Purification and characterization of mouse hypoxanthine-guanine phosphoribosyltransferase.

Update item information
Publication Type Journal Article
School or College College of Science; School of Medicine
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.
Other Author Hughes, Stephen H.; Wahl, Geoffrey M.
Title Purification and characterization of mouse hypoxanthine-guanine phosphoribosyltransferase.
Date 1975-01-31
Description Hypoxanthine-guanine phosphoribosyltransferase (HGPR transferase) (EC 2.4.2.8) has been purified approximately 4500-fold to apparent homogeneity from mouse liver. The procedure involves the use of affinity chromatography and was designed to be readily adaptable to small scale isolations. The enzyme appears to be composed of 3 subunits of identical molecular weight (27,000 per subunit). The subunit molecular weight has also been determined by the analysis of radioactively labeled HGPR transferase immunoprecipitated from wild type and mutant (HGPR transferase) mouse tissue culture cell lines.
Type Text
Publisher American Society for Biochemistry and Molecular Biology (ASBMB)
Volume 250
Issue 1
First Page 120
Last Page 126
Subject Buffers; Centrifugation, Density Gradient; Chromatography, Affinity; Chromatography, Gel; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel
Subject MESH Guanine Nucleotides; Liver; Pentosyltransferases; Sulfur Radioisotopes; Ultrafiltration
Language eng
Bibliographic Citation J Biol Chem. 1975 Jan 10;250(1):120-6: Hughes SH, Wahl GM, Capecchi MR. Purification and characterization of mouse hypoxanthine-guanine phosphoribosyltransferase. Retrieved on September 22.2006 from http://www.jbc.org/cgi/reprint/250/1/120.
Rights Management Copyright © 1975 American Society for Biochemistry and Molecular Biology. All Rights Reserved..
Format Medium application/pdf
Identifier ir-main,462
ARK ark:/87278/s6ng585p
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 706124
Reference URL https://collections.lib.utah.edu/ark:/87278/s6ng585p
Back to Search Results