Evolutionary specialization of recoding: frameshifting in the expression of S. cerevisiae antizyme mRNA is via an atypical antizyme shift site but is still +1

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Publication Type Journal Article
School or College School of Medicine; College of Engineering
Department Human Genetics; Howard Hughes Medical Institute; Bioengineering; Biology
Creator Gesteland, Raymond F.; Atkins, John F.
Other Author Ivanov, Ivaylo P.
Title Evolutionary specialization of recoding: frameshifting in the expression of S. cerevisiae antizyme mRNA is via an atypical antizyme shift site but is still +1
Date 2006
Description An autoregulatory translational shift to the +1 frame is required for the expression of ornithine decarboxylase antizyme from fungi to mammals. In most eukaryotes, including all vertebrates and a majority of the studied fungi/yeast, the site on antizyme mRNA where the shift occurs is UCC-UGA. The mechanism of the frameshift on this sequence likely involves nearly universal aspects of the eukaryotic translational machinery. Nevertheless, a mammalian antizyme frameshift cassette yields predominantly -2 frameshift in Saccharomyces cerevisiae, instead of the +1 in mammals. The recently identified endogenous S. cerevisiae antizyme mRNA has an atypical shift site: UGC-GCG-UGA. It is shown here that endogenous S. cerevisiae antizyme frameshifting is +1 rather than -2. We discuss how antizyme frameshifting in budding yeasts exploits peculiarities of their tRNA balance, and relate this to prior studies on Ty frameshifting.
Type Text
Publisher Cold Spring Harbor Lab Press
Volume 12
Issue 3
First Page 332
Last Page 337
Subject Antizyme; ODC; Polyamines; Frameshifting
Subject MESH Fungi; Mammals; Molecular Sequence Data; RNA, Messenger
Language eng
Bibliographic Citation Ivanov IP, Gesteland RF, Atkins JF. 2006. Evolutionary specialization of recoding: frameshifting in the expression of S. cerevisiae antizyme mRNA is via an atypical antizyme shift site but is still +1. RNA, 12(3), 332-7
Rights Management © Cold Spring Harbor Laboratory Press-CSHL Press
Format Medium application/pdf
Identifier ir-main,12764
ARK ark:/87278/s68s57fj
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-12-03
ID 706212
Reference URL https://collections.lib.utah.edu/ark:/87278/s68s57fj
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