wechter R, Baehr W, Palczewski K. (1999). Molecular characterization of a third member of the guanylyl cyclase-activating protein subfamily. J Biol Chem, 274(10), 6526-35
Rights Management (c)American Society for Biochemistry and Molecular Biology (ASBMB)
Format Medium application/pdf
Identifier ir-main,1724
ARK ark:/87278/s6j10mvg
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 707420
Reference URL https://collections.lib.utah.edu/ark:/87278/s6j10mvg
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