Performance characteristics of an HPLC method for Urinary Albumin

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Publication Type Poster
School or College School of Medicine
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Owen, William E.; Roberts, William L.
Title Performance characteristics of an HPLC method for Urinary Albumin
Date 2005-07-06
Description Microalbuminuria is a marker of early diabetic nephropathy and cardiovascular risk. Immunoassays for urinary albumin can underestimate the amount of intact albumin present in urine. The purpose of this study was to evaluate a new urinary albumin assay that employs size exclusion HPLC with UV detection to quantify urinary albumin. We determined the limit of detection (LOD), linearity, imprecision, comparison to an immunoturbidimetric assay (ITA), and pediatric and adult reference intervals on random urine collections. The HPLC assay for total urinary albumin shows acceptable performance and quantifies albumin species not detected by immunoassay. Our studies of apparently healthy children and adults suggest the need for separate male and female and pediatric and adult reference intervals. Further outcome studies are necessary to determine how to best interpret urinary albumin measurements made by HPLC.
Type Text
Publisher Associated and Regional University Pathologists (ARUP) Institute for Clinical and Experimental Pathology
Subject MA; Microalbuminuria; HPLC
Subject MESH Chromatography, High Pressure Liquid; Albuminuria; Urinalysis
Language eng
Rights Management http://creativecommons.org/licenses/by-nc-nd/2.5/
Format Medium application/pdf
Format Extent 161,373 bytes
Identifier ehsl-arup,17
ARK ark:/87278/s634940w
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 706993
Reference URL https://collections.lib.utah.edu/ark:/87278/s634940w
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