Introduction of a lacZ reporter gene into the mouse int-2 locus by homologous recombination.

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Mansour, Suzanne L.; Thomas, Kirk R.; Capecchi, Mario R.
Other Author Deng, Chuxia
Title Introduction of a lacZ reporter gene into the mouse int-2 locus by homologous recombination.
Date 1990-10
Description We demonstrate that the frequency of gene targeting is unaffected by the length of nonhomologous DNA transferred to a target chromosomal sequence. A result of this finding is that a much wider spectrum of designed genomic alterations is now feasible. As a first application, we inserted a 5.4-kilobase cassette of nonhomologous DNA into the int-2 locus in mouse embryo-derived stem cells by gene targeting. The inserted DNA contained a lacZ gene positioned to create an in-frame fusion with the int-2 protein-coding region. Upon differentiation of these cells to embryoid bodies, the int-2-lacZ fusion faithfully reproduced the expression pattern of int-2 RNA. This ability to target reporter genes, such as lacZ, to specific mouse loci, combined with the ability to move the tagged gene into different mutant backgrounds, may provide an ideal approach for analyzing interactions among genes that participate in a developmental network.
Type Text
Publisher National Academy of Sciences of the United States of America.
Volume 87
Issue 19
First Page 7688
Last Page 7692
Subject Blotting, Southern; Cell Differentiation; Fluorescent Antibody Technique; Restriction Mapping
Subject MESH Hypoxanthine Phosphoribosyltransferase; Recombination, Genetic; Transfection
Language eng
Bibliographic Citation Proc Natl Acad Sci U S A. 1990 Oct;87(19):7688-92: Mansour SL, Thomas KR, Capecchi MR. Introduction of a lacZ reporter gene into the mouse int-2 locus by homologous recombination. Retrieved on September 18.2006 from http://www.pnas.org/cgi/reprint/87/19/7688.
Rights Management National Academy of Sciences of the United States of America. 1999. All Rights Reserved.
Format Medium application/pdf
Identifier ir-main,431
ARK ark:/87278/s6pr8djg
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 706821
Reference URL https://collections.lib.utah.edu/ark:/87278/s6pr8djg
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