Comparison of the cell lines used in meningoma research

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Publication Type Manuscript
School or College School of Medicine
Department Neurosurgery
Creator Couldwell, William T.; Gillespie, David L.; Jensen, Randy L.
Other Author Ragel, Brian T.; Wendland, Merideth M.; Whang, Kum
Title Comparison of the cell lines used in meningoma research
Date 2008
Description Background: Immortal cell lines and cell lines derived from operative specimens transplanted into animal models are used in meningioma research. We address two criticisms of the mouse xenograft flank tumor model: Why are tumor induction rates derived from operative specimens low and inconsistent? Are flank tumors meningiomas? Methods: Meningioma cell cultures were processed for Giemsa-band karyotyping and flow cytometry. Mouse flank tumors induced subcutaneously were analyzed microscopically, immunohistochemically, and ultrastructurally. G-band studies identified meningiomas with simple karyotype (≤ 1 chromosomal abnormality) or complex karyotype (multiple chromosomal abnormalities). Results: Cell cultures with complex karyotypes (IOMM-Lee, CH-157 MN, 2 operative specimens) grew rapidly in vitro and induced tumors in 49/50 (98%) animals. Meningioma cell cultures with simple karyotypes grew slowly in vitro and showed small, non-growing tumors in mouse flanks (10/10). Meningioma flank tumors were vimentin positive with ultrastructural features consistent with meningiomas. Cell cultures with complex karyotypes grew faster in cell culture and consistently induced flank tumors, unlike meningiomas with simple karyotypes. Conclusions: Meningioma cell lines transplanted into flanks of nude mice exhibit microscopic, immunohistochemical, and ultrastructural features of meningiomas. The ease of monitoring tumor growth in the subcutaneous mouse flank model is its primary advantage, although we recognize an intracranial location is more biologically desirable.
Type Text
Publisher Elsevier
Volume 70
Issue 3
First Page 1
Last Page 42
Subject CH-157 MN; IOMM-Lee; Meningioma; Xenograft Mouse Model; Flank tumors
Subject LCSH Meningioma -- Diagnosis; Brain -- Tumors -- Diagnosis; Meningioma -- Genetic aspects -- Animal models; Mice as laboratory animals
Language eng
Bibliographic Citation Ragel, B. T., Couldwell, W. T., Gillespie, D. L., Wendland, M. M., Whang, K., & Jensen, R. L. (2008). Comparison of the cell lines used in meningoma research. Surgical Neurology, 70(3), 295-307.
Rights Management (c) Elsevier
Format Medium application/pdf
Format Extent 1,010,183 bytes
Identifier ir-main,12662
ARK ark:/87278/s6tx4017
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-09-05
ID 707318
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