Tissue-specific expression in transgenic mice directed by the 5'-flanking sequences of the human gene encoding interphotoreceptor retinoid-binding protein

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Ophthalmology; Neurobiology & Anatomy; Biology
Creator Baehr, Wolfgang
Other Author Liou, Gregory I.; Geng, Li.; Al-Ubaidi, Muayyad R.; Matragoon, Suraporn.; Hanten, Gerri.; Overbeek, Paul A.
Title Tissue-specific expression in transgenic mice directed by the 5'-flanking sequences of the human gene encoding interphotoreceptor retinoid-binding protein
Date 1990-05-25
Description Interphotoreceptor retinoid-binding protein (IRBP) is an extracellular protein that has been suggested to participate in the visual process as a carrier for visual retinoids. A chimeric gene composed of the human IRBP promoter fused to the bacterial reporter gene chloramphenicol acetyltransferase (CAT) was used to generate transgenic mice. Analysis of six transgenic families revealed that the CAT gene, concomitant with the endogenous IRBP gene, was expressed primarily in the retina and, to a lesser extent, in the pineal gland. These results establish that a 1.3-kilobase fragment from the 5' end of the human IRBP gene is sufficient to direct transgene expression to a visual subdivision of the central nervous system.
Type Text
Publisher American Society for Biochemistry and Molecular Biology (ASBMB)
Subject Restriction Mapping; Nucleic Acid Hybridization; Mice; Gene Expression
Subject MESH Tretinoin; Photoreceptors; Organ Specificity; Molecular Sequence Data; Base Sequence
Language eng
Bibliographic Citation Liou GI, Geng L, al-Ubaidi MR, Matragoon S, Hanten G, Baehr W, Overbeek PA. (1990). Tissue-specific expression in transgenic mice directed by the 5
Rights Management (c)American Society for Biochemistry and Molecular Biology (ASBMB)
Format Medium application/pdf
Identifier ir-main,1707
ARK ark:/87278/s6nw02sh
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 706240
Reference URL https://collections.lib.utah.edu/ark:/87278/s6nw02sh
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