Isopentenyl diphosphate:dimethylallyl diphosphate isomerase. An improved purification of the enzyme and isolation of the gene from Saccharomyces cerevisiae.

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Publication Type Journal Article
School or College College of Pharmacy; College of Science; School of Medicine
Department Biochemistry; Medicinal Chemistry; Chemistry
Creator Poulter, Charles Dale
Other Author Anderson, Matt S.; Muehlbacher, Manfred; Street, Ian P.; Proffitt, John
Title Isopentenyl diphosphate:dimethylallyl diphosphate isomerase. An improved purification of the enzyme and isolation of the gene from Saccharomyces cerevisiae.
Date 1989-11-15
Description Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase) is an enzyme in the isoprenoid biosynthetic pathway which catalyzes the interconversion of the primary five-carbon homoallylic and allylic diphosphate building blocks. We report a substantially improved procedure for purification of this enzyme from Saccharomyces cerevisiae. An amino-terminal sequence (35 amino acids) was obtained from a highly purified preparation of IPP isomerase. Oligonucleotide probes based on the protein sequence were used to isolate the structural gene encoding IPP isomerase from a yeast lambda library. The cloned gene encodes a 33,350-dalton polypeptide of 288 amino acids. A 3.5-kilobase EcoRI fragment containing the gene was subcloned into the yeast shuttle vector YRp17. Upon transformation with plasmids containing the insert, a 5-6-fold increase in IPP isomerase activity was seen in transformed cells relative to YRp17 controls, confirming the identity of the cloned gene. This is the first reported isolation of the gene for IPP isomerase.
Type Text
Publisher American Society for Biochemistry and Molecular Biology (ASBMB)
Volume 264
Issue 32
First Page 19169
Last Page 19175
Subject Amino Acid Sequence; Base Sequence; Chromatography, Gel; Chromatography, Ion Exchange
Subject MESH Carbon-Carbon Double Bond Isomerases; DNA, Fungal; Genes, Fungal; Saccharomyces cerevisiae
Language eng
Bibliographic Citation J Biol Chem. 1989 Nov 15;264(32):19169-75. Anderson MS, Muehlbacher M, Street IP, Proffitt J, Poulter CD. Isopentenyl diphosphate:dimethylallyl diphosphate isomerase. An improved purification of the enzyme and isolation of the gene from Saccharomyces cerevisiae. Retieved April 16,2007 from http://www.jbc.org/cgi/reprint/264/32/19169.
Rights Management Copyright © 1989 American Society for Biochemistry and Molecular Biology. All rights reserved.
Format Medium application/pdf
Identifier ir-main,937
ARK ark:/87278/s6183r6h
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 707185
Reference URL https://collections.lib.utah.edu/ark:/87278/s6183r6h
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