Pyridine nucleotide metabolism in Escherichia coli. I. Exponential growth

The pyridine nucleotide pool of Escherichia coli is made up almost exclusively of DPN and TPN. In an exponentially growing glucose culture an average cell contains 1,460,000 molecules of DPN (30% reduced) and 440,000 molecules of TPN (57% reduced). If the total volume of the cell were accessible to pyridine nucleotide, this would mean a total DPN concentration of 1.3 X 10-3 M and a TPN concentration of 3.9 X 10-4 M.