Targeting genes for self-excision in the germ line

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Internal Medicine; Oncological Sciences; Human Genetics; Biology
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.; Bernstein, Kenneth E.; Thomas, Kirk R.
Other Author Bunting, Michaeline; Greer, Joy M.
Title Targeting genes for self-excision in the germ line
Date 1999-06-15
Description A procedure is described that directs the self-induced deletion of DNA sequences as they pass through the male germ line of mice. The testes-specific promoter from the angiotensin-converting enzyme gene was used to drive expression of the Cre-recombinase gene. Cre was linked to the selectable marker Neor, and the two genes flanked with loxP elements. This cassette was targeted to the Hoxa3 gene in mouse ES cells that were in turn used to generate chimeric mice. In these chimeras, somatic cells derived from the ES cells retained the cassette, but self-excision occurred in all ES-cell-derived sperm.
Type Text
Publisher Cold Spring Harbor Lab Press
Volume 13
Issue 12
First Page 1524
Last Page 1528
Subject Cre-recombinase; Hoxa3; Chimeric mice
Subject MESH DNA Damage; Gene Targeting; Integrases
Language eng
Bibliographic Citation Bunting M, Bernstein K E ,Greer J M, Capecchi genes for self-excision in the germ line. Genes Dev. 1999 Jun 15;13(12):1524-8. Retrieved on September 12, 2006
Identifier ir-main,369
ARK ark:/87278/s6xs6d2z
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 707116
Reference URL
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