Isoprenyl diphosphate synthases: protein sequence comparisons, a phylogenetic tree, and predictions of secondary structure

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Publication Type Journal Article
School or College College of Science
Department Chemistry
Creator Poulter, Charles Dale
Other Author Chen, A.; Kroon, P.A.
Title Isoprenyl diphosphate synthases: protein sequence comparisons, a phylogenetic tree, and predictions of secondary structure
Date 1994
Description Isoprenyl diphosphate synthases are ubiquitous enzymes that catalyze the basic chain-elongation reaction in the isoprene biosynthetic pathway. Pairwise sequence comparisons were made for 6 farnesyl diphosphate synthases, 6 geranylgeranyl diphosphate synthases, and a hexaprenyl diphosphate synthase. Five regions with highly conserved residues, two of which contain aspartate-rich DDXX(XX)D motifs found in many prenyltransferases, were identified. A consensus secondary structure for the group, consisting mostly of a-helices, was predicted for the multiply aligned sequences from amino acid compositions, computer assignments of local structure, and hydropathy indices. Progressive sequence alignments suggest that the 13 isoprenyl diphosphate synthases evolved from a common ancestor into 3 distinct clusters. The most distant separation is between yeast hexaprenyl diphosphate synthetase and the other enzymes. Except for the chromoplastic geranylgeranyl diphosphate synthase from Cupsicum unnuum, the remaining farnesyl and geranylgeranyl diphosphate synthases segregate into prokaryotic/ archaebacterial and eukaryotic families.
Type Text
Publisher Cambridge University Press
Volume 1994
Issue 3
First Page 600
Last Page 607
Subject Catalytic site; Evolution; Farnesyl diphosphate; Geranylgeranyl diphosphate; Prenyltransferase; Substrate binding:
Subject LCSH Biosynthesis; Genetics
Language eng
Bibliographic Citation Chen, A., Kroon, P.A. & Poulter, C.D.(1994). Isoprenyl diphosphate synthases: protein sequence comparisons, a phylogenetic tree, and predictions of secondary structure. Protein Science, 1994(3), 600-7
Rights Management (c)Cambridge University Press
Format Medium application/pdf
Format Extent 1,004,828 Bytes
Identifier ir-main,989
ARK ark:/87278/s6h4292x
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 706837
Reference URL https://collections.lib.utah.edu/ark:/87278/s6h4292x
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